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- PDB-5ohm: K33-specific affimer bound to K33 diUb -

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Basic information

Entry
Database: PDB / ID: 5ohm
TitleK33-specific affimer bound to K33 diUb
Components
  • K33-specific affimer
  • Polyubiquitin-C
KeywordsSIGNALING PROTEIN / ubiquitin / cystatin
Function / homology
Function and homology information


Maturation of protein E / Maturation of protein E / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / IRAK1 recruits IKK complex ...Maturation of protein E / Maturation of protein E / ER Quality Control Compartment (ERQC) / Myoclonic epilepsy of Lafora / FLT3 signaling by CBL mutants / Prevention of phagosomal-lysosomal fusion / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / Glycogen synthesis / IRAK1 recruits IKK complex / IRAK1 recruits IKK complex upon TLR7/8 or 9 stimulation / Membrane binding and targetting of GAG proteins / Endosomal Sorting Complex Required For Transport (ESCRT) / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / PTK6 Regulates RTKs and Their Effectors AKT1 and DOK1 / Negative regulation of FLT3 / Constitutive Signaling by NOTCH1 HD Domain Mutants / Regulation of FZD by ubiquitination / TICAM1,TRAF6-dependent induction of TAK1 complex / NOTCH2 Activation and Transmission of Signal to the Nucleus / TICAM1-dependent activation of IRF3/IRF7 / APC/C:Cdc20 mediated degradation of Cyclin B / p75NTR recruits signalling complexes / Downregulation of ERBB4 signaling / APC-Cdc20 mediated degradation of Nek2A / PINK1-PRKN Mediated Mitophagy / TRAF6-mediated induction of TAK1 complex within TLR4 complex / TRAF6 mediated IRF7 activation in TLR7/8 or 9 signaling / Pexophagy / Regulation of innate immune responses to cytosolic DNA / InlA-mediated entry of Listeria monocytogenes into host cells / VLDLR internalisation and degradation / Downregulation of ERBB2:ERBB3 signaling / NF-kB is activated and signals survival / NRIF signals cell death from the nucleus / Regulation of PTEN localization / Activated NOTCH1 Transmits Signal to the Nucleus / Regulation of BACH1 activity / Translesion synthesis by REV1 / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Translesion synthesis by POLK / MAP3K8 (TPL2)-dependent MAPK1/3 activation / TICAM1, RIP1-mediated IKK complex recruitment / Downregulation of TGF-beta receptor signaling / Activation of IRF3, IRF7 mediated by TBK1, IKKε (IKBKE) / Translesion synthesis by POLI / Gap-filling DNA repair synthesis and ligation in GG-NER / Josephin domain DUBs / Regulation of activated PAK-2p34 by proteasome mediated degradation / InlB-mediated entry of Listeria monocytogenes into host cell / IKK complex recruitment mediated by RIP1 / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / N-glycan trimming in the ER and Calnexin/Calreticulin cycle / Autodegradation of Cdh1 by Cdh1:APC/C / TNFR1-induced NF-kappa-B signaling pathway / APC/C:Cdc20 mediated degradation of Securin / Asymmetric localization of PCP proteins / TCF dependent signaling in response to WNT / SCF-beta-TrCP mediated degradation of Emi1 / NIK-->noncanonical NF-kB signaling / Ubiquitin-dependent degradation of Cyclin D / Regulation of NF-kappa B signaling / AUF1 (hnRNP D0) binds and destabilizes mRNA / TNFR2 non-canonical NF-kB pathway / activated TAK1 mediates p38 MAPK activation / Assembly of the pre-replicative complex / Vpu mediated degradation of CD4 / NOTCH3 Activation and Transmission of Signal to the Nucleus / Negative regulators of DDX58/IFIH1 signaling / Deactivation of the beta-catenin transactivating complex / Degradation of DVL / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Regulation of signaling by CBL / Dectin-1 mediated noncanonical NF-kB signaling / Cdc20:Phospho-APC/C mediated degradation of Cyclin A / Fanconi Anemia Pathway / Hh mutants are degraded by ERAD / Recognition of DNA damage by PCNA-containing replication complex / Negative regulation of FGFR3 signaling / Peroxisomal protein import / Degradation of AXIN / Degradation of GLI1 by the proteasome / Downregulation of SMAD2/3:SMAD4 transcriptional activity / Activation of NF-kappaB in B cells / Regulation of TNFR1 signaling / Termination of translesion DNA synthesis / Negative regulation of FGFR2 signaling / Hedgehog ligand biogenesis / Defective CFTR causes cystic fibrosis / Stabilization of p53 / EGFR downregulation / Negative regulation of FGFR4 signaling / Negative regulation of NOTCH4 signaling / Iron uptake and transport / GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2 / Negative regulation of FGFR1 signaling / G2/M Checkpoints / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / Vif-mediated degradation of APOBEC3G
Similarity search - Function
Ubiquitin domain signature. / Ubiquitin conserved site / Ubiquitin domain / Ubiquitin family / Ubiquitin homologues / Ubiquitin domain profile. / Ubiquitin-like domain / Ubiquitin-like domain superfamily
Similarity search - Domain/homology
Polyubiquitin-C / Polyubiquitin-C
Similarity search - Component
Biological speciesHomo sapiens (human)
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.8 Å
AuthorsMichel, M.A. / Komander, D.
CitationJournal: Mol. Cell / Year: 2017
Title: Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked Ubiquitin Signaling.
Authors: Michel, M.A. / Swatek, K.N. / Hospenthal, M.K. / Komander, D.
History
DepositionJul 17, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 4, 2017Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2017Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Jan 2, 2019Group: Data collection / Database references / Source and taxonomy
Category: entity_src_gen / struct_ref ...entity_src_gen / struct_ref / struct_ref_seq / struct_ref_seq_dif
Item: _entity_src_gen.gene_src_common_name / _entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id ..._entity_src_gen.gene_src_common_name / _entity_src_gen.pdbx_gene_src_ncbi_taxonomy_id / _entity_src_gen.pdbx_gene_src_scientific_name / _struct_ref.db_code / _struct_ref.pdbx_db_accession / _struct_ref_seq.pdbx_db_accession / _struct_ref_seq_dif.pdbx_seq_db_accession_code
Revision 1.3Jan 17, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Polyubiquitin-C
C: Polyubiquitin-C
E: Polyubiquitin-C
G: Polyubiquitin-C
I: Polyubiquitin-C
K: Polyubiquitin-C
D: K33-specific affimer
H: K33-specific affimer
L: K33-specific affimer
B: K33-specific affimer
F: K33-specific affimer
J: K33-specific affimer
hetero molecules


