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- PDB-5ola: Structure of mitochondrial transcription elongation complex in co... -

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Basic information

Entry
Database: PDB / ID: 5ola
TitleStructure of mitochondrial transcription elongation complex in complex with elongation factor TEFM
Components
  • DNA (30-MER)
  • DNA (5'-D(P*AP*TP*GP*GP*TP*GP*TP*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*GP*AP*AP*C)-3')
  • DNA-directed RNA polymerase, mitochondrial
  • RNA (5'-R(P*GP*CP*GP*GP*CP*GP*CP*GP*C)-3')
  • Transcription elongation factor, mitochondrial
KeywordsTRANSCRIPTION / Elongation Factor / Mitochondria / Resolvase / RNA Polymerase
Function / homology
Function and homology information


transcription elongation by mitochondrial RNA polymerase / Mitochondrial transcription initiation / positive regulation of mitochondrial transcription / mitochondrial DNA-directed RNA polymerase complex / mitochondrial promoter sequence-specific DNA binding / transcription initiation at mitochondrial promoter / regulation of oxidative phosphorylation / Strand-asynchronous mitochondrial DNA replication / mitochondrial DNA replication / mitochondrial transcription ...transcription elongation by mitochondrial RNA polymerase / Mitochondrial transcription initiation / positive regulation of mitochondrial transcription / mitochondrial DNA-directed RNA polymerase complex / mitochondrial promoter sequence-specific DNA binding / transcription initiation at mitochondrial promoter / regulation of oxidative phosphorylation / Strand-asynchronous mitochondrial DNA replication / mitochondrial DNA replication / mitochondrial transcription / transcription elongation factor activity / DNA polymerase processivity factor activity / mitochondrial nucleoid / Transcriptional activation of mitochondrial biogenesis / DNA-directed RNA polymerase / DNA-directed RNA polymerase activity / 3'-5'-RNA exonuclease activity / sequence-specific DNA binding / mitochondrial matrix / ribonucleoprotein complex / protein-containing complex / mitochondrion / RNA binding
Similarity search - Function
Transcription elongation factor, mitochondrial / Helix-hairpin-helix motif / DNA-directed RNA polymerase, N-terminal / DNA-directed RNA polymerase, N-terminal domain superfamily / DNA-directed RNA polymerase N-terminal / Bacteriophage-type RNA polymerase family active site signature 1. / DNA-directed RNA polymerase N-terminal / DNA-directed RNA polymerase, phage-type / : / DNA-dependent RNA polymerase ...Transcription elongation factor, mitochondrial / Helix-hairpin-helix motif / DNA-directed RNA polymerase, N-terminal / DNA-directed RNA polymerase, N-terminal domain superfamily / DNA-directed RNA polymerase N-terminal / Bacteriophage-type RNA polymerase family active site signature 1. / DNA-directed RNA polymerase N-terminal / DNA-directed RNA polymerase, phage-type / : / DNA-dependent RNA polymerase / Bacteriophage-type RNA polymerase family active site signature 2. / RuvA domain 2-like / Tetratricopeptide-like helical domain superfamily / Ribonuclease H superfamily / Ribonuclease H-like superfamily / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
DNA / DNA (> 10) / RNA / RNA (> 10) / DNA-directed RNA polymerase, mitochondrial / Transcription elongation factor, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.904 Å
AuthorsHillen, H.S. / Parshin, A.V. / Agaronyan, K. / Morozov, Y. / Graber, J.J. / Chernev, A. / Schwinghammer, K. / Urlaub, H. / Anikin, M. / Cramer, P. / Temiakov, D.
Funding support United States, Germany, 5items
OrganizationGrant numberCountry
National Institutes of HealthRO1 GM104231 United States
German Research FoundationSFB860 Germany
German Research FoundationSPP1935 Germany
European Research Council693023 Germany
Volkswagen Foundation Germany
CitationJournal: Cell / Year: 2017
Title: Mechanism of Transcription Anti-termination in Human Mitochondria.
Authors: Hillen, H.S. / Parshin, A.V. / Agaronyan, K. / Morozov, Y.I. / Graber, J.J. / Chernev, A. / Schwinghammer, K. / Urlaub, H. / Anikin, M. / Cramer, P. / Temiakov, D.
History
DepositionJul 27, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 18, 2017Provider: repository / Type: Initial release
Revision 1.1Oct 25, 2017Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.2Nov 29, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Jan 17, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Transcription elongation factor, mitochondrial
B: Transcription elongation factor, mitochondrial
C: Transcription elongation factor, mitochondrial
D: Transcription elongation factor, mitochondrial
E: DNA-directed RNA polymerase, mitochondrial
N: DNA (5'-D(P*AP*TP*GP*GP*TP*GP*TP*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*GP*AP*AP*C)-3')
R: RNA (5'-R(P*GP*CP*GP*GP*CP*GP*CP*GP*C)-3')
T: DNA (30-MER)
F: DNA-directed RNA polymerase, mitochondrial
G: DNA (5'-D(P*AP*TP*GP*GP*TP*GP*TP*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*GP*AP*AP*C)-3')
H: RNA (5'-R(P*GP*CP*GP*GP*CP*GP*CP*GP*C)-3')
I: DNA (30-MER)


