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Structure paper

TitleX-ray crystallographic analysis of time-dependent binding of guanidine hydrochloride to HEWL: First steps during protein unfolding.
Journal, issue, pagesInt. J. Biol. Macromol., Vol. 122, Page 903-913, Year 2019
Publish dateJun 20, 2018 (structure data deposition date)
AuthorsRaskar, T. / Koh, C.Y. / Niebling, S. / Kini, R.M. / Hosur, M.V.
External linksInt. J. Biol. Macromol. / PubMed:30412756
MethodsX-ray diffraction
Resolution1.75 - 1.76 Å
Structure data

PDB-6a4n:
HEWL crystals soaked in 2.5M GuHCl for 8 minutes
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6a4o:
HEWL crystals soaked in 2.5M GuHCl for 20 minutes
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6a4p:
HEWL crystals soaked in 2.5M GuHCl for 40 minutes
Method: X-RAY DIFFRACTION / Resolution: 1.76 Å

PDB-6a4q:
HEWL crystals soaked in 2.5M GuHCl for 110 minutes
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-GAI:
GUANIDINE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • gallus gallus (chicken)
KeywordsHYDROLASE / Protein Unfolding / Chemical denaturant / guanidine / Lysozyme

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