Entry Database : PDB / ID : 5tie Structure visualization Downloads & linksTitle x-ray structure of acyl-CoA thioesterase I, TesA, mutant M141L/Y145K/L146K at pH 7.5 in complex with octanoic acid ComponentsAcyl-CoA thioesterase I Details Keywords HYDROLASE / thioesterase / TesA / fatty acid / octanoic acidFunction / homology Function and homology informationFunction Domain/homology Component
arylesterase / phosphatidyl phospholipase B activity / lysophospholipase / palmitoyl-CoA hydrolase / fatty acyl-[ACP] hydrolase activity / oleoyl-[acyl-carrier-protein] hydrolase / fatty acyl-CoA hydrolase activity / lysophospholipase activity / Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases / arylesterase activity ... arylesterase / phosphatidyl phospholipase B activity / lysophospholipase / palmitoyl-CoA hydrolase / fatty acyl-[ACP] hydrolase activity / oleoyl-[acyl-carrier-protein] hydrolase / fatty acyl-CoA hydrolase activity / lysophospholipase activity / Hydrolases; Acting on peptide bonds (peptidases); Serine endopeptidases / arylesterase activity / lipid metabolic process / peptidase activity / outer membrane-bounded periplasmic space / proteolysis / identical protein binding Similarity search - Function Lipase, GDSL, active site / Lipolytic enzymes "G-D-S-L" family, serine active site. / GDSL-like Lipase/Acylhydrolase / SGNH hydrolase / SGNH hydrolase-type esterase domain / GDSL-like Lipase/Acylhydrolase family / SGNH hydrolase superfamily / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta Similarity search - Domain/homologyBiological species Escherichia coli (E. coli)Method X-RAY DIFFRACTION / FOURIER SYNTHESIS / Resolution : 1.15 Å DetailsAuthors Thoden, J.B. / Holden, H.M. / Grisewood, M.J. / Hernandez Lozada, N.J. / Gifford, N.P. / Mendez-Perez, D. / Schoenberger, H.A. / Allan, M.F. / Pfleger, B.F. / Marines, C.D. Funding support United States, 1items Details Hide detailsOrganization Grant number Country National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM115921 United States
CitationJournal : ACS Catal / Year : 2017Title : Computational Redesign of Acyl-ACP Thioesterase with Improved Selectivity toward Medium-Chain-Length Fatty Acids.Authors : Grisewood, M.J. / Hernandez Lozada, N.J. / Thoden, J.B. / Gifford, N.P. / Mendez-Perez, D. / Schoenberger, H.A. / Allan, M.F. / Floy, M.E. / Lai, R.Y. / Holden, H.M. / Pfleger, B.F. / Maranas, C.D. History Deposition Oct 2, 2016 Deposition site : RCSB / Processing site : RCSBRevision 1.0 Apr 26, 2017 Provider : repository / Type : Initial releaseRevision 1.1 Sep 27, 2017 Group : Author supporting evidence / Category : pdbx_audit_support / Item : _pdbx_audit_support.funding_organizationRevision 1.2 Feb 14, 2018 Group : Database references / Category : citation / citation_authorItem : _citation.journal_abbrev / _citation.journal_volume ... _citation.journal_abbrev / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title Revision 1.3 Dec 25, 2019 Group : Author supporting evidence / Category : pdbx_audit_support / Item : _pdbx_audit_support.funding_organizationRevision 1.4 Oct 4, 2023 Group : Data collection / Database references / Refinement descriptionCategory : chem_comp_atom / chem_comp_bond ... chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession
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