Theoretical massNumber of molelcules
Total (without water)134,54414
Polymers131,48412
Non-polymers3,0602
Water00
1
A: Polyubiquitin-C
C: Polyubiquitin-C
D: K33-specific affimer
B: K33-specific affimer


Theoretical massNumber of molelcules
Total (without water)43,8284
Polymers43,8284
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4760 Å2
ΔGint-36 kcal/mol
Surface area14980 Å2
MethodPISA
2
E: Polyubiquitin-C
G: Polyubiquitin-C
H: K33-specific affimer
F: K33-specific affimer
hetero molecules


Theoretical massNumber of molelcules
Total (without water)45,3585
Polymers43,8284
Non-polymers1,5301
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4870 Å2
ΔGint-36 kcal/mol
Surface area15030 Å2
MethodPISA
3
I: Polyubiquitin-C
K: Polyubiquitin-C
L: K33-specific affimer
J: K33-specific affimer
hetero molecules


Theoretical massNumber of molelcules
Total (without water)45,3585
Polymers43,8284
Non-polymers1,5301
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4760 Å2
ΔGint-38 kcal/mol
Surface area14950 Å2
MethodPISA
Unit cell
Length a, b, c (Å)55.866, 149.604, 73.788
Angle α, β, γ (deg.)90.00, 110.44, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
Polyubiquitin-C


Mass: 8675.962 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: UBC / Production host: Escherichia coli (E. coli) / References: UniProt: P0CG48, UniProt: P0CG64*PLUS
#2: Protein
K33-specific affimer


Mass: 13238.074 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: Escherichia coli (E. coli)
#3: Chemical ChemComp-15P / POLYETHYLENE GLYCOL (N=34) / PEG 1500


Mass: 1529.829 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C69H140O35 / Comment: precipitant*YM

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.2 Å3/Da / Density % sol: 44.03 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop / pH: 7.4 / Details: 20% PEG3350 0.2M potassium thiocyanate

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Data collection

DiffractionMean temperature: 110 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.91741 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 12, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.91741 Å / Relative weight: 1
ReflectionResolution: 3.8→48.46 Å / Num. obs: 10962 / % possible obs: 97.9 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.237 / Net I/σ(I): 3.8
Reflection shellResolution: 3.8→4.25 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.779 / Mean I/σ(I) obs: 1.5 / CC1/2: 0.665 / % possible all: 99.2

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Processing

Software
NameVersionClassification
PHENIX(1.10.1_2155: ???)refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5OHV

