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Open data
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Basic information
Entry | Database: PDB / ID: 5v1x | ||||||
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Title | Carbon Sulfoxide lyase, Egt2 Y134F in complex with its substrate | ||||||
![]() | Hercynylcysteine sulfoxide lyase | ||||||
![]() | LYASE / C-S lyase / PLP dependent | ||||||
Function / homology | ![]() hercynylcysteine sulfoxide lyase activity (ergothioneine-forming) / Lyases; Carbon-sulfur lyases / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Irani, S. / Zhang, Y. | ||||||
![]() | ![]() Title: Snapshots of C-S Cleavage in Egt2 Reveals Substrate Specificity and Reaction Mechanism. Authors: Irani, S. / Naowarojna, N. / Tang, Y. / Kathuria, K.R. / Wang, S. / Dhembi, A. / Lee, N. / Yan, W. / Lyu, H. / Costello, C.E. / Liu, P. / Zhang, Y.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 690.1 KB | Display | ![]() |
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PDB format | ![]() | 568.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 122.8 KB | Display | |
Data in CIF | ![]() | 163.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5utsSC ![]() 5v12C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | Dimer as determined by gel filtration |
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Components
#1: Protein | Mass: 56426.262 Da / Num. of mol.: 8 / Mutation: Y134F Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987 Gene: egt-2, NCU11365 / Production host: ![]() ![]() #2: Chemical | ChemComp-FMT / #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.74 Å3/Da / Density % sol: 55.1 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 30% PEG 3350, 50mM HEPES pH 7.0, 0.2M sodium formate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 17, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.558→50 Å / Num. obs: 135254 / % possible obs: 99.4 % / Redundancy: 3.1 % / Net I/σ(I): 10.69 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5UTS Resolution: 2.558→48.806 Å / SU ML: 0.36 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 32.57 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.558→48.806 Å
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Refine LS restraints |
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LS refinement shell |
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