Theoretical massNumber of molelcules
Total (without water)407,30112
Polymers407,30112
Non-polymers00
Water00
1
A: Transcription elongation factor, mitochondrial
B: Transcription elongation factor, mitochondrial
E: DNA-directed RNA polymerase, mitochondrial
N: DNA (5'-D(P*AP*TP*GP*GP*TP*GP*TP*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*GP*AP*AP*C)-3')
R: RNA (5'-R(P*GP*CP*GP*GP*CP*GP*CP*GP*C)-3')
T: DNA (30-MER)


Theoretical massNumber of molelcules
Total (without water)203,6506
Polymers203,6506
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Transcription elongation factor, mitochondrial
D: Transcription elongation factor, mitochondrial
F: DNA-directed RNA polymerase, mitochondrial
G: DNA (5'-D(P*AP*TP*GP*GP*TP*GP*TP*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*GP*AP*AP*C)-3')
H: RNA (5'-R(P*GP*CP*GP*GP*CP*GP*CP*GP*C)-3')
I: DNA (30-MER)


Theoretical massNumber of molelcules
Total (without water)203,6506
Polymers203,6506
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)224.530, 155.550, 164.190
Angle α, β, γ (deg.)90.00, 113.58, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein
Transcription elongation factor, mitochondrial


Mass: 27471.811 Da / Num. of mol.: 4 / Fragment: UNP Residues 136-360
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TEFM, C17orf42 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL / References: UniProt: Q96QE5
#2: Protein DNA-directed RNA polymerase, mitochondrial / MtRPOL


Mass: 123326.508 Da / Num. of mol.: 2 / Fragment: UNP Residues 151-1230
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: POLRMT / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL / References: UniProt: O00411, DNA-directed RNA polymerase
#3: DNA chain DNA (5'-D(P*AP*TP*GP*GP*TP*GP*TP*AP*AP*CP*GP*CP*CP*AP*GP*AP*CP*GP*AP*AP*C)-3')


Mass: 10491.777 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#4: RNA chain RNA (5'-R(P*GP*CP*GP*GP*CP*GP*CP*GP*C)-3')


Mass: 4493.723 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#5: DNA chain DNA (30-MER)


Mass: 10394.637 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.23 Å3/Da / Density % sol: 61.87 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.5 / Details: BIS-TRIS, Ammonium Sulfate, PEG3350

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 23, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 3.9→49.16 Å / Num. obs: 46976 / % possible obs: 99.6 % / Redundancy: 13.12 % / CC1/2: 0.997 / Rmerge(I) obs: 0.258 / Net I/σ(I): 9.72
Reflection shellResolution: 3.9→4 Å / Redundancy: 12.5 % / Rmerge(I) obs: 2.187 / Mean I/σ(I) obs: 1.44 / Num. unique obs: 3366 / CC1/2: 0.545 / % possible all: 97.7

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Processing

Software
NameVersionClassification
PHENIX(1.11.1_2575: ???)refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4BOC, TEFM CTD

