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Open data
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Basic information
Entry | Database: PDB / ID: 5zfo | ||||||
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Title | NMR structure of IRD12 from Capsicum annum. | ||||||
![]() | Pin-II type proteinase inhibitor 38 | ||||||
![]() | PLANT PROTEIN / PROTEIN / inhibitor / Capsicum annum | ||||||
Function / homology | Proteinase inhibitor I20 / Potato type II proteinase inhibitor family / Wheat Germ Agglutinin (Isolectin 2); domain 1 - #30 / Wheat Germ Agglutinin (Isolectin 2); domain 1 / serine-type endopeptidase inhibitor activity / 2-Layer Sandwich / Alpha Beta / Pin-II type proteinase inhibitor 38![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
![]() | Gartia, J. / Barnwal, R.P. / Chary, K.V.R. | ||||||
![]() | ![]() Title: NMR structure and dynamics of inhibitory repeat domain variant 12, a plant protease inhibitor from Capsicum annuum, and its structural relationship to other plant protease inhibitors. Authors: Gartia, J. / Anangi, R. / Joshi, R.S. / Giri, A.P. / King, G.F. / Barnwal, R.P. / Chary, K.V.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 152.7 KB | Display | ![]() |
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PDB format | ![]() | 131.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 395.4 KB | Display | ![]() |
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Full document | ![]() | 446 KB | Display | |
Data in XML | ![]() | 10.4 KB | Display | |
Data in CIF | ![]() | 15.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 5429.053 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution / Contents: 0.6 mM [U-13C; U-15N] IRD12, 90% H2O/10% D2O / Label: 13C,15N / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.6 mM / Component: IRD12 / Isotopic labeling: [U-13C; U-15N] |
Sample conditions | Ionic strength: 25 mM / Label: conditions_1 / pH: 4.0 / Pressure: AMBIENT atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AvanceIII / Manufacturer: Bruker / Model: AvanceIII / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 5 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 200 / Conformers submitted total number: 11 |