5ohv


Resolution: 3.8→48.46 Å / SU ML: 0.59 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 33.95 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2825 499 4.55 %
Rwork0.2328 --
obs0.2352 10962 97.59 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 3.8→48.46 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7123 0 36 0 7159
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0037285
X-RAY DIFFRACTIONf_angle_d0.6559931
X-RAY DIFFRACTIONf_dihedral_angle_d11.9484300
X-RAY DIFFRACTIONf_chiral_restr0.0441217
X-RAY DIFFRACTIONf_plane_restr0.0041250
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.8-4.18230.35731120.29452648X-RAY DIFFRACTION99
4.1823-4.7870.25481420.2122575X-RAY DIFFRACTION97
4.787-6.02930.28121150.24152571X-RAY DIFFRACTION96
6.0293-48.46420.27261300.21222670X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.41641.0558-0.11372.50950.11141.28890.6216-0.4190.30660.4217-0.118-0.325-0.66560.50760.080.725-0.66190.01240.6046-0.35440.125-32.9672-23.549119.3506
22.8640.7627-0.43892.49940.81620.7420.718-0.48370.60940.26090.08920.3287-0.0572-0.09290.71441.6293-1.0776-0.10930.7810.48331.0057-32.4043-25.055322.5693
30.31020.15620.12070.8490.26640.10180.1554-0.58030.01541.04810.4258-0.37220.27280.3437-0.35172.2702-0.5804-0.0371.6149-0.57380.6106-33.5617-30.672328.3508
40.61110.19850.14151.25250.76161.81570.637-0.4031-0.71621.22420.1867-0.24460.96080.2986-0.08741.6716-0.2616-0.66771.0418-0.04220.4103-35.673-36.787322.9399
54.91790.5115-0.9099.85994.37664.88450.155-0.0316-0.89930.25130.0146-0.77850.89961.7516-0.36510.51460.29150.45360.9824-0.68171.0807-26.1783-32.971512.964
63.8746-0.36951.294.17134.40515.39480.5696-1.479-0.84131.79870.0947-1.07191.47811.5997-0.2991.5883-0.0335-0.96291.40330.43711.596-22.3112-33.119421.3759
73.4052-3.56221.51549.48262.69093.85420.651-0.35571.11640.4348-0.3584-0.7589-0.68791.1266-0.17311.1635-1.06510.13551.23190.39090.6301-24.7102-22.024717.194
81.4045-0.3649-1.24470.09480.31741.09890.8703-0.2053-0.0794-0.15040.5679-0.03020.7833-0.1569-0.48541.2997-0.2892-0.36580.66610.10910.8444-36.2675-35.681118.2167
90.49990.73230.06421.523-0.57690.8339-0.3004-0.35810.20081.603-0.52690.85690.9185-0.30790.19230.8093-0.95720.48560.5747-0.08240.9386-52.5287-46.322920.129
106.5665-3.5619-1.09225.2476-1.29181.25170.3905-1.3423-0.7310.8113-0.2741-0.1352-0.16510.1846-0.16671.034-0.64410.03651.25180.13530.6214-52.0526-40.696327.856
110.80270.143-0.94710.3511-0.17891.11440.122-0.27170.10690.062-0.13210.0002-0.12860.24530.0071.0994-0.42011.04581.23520.18310.4922-49.8155-34.591923.0831
124.21891.085-1.44664.8045-3.13674.8343-0.0755-0.14880.03290.2994-0.12590.532-0.0721-0.18740.12081.9520.37650.70681.1077-0.45760.846-59.9717-35.817617.2052
131.54420.2356-0.4460.6271-0.731.18110.23810.15070.76170.6403-0.64270.07040.1359-0.296-0.2630.4484-1.14450.705-0.10470.15461.1929-56.2024-40.193217.8244
141.2794-1.28672.85925.3855-1.10617.16430.18090.32010.0814-0.2104-0.6060.8648-1.0493-1.53920.05070.54380.52730.33250.7394-0.02871.6922-45.0571-0.4505-10.4882
150.0689-0.0420.31940.75050.34012.04-0.18950.1206-0.0950.3312-0.12050.44990.3692-0.833-0.02280.92950.08711.40471.01190.32621.5135-39.85571.4903-9.6066
161.6336-0.9614-2.11860.57221.24272.7517-0.07170.76890.328-0.0733-0.06860.53760.3376-0.60360.33721.09420.26090.35631.7094-0.35571.7108-54.68152.5962-17.6237
174.36552.7334-3.35611.819-1.42986.85310.066-1.0567-0.29250.77050.72070.32430.4323-0.4446-0.50181.06230.35810.25560.94550.5311.3799-43.97677.5495-18.336
180.4650.6651-0.32613.21571.2071.47280.08290.2195-0.1594-0.2786-0.10710.0720.88960.2575-0.0241.62391.0158-0.69520.8762-0.16020.869-37.87933.7149-22.1004