4boc


Resolution: 3.904→49.153 Å / SU ML: 0.59 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 31.11
RfactorNum. reflection% reflection
Rfree0.2756 2345 5 %
Rwork0.2429 --
obs0.2445 46931 99.73 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Refinement stepCycle: LAST / Resolution: 3.904→49.153 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms22644 2478 0 0 25122
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00425944
X-RAY DIFFRACTIONf_angle_d0.62435646
X-RAY DIFFRACTIONf_dihedral_angle_d15.15915338
X-RAY DIFFRACTIONf_chiral_restr0.0383992
X-RAY DIFFRACTIONf_plane_restr0.0054142
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.9042-3.98380.38661290.36092507X-RAY DIFFRACTION97
3.9838-4.07040.31691380.32062620X-RAY DIFFRACTION100
4.0704-4.1650.35771380.32322624X-RAY DIFFRACTION100
4.165-4.26910.32611380.32615X-RAY DIFFRACTION100
4.2691-4.38450.34181370.27512622X-RAY DIFFRACTION100
4.3845-4.51340.2891370.26442605X-RAY DIFFRACTION100
4.5134-4.6590.32441370.25422602X-RAY DIFFRACTION100
4.659-4.82540.27431390.24012632X-RAY DIFFRACTION100
4.8254-5.01840.28461390.25142646X-RAY DIFFRACTION100
5.0184-5.24660.25881390.25042619X-RAY DIFFRACTION100
5.2466-5.52280.30031370.26122609X-RAY DIFFRACTION100
5.5228-5.86830.28641390.26652649X-RAY DIFFRACTION100
5.8683-6.32060.35231370.27632620X-RAY DIFFRACTION100
6.3206-6.9550.30751390.24892629X-RAY DIFFRACTION100
6.955-7.95780.25991390.22512654X-RAY DIFFRACTION100
7.9578-10.0120.18971400.16522654X-RAY DIFFRACTION100
10.012-49.1570.2311430.21652679X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.01780.0044-0.0710.01780.08840.31060.96951.33411.9436-0.79820.78782.7695-1.8282-2.3079-0.00361.75770.1140.12492.2278-0.1812.329-22.541721.883368.8559
22.76120.37561.00122.94190.55122.65410.0896-0.01110.25990.0848-0.31150.08110.0519-0.05520.00011.4436-0.1363-0.08731.3132-0.1871.4467-4.297611.453774.8316
30.3219-0.8314-0.47321.32040.75722.01250.3122-0.26180.38510.0697-0.23180.4046-0.6154-0.195801.4507-0.10350.10641.7006-0.26361.6598-9.78122.58783.0251
40.25970.0401-0.40520.10620.28250.68210.64730.090.2728-0.2162-0.13980.04590.19220.0106-0.00011.6501-0.0522-0.26331.13670.07791.34312.2935-13.625962.1645
50.99380.06120.61670.22470.14330.2438-0.1370.7250.1644-1.64020.5477-0.5243-0.2488-0.0750.00022.03250.1636-0.4751.6476-0.20171.4991-9.39-3.31451.1355
61.3479-1.22930.79221.0768-0.80021.59340.05040.4553-0.3232-0.742-0.09680.41140.2066-0.110701.3827-0.0052-0.18071.3205-0.15071.3899-4.65990.15561.0722
70.1297-0.24630.23780.3762-0.32160.2814-0.6475-0.3202-0.548-0.79010.054-0.3973-0.61932.01160.00091.9986-0.20130.07541.7976-0.02841.11887.9994-7.724848.4378
80.20080.20040.18730.13150.18230.20510.67961.44930.43240.00830.3913-1.44020.55450.76130.00112.45420.2695-0.45651.9397-0.03921.7145-1.3834-19.750438.8787
91.00220.6188-0.84970.8436-0.69740.5967-0.17691.7516-0.2121-0.84540.44930.796-0.68930.1631-0.00072.25970.0255-0.43241.6037-0.04541.1687-5.9199-12.826545.1571
100.50630.3296-0.52580.2264-0.28290.48260.1802-0.7845-0.54361.53570.1847-2.86321.62191.55570.00261.63030.038-0.28091.91710.09991.806-20.2109-27.215529.496
112.9520.36740.04973.3698-2.46353.15540.2559-0.2887-0.11950.3306-0.17670.1010.1859-0.0898-01.2023-0.112-0.10441.2649-0.10661.2123-38.3057-22.750624.8784
