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Open data
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Basic information
Entry | Database: PDB / ID: 5t56 | ||||||
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Title | [3]catenane from MccJ25 G12R/I13C/G21C lasso peptide | ||||||
![]() | (Microcin J25) x 2 | ||||||
![]() | DE NOVO PROTEIN / Catenane / Lasso Peptide | ||||||
Function / homology | killing of cells of another organism / defense response to bacterium / extracellular region / Microcin J25![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
![]() | Link, A.J. / Allen, C.D. | ||||||
![]() | ![]() Title: Self-Assembly of Catenanes from Lasso Peptides. Authors: Allen, C.D. / Link, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 233.9 KB | Display | ![]() |
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PDB format | ![]() | 200.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 475.8 KB | Display | ![]() |
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Full document | ![]() | 1.7 MB | Display | |
Data in XML | ![]() | 116.4 KB | Display | |
Data in CIF | ![]() | 108.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 1290.427 Da / Num. of mol.: 2 / Mutation: UNP residues 38-48 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein/peptide | Mass: 990.154 Da / Num. of mol.: 2 / Mutation: UNP residues 51-57 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 3.7 mg/mL [3]catenane from MccJ25 RCC lasso peptide, 95% H2O/5% D2O Label: 1H_sample / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 3.7 mg/mL / Component: [3]catenane from MccJ25 RCC lasso peptide / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength units: Not defined / Label: conditions_1 / Pressure: ambient atm / Temperature: 295 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: target function | ||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |