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Yorodumi- PDB-5z1n: Crystal structure of C terminal region of G-protein interacting p... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5z1n | ||||||||||||
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Title | Crystal structure of C terminal region of G-protein interacting protein 1 (Gip1) from Dictyostelium discoideum | ||||||||||||
Components | G-protein interacting protein 1 | ||||||||||||
Keywords | PROTEIN BINDING / alpha helix / lipid binding / G-protein binding / social amoebae / chemotaxis | ||||||||||||
Function / homology | Function and homology information chemotaxis to cAMP / establishment of protein localization to plasma membrane / heterotrimeric G-protein binding / G-protein beta/gamma-subunit complex binding / GTPase binding / regulation of apoptotic process / cytosol Similarity search - Function | ||||||||||||
Biological species | Dictyostelium discoideum (eukaryote) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.949 Å | ||||||||||||
Authors | Miyagawa, T. / Koteishi, H. / Kamimura, Y. / Miyanaga, Y. / Takeshita, K. / Nakagawa, A. / Ueda, M. | ||||||||||||
Funding support | Japan, 3items
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Citation | Journal: Nat Commun / Year: 2018 Title: Structural basis of Gip1 for cytosolic sequestration of G protein in wide-range chemotaxis Authors: Miyagawa, T. / Koteishi, H. / Kamimura, Y. / Miyanaga, Y. / Takeshita, K. / Nakagawa, A. / Ueda, M. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5z1n.cif.gz | 56.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5z1n.ent.gz | 36.4 KB | Display | PDB format |
PDBx/mmJSON format | 5z1n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5z1n_validation.pdf.gz | 619.3 KB | Display | wwPDB validaton report |
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Full document | 5z1n_full_validation.pdf.gz | 620.7 KB | Display | |
Data in XML | 5z1n_validation.xml.gz | 10 KB | Display | |
Data in CIF | 5z1n_validation.cif.gz | 13.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/z1/5z1n ftp://data.pdbj.org/pub/pdb/validation_reports/z1/5z1n | HTTPS FTP |
-Related structure data
Related structure data | 5z39C 3f4mS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19318.480 Da / Num. of mol.: 1 / Fragment: ligand binding domain, UNP residues 146-310 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Dictyostelium discoideum (eukaryote) / Gene: DDB0216772 / Plasmid: pE-SUMOstar / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta(DE3) / References: UniProt: Q55BQ2 |
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#2: Chemical | ChemComp-PEF / |
#3: Chemical | ChemComp-LHG / |
#4: Chemical | ChemComp-NA / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.84 Å3/Da / Density % sol: 33.13 % / Mosaicity: 0.871 ° |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 14-20% PEG 20000, 100mM Bicine, / PH range: 8.0-9.0 |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL26B1 / Wavelength: 1 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: RAYONIX MX225HE / Detector: CCD / Date: Sep 20, 2016 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.949→50 Å / Num. obs: 10906 / % possible obs: 98.3 % / Redundancy: 3.8 % / Biso Wilson estimate: 18.34 Å2 / Rmerge(I) obs: 0.09 / Rpim(I) all: 0.054 / Rrim(I) all: 0.105 / Χ2: 1.392 / Net I/σ(I): 8.2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3F4M Resolution: 1.949→48.319 Å / SU ML: 0.21 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 23.35
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 72.88 Å2 / Biso mean: 18.9108 Å2 / Biso min: 3.81 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.949→48.319 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 4
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