120.84530.7049-0.59910.5447-0.41450.30940.29470.78191.3119-0.4457-0.64630.8071-1.0715-0.27540.00061.20090.1816-0.09681.60990.05691.6649-32.66697.92936.8039
130.98720.460.290.37110.19790.5935-0.79570.40340.7351-0.51350.87820.38770.16280.01490.00021.0237-0.1220.25211.53630.47341.9597-16.49555.27527.1466
140.42150.3749-0.0180.5734-0.16040.0639-0.27982.0121-0.6482-0.81390.7593-2.22941.18192.59280.0081.16610.2601-0.09491.8725-0.06342.2241-15.7204-15.15738.5837
151.9692-0.6933-0.65911.9228-0.86861.64060.02980.96580.0287-0.1786-0.102-0.60990.28140.5591-0.00041.04150.0846-0.13091.42230.04431.2173-25.3205-7.57559.0369
160.0188-0.03070.0370.06430.04460.0795-0.44170.4070.51630.89551.21881.7152-0.437-0.37580.00031.11730.0017-0.1711.3289-0.02181.4045-34.31292.946419.6543
170.5149-0.29830.03570.55170.33560.3107-1.0581.0608-0.2315-0.9740.0693-0.37220.30510.8324-0.00661.05680.1820.14021.81270.11261.4611-22.24473.7369-6.8427
180.09350.24750.14772.17080.18080.20540.46150.75980.3329-0.6283-0.69581.7747-0.1431.1908-0.04551.32710.24180.41492.45390.97271.8412-12.847718.6061-3.1446
190.2826-0.24950.22650.1346-0.08520.12880.01071.9712-0.10140.6438-0.6556-1.22921.13882.27950.0021.47180.08520.28872.71640.49982.2765-6.1974.8141.544
200.26140.06420.37730.2780.17950.4287-0.8505-0.7659-0.995-0.09720.5686-0.30560.27440.59180.00010.90010.09030.27061.50610.16141.3757-22.958.17520.505
210.4948-0.1776-1.22641.7150.27381.46040.0057-0.9676-0.15151.49670.2334-0.21691.09540.45610.00012.4492-0.1449-0.47542.25930.49921.946541.671622.7357105.6777
225.4803-0.10710.16420.66310.05381.16390.2954-0.71610.27530.3987-0.33690.16820.009-0.0877-0.00011.4794-0.1285-0.00141.2383-0.00051.391427.632240.490683.6731
231.3315-0.3682-0.4171.30460.2861.513-0.2496-0.2302-0.63950.23720.14070.42030.071-0.2886-01.4411-0.1417-0.01971.22390.05081.507530.845439.089566.7057
240.96250.27920.01311.95290.12110.5446-0.23530.5858-0.4212-0.44310.1111-0.26950.2643-0.0796-01.5961-0.15370.07391.4826-0.06991.634543.12726.72251.0706
250.66270.1979-0.70140.0509-0.05780.3993-0.33210.5206-0.5045-0.97070.20671.12990.0031-0.0801-02.7888-0.6962-0.72092.46980.15482.78927.263222.13155.6213
261.5918-1.31672.16543.9894-2.21162.86981.6838-0.1614-1.67110.54141.08850.4099-0.6574-1.36210.13922.3545-0.10821.0973.08550.57784.300821.7537-12.543268.0712
270.4424-0.3666-0.38970.16450.28180.1494-1.24630.1702-0.93371.33350.21791.02381.56470.47220.00212.298-0.06790.21271.8172-0.02371.998124.901329.150558.3116
281.48380.1001-0.5521-0.1403-0.3911-0.6785-0.49830.0796-0.3639-1.73170.3459-0.1223-0.1187-0.24990.00211.9588-0.26370.04721.7950.01412.208526.91527.62761.6418
290.27430.1604-0.08390.26250.03890.05691.30140.5918-0.5529-2.598-0.23061.99090.96960.2577-0.0051.80830.064-0.41772.2453-0.34133.33614.208332.3660.6359
300.0142-0.0028-0.02630.0723-0.06060.08331.5173-0.83280.42331.5433-0.05110.8015-0.1204-0.4407-0.00142.71730.175-0.3893.5655-0.29392.7731-12.679243.68656.0808
310.1696-0.250.79662.0152-0.35021.01960.2049-0.34340.5490.57650.20150.33890.2737-0.8599-0.00011.28650.0382-0.01542.1812-0.39821.9404-91.0236-29.63328.2979
323.4276-0.12851.95240.2121-0.54531.48440.2363-0.45710.03820.0946-0.2560.01660.3843-0.2950.00011.1453-0.1715-0.04961.3283-0.21491.4169-65.5366-46.79612.5035
330.1080.2699-0.12231.0489-0.0161.56160.00810.1534-0.1614-0.05170.0162-0.0939-0.12170.050901.25840.001-0.01791.362-0.23581.3769-61.225-42.4315-10.5612
