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- PDB-5or5: NMR structure of the complex formed by an engineered region 2 of ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5or5 | ||||||
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Title | NMR structure of the complex formed by an engineered region 2 of sigmaE in complex with GTAAAA | ||||||
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![]() | TRANSCRIPTION / promoter melting / -10 element recognition / ECF sigma factor | ||||||
Function / homology | ![]() sigma factor antagonist complex / response to osmotic stress / response to temperature stimulus / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / regulation of DNA-templated transcription initiation / sigma factor activity / DNA-templated transcription initiation / negative regulation of DNA-templated transcription ...sigma factor antagonist complex / response to osmotic stress / response to temperature stimulus / submerged biofilm formation / cellular response to cell envelope stress / cytosolic DNA-directed RNA polymerase complex / regulation of DNA-templated transcription initiation / sigma factor activity / DNA-templated transcription initiation / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / DNA binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() synthetic construct (others) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Campagne, S. / Vorholt, J.A. / Allain, F.H. | ||||||
![]() | ![]() Title: Engineered promoter selectivity of an ECF sigma factor Authors: Campagne, S. / Vorholt, J.A. / Allain, F.H. #1: ![]() Title: Structural basis for -10 promoter element melting by environmentally induced sigma factors. Authors: Campagne, S. / Marsh, M.E. / Capitani, G. / Vorholt, J.A. / Allain, F.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 452.7 KB | Display | ![]() |
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PDB format | ![]() | 375.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 10990.592 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() Strain: K12, 168 / Gene: rpoE, sigE, b2573, JW2557, sigW, ybbL, BSU01730 / Production host: ![]() ![]() |
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#2: DNA chain | Mass: 1761.288 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others) |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: 60 mM / Label: conditions / pH: 6.5 Not defined / Pressure: atmospheric atm / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 30 / Conformers submitted total number: 13 |