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Yorodumi- PDB-5m5g: Crystal structure of the Chaetomium Thermophilum polycomb repress... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5m5g | ||||||
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Title | Crystal structure of the Chaetomium Thermophilum polycomb repressive complex 2 (PRC2) | ||||||
Components |
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Keywords | TRANSFERASE / REPRESSIVE / COMPLEX | ||||||
Function / homology | Function and homology information histone methyltransferase activity / Chromatin modifying enzymes / epigenetic regulation of gene expression / telomere organization / RNA Polymerase I Promoter Opening / Interleukin-7 signaling / Assembly of the ORC complex at the origin of replication / DNA methylation / Condensation of Prophase Chromosomes / HCMV Late Events ...histone methyltransferase activity / Chromatin modifying enzymes / epigenetic regulation of gene expression / telomere organization / RNA Polymerase I Promoter Opening / Interleukin-7 signaling / Assembly of the ORC complex at the origin of replication / DNA methylation / Condensation of Prophase Chromosomes / HCMV Late Events / Chromatin modifications during the maternal to zygotic transition (MZT) / ERCC6 (CSB) and EHMT2 (G9a) positively regulate rRNA expression / SIRT1 negatively regulates rRNA expression / PRC2 methylates histones and DNA / Defective pyroptosis / HDACs deacetylate histones / RNA Polymerase I Promoter Escape / Transcriptional regulation by small RNAs / Formation of the beta-catenin:TCF transactivating complex / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 / NoRC negatively regulates rRNA expression / B-WICH complex positively regulates rRNA expression / HDMs demethylate histones / PKMTs methylate histone lysines / RMTs methylate histone arginines / Meiotic recombination / Pre-NOTCH Transcription and Translation / nucleosome assembly / Activation of anterior HOX genes in hindbrain development during early embryogenesis / HCMV Early Events / Transcriptional regulation of granulopoiesis / structural constituent of chromatin / nucleosome / gene expression / RUNX1 regulates transcription of genes involved in differentiation of HSCs / chromatin organization / methylation / Factors involved in megakaryocyte development and platelet production / HATs acetylate histones / Senescence-Associated Secretory Phenotype (SASP) / Oxidative Stress Induced Senescence / Estrogen-dependent gene expression / cadherin binding / Amyloid fiber formation / protein heterodimerization activity / protein-containing complex / DNA binding / extracellular exosome / extracellular region / nucleoplasm / membrane / metal ion binding / nucleus Similarity search - Function | ||||||
Biological species | Chaetomium thermophilum (fungus) Chaetomium thermophilum var. thermophilum DSM 1495 (fungus) Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.27 Å | ||||||
Authors | Zhang, Y. / Justin, N. / Wilson, J. / Gamblin, S. | ||||||
Citation | Journal: Science / Year: 2016 Title: Comment on "Structural basis of histone H3K27 trimethylation by an active polycomb repressive complex 2". Authors: Zhang, Y. / Justin, N. / Wilson, J.R. / Gamblin, S.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5m5g.cif.gz | 292.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5m5g.ent.gz | 232.6 KB | Display | PDB format |
PDBx/mmJSON format | 5m5g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m5/5m5g ftp://data.pdbj.org/pub/pdb/validation_reports/m5/5m5g | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 66021.375 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0029920 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: G0S8H7 |
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#2: Protein | Mass: 106869.594 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) (fungus) Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0053230 / Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: G0SDW4 |
-Protein/peptide , 2 types, 2 molecules DE
#3: Protein/peptide | Mass: 1146.360 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P68431*PLUS |
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#4: Protein/peptide | Mass: 926.157 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chaetomium thermophilum var. thermophilum DSM 1495 (fungus) Production host: Saccharomyces cerevisiae (brewer's yeast) / References: UniProt: G0RYC6*PLUS |
-Non-polymers , 3 types, 537 molecules
#5: Chemical | ChemComp-ZN / #6: Chemical | ChemComp-SAH / | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.46 % / Description: AUTHOR USED THE SF DATA FROM ENTRY 5CH1 |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 15% PEG4000, 175mM ammonium citrate, pH 7.0 |
-Data collection
Diffraction |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 2.27→45.84 Å / Num. obs: 58693 / % possible obs: 90.69 % / Redundancy: 6.5 % / Net I/σ(I): 1.81 |
-Processing
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Refinement | Resolution: 2.27→45.84 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.929 / SU B: 6.612 / SU ML: 0.159 / Cross valid method: THROUGHOUT / ESU R: 0.346 / ESU R Free: 0.228 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 41.147 Å2
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Refinement step | Cycle: LAST / Resolution: 2.27→45.84 Å
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