Mass: 30318.670 Da / Num. of mol.: 1 / Fragment: UNP residues 2461-2710 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Clostridioides difficile (bacteria) / Gene: toxA, tcdA / Plasmid: pHIS1622 / Production host: Bacillus megaterium (bacteria) References: UniProt: P16154, Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases
#2: Antibody
PA50FabLightchain
Mass: 22999.506 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#3: Antibody
PA50FabHeavychain
Mass: 23826.764 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.86 Å3/Da / Density % sol: 56.94 %
Crystal grow
Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1 M Tris pH 7.5 0.6 M calcium chloride 19% PEG 3350