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- PDB-5t5n: Calcium-activated chloride channel bestrophin-1 (BEST1), triple m... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5t5n | ||||||
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Title | Calcium-activated chloride channel bestrophin-1 (BEST1), triple mutant: I76A, F80A, F84A; in complex with an Fab antibody fragment, chloride, and calcium | ||||||
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![]() | MEMBRANE PROTEIN / ion channel / anion / chloride / calcium-activated / eukaryotic membrane protein / CaCC | ||||||
Function / homology | ![]() Stimuli-sensing channels / chloride channel activity / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Long, S.B. / Vaisey, G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Distinct regions that control ion selectivity and calcium-dependent activation in the bestrophin ion channel. Authors: Vaisey, G. / Miller, A.N. / Long, S.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 754.3 KB | Display | ![]() |
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PDB format | ![]() | 620.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 567 KB | Display | ![]() |
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Full document | ![]() | 629.6 KB | Display | |
Data in XML | ![]() | 130 KB | Display | |
Data in CIF | ![]() | 176.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4rdqS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 5 molecules ABCDE
#1: Protein | Mass: 47420.070 Da / Num. of mol.: 5 / Mutation: I76A, F80A, F84A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Antibody , 2 types, 10 molecules FHJLNGIKMO
#2: Antibody | Mass: 23339.707 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Antibody | Mass: 23638.525 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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-Non-polymers , 5 types, 40 molecules ![](data/chem/img/CL.gif)
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![](data/chem/img/GOL.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/K.gif)
![](data/chem/img/GOL.gif)
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#4: Chemical | ChemComp-CL / #5: Chemical | ChemComp-K / #6: Chemical | ChemComp-GOL / #7: Chemical | ChemComp-CA / #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.36 Å3/Da / Density % sol: 71.77 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 6% Peg 4000 50 mM sodium acetate pH 4.0 20% glycerol 75 mM NaCl 75 mM KCl 10 mM Tris-HCl pH 7.5 0.5 mM Cymal-6-NG (Anatrace) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 1, 2014 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.033 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→38 Å / Num. obs: 145877 / % possible obs: 99.9 % / Observed criterion σ(I): -3 / Redundancy: 9.5 % / Biso Wilson estimate: 82 Å2 / Net I/σ(I): 9.7 |
Reflection shell | Resolution: 3.1→3.15 Å / Redundancy: 9.7 % / Mean I/σ(I) obs: 1.2 / CC1/2: 0.604 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ID 4RDQ Resolution: 3.1→37.952 Å / Cross valid method: THROUGHOUT / Phase error: 31.19
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Displacement parameters | Biso mean: 101.2 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→37.952 Å
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LS refinement shell | Resolution: 3.1→3.211 Å
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