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Open data
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Basic information
Entry | Database: PDB / ID: 5y63 | ||||||
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Title | Crystal structure of Enterococcus faecalis AhpC | ||||||
![]() | Alkyl hydroperoxide reductase, C subunit | ||||||
![]() | OXIDOREDUCTASE / 2Cys peroxiredoxins | ||||||
Function / homology | ![]() NADH-dependent peroxiredoxin / NADH-dependent peroxiredoxin activity / peroxiredoxin activity / peroxidase activity / response to oxidative stress / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pan, A. / Balakrishna, A.M. / Grueber, G. | ||||||
![]() | ![]() Title: Atomic structure and enzymatic insights into the vancomycin-resistant Enterococcus faecalis (V583) alkylhydroperoxide reductase subunit C Authors: Pan, A. / Balakrishna, A.M. / Nartey, W. / Kohlmeier, A. / Dip, P.V. / Bhushan, S. / Gruber, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 340.1 KB | Display | ![]() |
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PDB format | ![]() | 280.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4o5rS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 20974.455 Da / Num. of mol.: 5 / Fragment: UNP residues 1-172 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 700802 / V583 / Gene: ahpC, EF_2739 / Plasmid: pET-9d / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.75 Å3/Da / Density % sol: 55.22 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8.5 / Details: Tris HCl pH 8.5, Magnesium Chloride, PEG 400 (30%) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 16, 2015 / Details: Mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.85→30 Å / Num. obs: 25934 / % possible obs: 99.1 % / Redundancy: 3.2 % / Biso Wilson estimate: 56.23 Å2 / CC1/2: 0.992 / Rmerge(I) obs: 0.09 / Net I/σ(I): 8.6 |
Reflection shell | Resolution: 2.85→2.95 Å / Redundancy: 2.2 % / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 1.6 / CC1/2: 0.765 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4O5R Resolution: 2.87→30 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.902 / SU B: 38.752 / SU ML: 0.328 / Cross valid method: THROUGHOUT / ESU R Free: 0.389 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 60.494 Å2
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Refinement step | Cycle: 1 / Resolution: 2.87→30 Å
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Refine LS restraints |
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