Mass: 13614.342 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Lama glama (llama) / Production host: Escherichia coli (E. coli)
Mass: 18.015 Da / Num. of mol.: 209 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.56 Å3/Da / Density % sol: 52.01 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: protein stock solution was 11 mg/ml well was 10% PEG 3000, 200 mM zinc acetate, 100 mM sodium acetate
Resolution: 1.79→38.47 Å / Cor.coef. Fo:Fc: 0.947 / Cor.coef. Fo:Fc free: 0.922 / SU B: 3.251 / SU ML: 0.102 / Cross valid method: THROUGHOUT / ESU R: 0.139 / ESU R Free: 0.133 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.24249
815
3.2 %
RANDOM
Rwork
0.20425
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obs
0.20557
24347
96.41 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å