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Yorodumi- PDB-5wzo: Crystal structure of human secreted phospholipase A2 group IIE, c... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5wzo | ||||||
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Title | Crystal structure of human secreted phospholipase A2 group IIE, crystallized with calcium | ||||||
Components | Group IIE secretory phospholipase A2 | ||||||
Keywords | HYDROLASE/INHIBITOR / inhibitor / HYDROLASE-INHIBITOR complex | ||||||
Function / homology | Function and homology information Acyl chain remodelling of PG / Acyl chain remodelling of PC / Acyl chain remodelling of PI / Acyl chain remodelling of PS / Acyl chain remodelling of PE / Synthesis of PA / phosphatidylglycerol metabolic process / calcium-dependent phospholipase A2 activity / phosphatidylcholine metabolic process / low-density lipoprotein particle remodeling ...Acyl chain remodelling of PG / Acyl chain remodelling of PC / Acyl chain remodelling of PI / Acyl chain remodelling of PS / Acyl chain remodelling of PE / Synthesis of PA / phosphatidylglycerol metabolic process / calcium-dependent phospholipase A2 activity / phosphatidylcholine metabolic process / low-density lipoprotein particle remodeling / phospholipase A2 / phospholipase A2 activity / arachidonic acid secretion / phospholipid metabolic process / lipid catabolic process / negative regulation of T cell proliferation / phospholipid binding / inflammatory response / calcium ion binding / extracellular region / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Hou, S. / Xu, J. / Xu, T. / Liu, J. | ||||||
Citation | Journal: Sci Rep / Year: 2017 Title: Structural basis for functional selectivity and ligand recognition revealed by crystal structures of human secreted phospholipase A2 group IIE Authors: Hou, S. / Xu, T. / Xu, J. / Qu, L. / Xu, Y. / Chen, L. / Liu, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5wzo.cif.gz | 42.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5wzo.ent.gz | 31 KB | Display | PDB format |
PDBx/mmJSON format | 5wzo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wz/5wzo ftp://data.pdbj.org/pub/pdb/validation_reports/wz/5wzo | HTTPS FTP |
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-Related structure data
Related structure data | 5wzmC 5wzsC 5wztC 5wzuC 5wzvC 5wzwC 5y5eC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 13986.137 Da / Num. of mol.: 1 / Fragment: UNP residues 20-142 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: PLA2G2E / Plasmid: pGAPZaA / Production host: Pichia pastoris (fungus) / Strain (production host): X33 / References: UniProt: Q9NZK7, phospholipase A2 |
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-Non-polymers , 5 types, 208 molecules
#2: Chemical | ChemComp-CL / #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-B3P / | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.38 Å3/Da / Density % sol: 63.56 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / Details: 2.2M Sodium chloride, 0.1M BIS-TRIS propane pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: OXFORD DIFFRACTION ENHANCE ULTRA / Wavelength: 1.5406 Å |
Detector | Type: OXFORD RUBY CCD / Detector: CCD / Date: Aug 14, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5406 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→18.76 Å / Num. obs: 15248 / % possible obs: 98.5 % / Redundancy: 8.8 % / CC1/2: 0.994 / Rmerge(I) obs: 0.117 / Rpim(I) all: 0.042 / Rrim(I) all: 0.125 / Net I/σ(I): 16.6 |
Reflection shell | Resolution: 1.9→1.94 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.778 / CC1/2: 0.64 / Rpim(I) all: 0.402 / Rrim(I) all: 0.883 / % possible all: 85.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.9→18.76 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.898 / SU B: 3.332 / SU ML: 0.095 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.145 / ESU R Free: 0.139 Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 60.9 Å2 / Biso mean: 18.662 Å2 / Biso min: 6.04 Å2
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Refinement step | Cycle: final / Resolution: 1.9→18.76 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.949 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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