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Open data
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Basic information
Entry | Database: PDB / ID: 5t8n | ||||||
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Title | Crystal structure of Snf7 under 200 MPa | ||||||
![]() | Vacuolar-sorting protein SNF7 | ||||||
![]() | PROTEIN TRANSPORT / Snf7 / high-pressure cryocooling / resolution | ||||||
Function / homology | ![]() ESCRT III complex assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / intralumenal vesicle formation / Macroautophagy / Endosomal Sorting Complex Required For Transport (ESCRT) / ESCRT III complex / ATP export / late endosome to vacuole transport via multivesicular body sorting pathway / vesicle budding from membrane / late endosome to vacuole transport ...ESCRT III complex assembly / Sealing of the nuclear envelope (NE) by ESCRT-III / intralumenal vesicle formation / Macroautophagy / Endosomal Sorting Complex Required For Transport (ESCRT) / ESCRT III complex / ATP export / late endosome to vacuole transport via multivesicular body sorting pathway / vesicle budding from membrane / late endosome to vacuole transport / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / reticulophagy / multivesicular body / cytoplasmic side of plasma membrane / protein transport / nuclear envelope / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, Q. / Szebenyi, D. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of Snf7 under 200 MPa Authors: Huang, Q. / Szebenyi, D. #1: ![]() Title: Structural basis for activation, assembly and membrane binding of ESCRT-III Snf7 filaments. Authors: Tang, S. / Henne, W.M. / Borbat, P.P. / Buchkovich, N.J. / Freed, J.H. / Mao, Y. / Fromme, J.C. / Emr, S.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 64 KB | Display | ![]() |
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PDB format | ![]() | 46.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5fd9S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15803.133 Da / Num. of mol.: 1 / Fragment: UNP residues 12-150 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 204508 / S288c / Gene: SNF7, DID1, VPS32, YLR025W / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.7 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.5 / Details: 6% PEG20000, 110mM NaCl, 70mM Mes pH5.5 / Temp details: cold room |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Nov 26, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 6164 / % possible obs: 95.7 % / Redundancy: 3.1 % / Net I/σ(I): 19.1 |
Reflection shell | Resolution: 2.2→2.24 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.361 / Mean I/σ(I) obs: 1.5 / % possible all: 90.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5fd9 Resolution: 2.204→44.644 Å / SU ML: 0.27 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 37.21
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.204→44.644 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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