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Open data
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Basic information
Entry | Database: PDB / ID: 5t8b | ||||||
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Title | Crystal structure of lpg1496 under 350 MPa | ||||||
![]() | lpg1496 | ||||||
![]() | UNKNOWN FUNCTION / lpg1496 / high-pressure cryocooling | ||||||
Function / homology | : ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Huang, Q. / Szebenyi, D. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of lpg1496 under 350 MPa Authors: Huang, Q. / Szebenyi, D. #1: Journal: J Appl Crystallogr / Year: 2016 Title: Reduction of lattice disorder in protein crystals by high-pressure cryocooling. Authors: Huang, Q. / Gruner, S.M. / Kim, C.U. / Mao, Y. / Wu, X. / Szebenyi, D.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 79.4 KB | Display | ![]() |
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PDB format | ![]() | 61.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 415.8 KB | Display | ![]() |
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Full document | ![]() | 417.8 KB | Display | |
Data in XML | ![]() | 16.5 KB | Display | |
Data in CIF | ![]() | 25.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 34299.465 Da / Num. of mol.: 1 / Fragment: UNP residues 298-598 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.89 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.4 / Details: 12% PEG6000, 0.2M MgCl2, 0.1M Tris-HCl, pH8.4 / PH range: 8.0-8.8 / Temp details: ROOM TEMPERATURE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 25, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→50 Å / Num. obs: 47082 / % possible obs: 99.8 % / Redundancy: 24 % / Net I/σ(I): 40.6 |
Reflection shell | Resolution: 1.5→1.53 Å / Redundancy: 14 % / Rmerge(I) obs: 0.966 / Mean I/σ(I) obs: 2 / % possible all: 97.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→41.704 Å
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Refine LS restraints |
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LS refinement shell |
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