NADPH-hemoprotein reductase / NADPH-hemoprotein reductase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / unspecific monooxygenase / aromatase activity / metabolic process / response to hormone / FMN binding / flavin adenine dinucleotide binding / iron ion binding ...NADPH-hemoprotein reductase / NADPH-hemoprotein reductase activity / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / unspecific monooxygenase / aromatase activity / metabolic process / response to hormone / FMN binding / flavin adenine dinucleotide binding / iron ion binding / heme binding / identical protein binding / cytosol Similarity search - Function
Mass: 54006.609 Da / Num. of mol.: 2 Mutation: A82L,F87A,P142S,T175I,A184V,S226R,H236Q,E252G,I263L,T268G,A290V,A328V,L353V,I366V,C400S,T438S,E442K Source method: isolated from a genetically manipulated source Details: Due to the extensive engineering performed to obtain this catalyst, We created a simplified name for the catalyst. The mutation details are explained in the publication Source: (gene. exp.) Bacillus megaterium (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: F2Q7T0, UniProt: P14779*PLUS
Resolution: 1.7→40 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.96 / SU B: 5.835 / SU ML: 0.099 / Cross valid method: THROUGHOUT / ESU R: 0.107 / ESU R Free: 0.106 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22324
5400
5 %
RANDOM
Rwork
0.18919
-
-
-
obs
0.19094
103324
99.63 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å