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Yorodumi- PDB-5wfd: Humanized mutant of the Chaetomium thermophilum Polycomb Repressi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5wfd | |||||||||||||||||||||
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Title | Humanized mutant of the Chaetomium thermophilum Polycomb Repressive Complex 2 bound to the inhibitor GSK126 | |||||||||||||||||||||
Components |
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Keywords | TRANSFERASE/TRANSFERASE INHIBITOR / transferase / inhibitor / complex / epigenetics / TRANSFERASE-TRANSFERASE INHIBITOR complex | |||||||||||||||||||||
Function / homology | Function and homology information histone methyltransferase activity / methylation / metal ion binding / nucleus Similarity search - Function | |||||||||||||||||||||
Biological species | Chaetomium thermophilum (fungus) | |||||||||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.654 Å | |||||||||||||||||||||
Authors | Bratkowski, M.A. / Liu, X. | |||||||||||||||||||||
Funding support | United States, 6items
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Citation | Journal: Sci Rep / Year: 2018 Title: An Evolutionarily Conserved Structural Platform for PRC2 Inhibition by a Class of Ezh2 Inhibitors. Authors: Bratkowski, M. / Yang, X. / Liu, X. | |||||||||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5wfd.cif.gz | 519.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5wfd.ent.gz | 419.4 KB | Display | PDB format |
PDBx/mmJSON format | 5wfd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wf/5wfd ftp://data.pdbj.org/pub/pdb/validation_reports/wf/5wfd | HTTPS FTP |
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-Related structure data
Related structure data | 5wf7C 5wfcC 5wg6C 5bjsS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 66021.375 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0029920 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: G0S8H7 | ||
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#2: Protein | Mass: 106801.656 Da / Num. of mol.: 1 / Mutation: P302I, R304Y, E850K, N851Y, K852M, V853C, Y855F Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0053230, CTHT_0006210 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: G0SDW4, UniProt: G0RYC6 | ||
#3: Chemical | ChemComp-ZN / #4: Chemical | ChemComp-A9G / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.74 % / Description: Plate |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 100 mM sodium malonate pH 7.0, 16% PEG 3350, 43 mM 3-cyclohexyl-1-propylphosphocholine |
-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.979 Å |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Jun 15, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.654→50 Å / Num. obs: 52188 / % possible obs: 100 % / Redundancy: 11.4 % / CC1/2: 0.882 / Rmerge(I) obs: 0.213 / Rpim(I) all: 0.066 / Net I/σ(I): 14.6 |
Reflection shell | Resolution: 2.654→2.7 Å / Redundancy: 11.1 % / Rmerge(I) obs: 3.146 / Num. unique obs: 2541 / CC1/2: 0.535 / Rpim(I) all: 0.975 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5BJS Resolution: 2.654→46.146 Å / SU ML: 0.35 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 25.5 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.654→46.146 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 22.5816 Å / Origin y: 17.9179 Å / Origin z: -35.2824 Å
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Refinement TLS group | Selection details: ALL |