340.9741-0.17880.16131.8606-0.32470.5930.01450.25740.1773-0.37510.0342-0.4594-0.52260.535501.6724-0.22820.08771.6076-0.21721.5859-50.6021-33.1473-30.0254
350.025-0.11320.06220.7651-0.52690.70820.6024-0.40010.04910.91331.37910.0349-0.95780.97130.07592.3703-0.5661-0.19643.1572-1.14864.1687-23.2304-38.09046.5278
360.74250.17270.33880.4487-0.14940.1969-1.434-1.14591.33391.18280.6804-1.1013-0.62940.3302-0.00013.6561-0.0649-0.2532.4608-0.26692.6615-47.911.1822-10.3133
370.33080.17070.34920.4443-0.42040.51110.02371.16610.49561.7077-0.3194-0.1481-3.0365-0.6201-0.00122.1811-0.19740.08742.0324-0.30362.0139-44.805-34.5616-12.8777
380.25070.08881.50660.35470.20652.0665-0.09260.588-0.4204-0.80740.1241-1.1964-0.82840.6571-0.00032.1134-0.24070.06451.7777-0.00731.8447-48.8071-13.2397-11.9648
393.15260.295-0.46830.027-0.06950.1350.9323-0.80622.51110.6927-0.1824-0.26860.50060.75740.38252.5044-0.0037-0.88723.01210.04782.0307-24.22-42.90498.6256
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 146 through 168 )
2X-RAY DIFFRACTION2chain 'A' and (resid 169 through 261 )
3X-RAY DIFFRACTION3chain 'A' and (resid 262 through 357 )
4X-RAY DIFFRACTION4chain 'B' and (resid 151 through 175 )
5X-RAY DIFFRACTION5chain 'B' and (resid 176 through 208 )
6X-RAY DIFFRACTION6chain 'B' and (resid 209 through 272 )
7X-RAY DIFFRACTION7chain 'B' and (resid 273 through 296 )
8X-RAY DIFFRACTION8chain 'B' and (resid 297 through 315 )
9X-RAY DIFFRACTION9chain 'B' and (resid 316 through 357 )
10X-RAY DIFFRACTION10chain 'C' and (resid 146 through 168 )
11X-RAY DIFFRACTION11chain 'C' and (resid 169 through 357 )
12X-RAY DIFFRACTION12chain 'D' and (resid 151 through 175 )
13X-RAY DIFFRACTION13chain 'D' and (resid 176 through 196 )
14X-RAY DIFFRACTION14chain 'D' and (resid 197 through 208 )
15X-RAY DIFFRACTION15chain 'D' and (resid 209 through 261 )
16X-RAY DIFFRACTION16chain 'D' and (resid 262 through 272 )
17X-RAY DIFFRACTION17chain 'D' and (resid 273 through 304 )
18X-RAY DIFFRACTION18chain 'D' and (resid 305 through 318 )
19X-RAY DIFFRACTION19chain 'D' and (resid 319 through 337 )
20X-RAY DIFFRACTION20chain 'D' and (resid 338 through 357 )
21X-RAY DIFFRACTION21chain 'E' and (resid 218 through 378 )
22X-RAY DIFFRACTION22chain 'E' and (resid 379 through 620 )
23X-RAY DIFFRACTION23chain 'E' and (resid 621 through 751 )
24X-RAY DIFFRACTION24chain 'E' and (resid 752 through 1230 )
25X-RAY DIFFRACTION25chain 'N' and (resid -25 through -3 )
26X-RAY DIFFRACTION26chain 'N' and (resid -2 through 3 )
27X-RAY DIFFRACTION27chain 'R' and (resid 1 through 9 )
28X-RAY DIFFRACTION28chain 'T' and (resid -12 through 7 )
29X-RAY DIFFRACTION29chain 'T' and (resid 8 through 12 )
30X-RAY DIFFRACTION30chain 'T' and (resid 13 through 17 )
31X-RAY DIFFRACTION31chain 'F' and (resid 218 through 378 )
32X-RAY DIFFRACTION32chain 'F' and (resid 379 through 614 )
33X-RAY DIFFRACTION33chain 'F' and (resid 615 through 722 )
34X-RAY DIFFRACTION34chain 'F' and (resid 723 through 1230 )
35X-RAY DIFFRACTION35chain 'G' and (resid -25 through -8 )
36X-RAY DIFFRACTION36chain 'G' and (resid -7 through 3 )
37X-RAY DIFFRACTION37chain 'H' and (resid 1 through 9 )
38X-RAY DIFFRACTION38chain 'I' and (resid -12 through 7 )
39X-RAY DIFFRACTION39chain 'I' and (resid 8 through 17 )

+
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