194.9687-1.0889-5.38360.44110.80026.53420.27710.6702-1.2274-0.49810.14630.38781.25210.7981-0.52120.85580.4951-0.29830.8486-0.61462.1594-43.5558-9.1134-20.0496
202.73961.8761-4.56131.2844-3.12497.60010.99150.94580.2662-0.00220.48590.5231-0.4907-0.6064-1.39291.15130.7351-0.41582.09030.31912.4908-47.9452-2.3802-26.3365
210.7437-0.6941.29720.6857-1.24872.29680.97691.416-0.5158-0.10450.40360.91250.0127-1.1032-1.46171.06070.3415-0.40341.75480.44462.4893-52.3149-4.6461-21.1803
224.16494.2454-1.83314.3291-1.85080.93781.26041.3029-0.5856-0.77550.3471.5138-0.3675-0.7447-1.26931.07840.18540.1050.699-0.26321.142-51.4035-5.7662-11.9405
230.7848-0.05650.43010.3927-0.7231.55790.54680.70410.8036-1.1407-0.7522-0.758-0.31550.2764-0.24531.55070.62950.27440.8233-0.16111.2715-35.6340.0809-20.7783
240.35610.2988-0.47110.8191-1.17491.7333-0.148-0.12010.1629-0.4083-0.6412-0.8681-0.38271.0904-0.42442.2381-0.72661.98361.0889-0.23092.2929-18.4647.9442-23.1345
250.17550.10020.30072.03110.13730.5171-0.3264-0.3770.51610.23620.3412-0.4838-0.8215-0.4540.11191.690.41690.60450.91150.01122.205-24.481714.5727-19.5794
262.03531.2259-0.88340.8039-0.21212.16711.0005-0.48680.23681.1267-0.0460.971-0.1775-0.3802-0.23812.053-0.33340.81350.7744-0.10562.2462-23.91217.7579-13.6077
270.6423-0.7144-0.16280.79430.18150.04150.0874-0.20511.0910.2362-0.25670.3368-0.59090.09570.09981.6994-0.8126-0.28190.94390.02482.3734-18.118413.3099-10.3043
286.3876-0.38765.00985.6865-3.74186.0176-1.18271.64651.05790.2484-0.3655-0.2421-0.70720.69661.47630.5546-0.09330.50841.1453-0.06211.949-9.90419.4919-18.1098
291.043-0.3352-0.10376.92890.31623.70150.3039-0.51410.2531-0.2428-0.4586-0.3542-0.2760.6474-0.80711.2196-0.06360.73850.96590.4651.6928-25.89354.6493-14.4041
301.3067-0.9342-0.88854.1478-3.19394.8158-0.091-0.0125-0.24662.0951-0.3857-0.00890.94031.58150.41722.39780.126-1.16450.702-0.04221.6673-48.9759-2.040826.0632
310.27450.2001-0.57830.35330.05152.25580.71640.42110.472-0.88340.5094-0.5218-1.197-0.7281-0.93473.14470.1724-0.36950.9540.01131.2007-55.2933-7.936923.5206
321.989-2.66531.31523.5797-1.62797.36590.76960.1523-0.47770.5464-1.2039-0.31330.57880.27570.50151.8616-0.14030.13050.250.09152.5479-54.2306-2.074916.3684
330.31530.39380.88092.37140.91172.47910.3610.6873-0.6234-0.4162-0.4555-0.42340.94711.17640.1722.40070.5741-0.12980.8977-0.13091.8169-47.643-7.459512.3461
345.33610.68782.85831.7358-2.03365.04220.19910.29830.0699-0.3803-0.1215-0.22610.25841.16410.20291.16770.1572-0.28671.56630.281.0811-40.8005-3.763920.0858
351.1953-0.51-1.88221.8342-0.06713.43450.04230.3267-0.21770.1788-0.1981-0.23840.29351.0817-0.40920.9717-0.1604-1.07240.86650.19421.0099-55.48312.086318.0205
360.5048-0.1071-1.12270.70010.5532.62770.5939-0.2942-0.2746-0.1246-0.01511.11951.2346-1.33170.02641.4723-0.7069-0.82810.94240.04891.5998-76.68785.915811.661
370.83290.18460.82210.11310.17990.82840.248-0.6461-0.09420.5925-0.13171.21151.3146-0.935-0.45141.1804-0.2471-0.02981.08750.39781.5206-74.06044.353715.0723
380.8005-0.8954-0.8761.82860.33911.45070.4348-0.08250.1454-0.29990.0289-0.2481-0.23750.0273-0.13891.6898-0.9288-0.61111.32250.76161.3676-74.2505-1.947420.6992
390.7648-0.85710.06091.2618-0.70583.09960.466-0.9032-0.94120.59540.1854-0.92990.5906-0.2879-0.3021.1015-0.5167-0.03481.96660.42491.8052-70.25136.414323.6621
403.95090.69630.34412.6954-0.20850.6080.0966-0.3501-0.27440.06750.1430.38560.2853-0.6097-0.14081.198-1.2208-0.36631.98650.22851.1553-78.21548.167527.7696
412.63173.3742-1.39565.9019-0.63384.04680.7374-1.06810.08311.2274-0.10820.50270.6943-1.5907-0.80440.6916-0.44020.19311.34020.01411.403-75.69459.715320.0974
427.1655-2.28564.06982.4193-0.18523.28820.20990.18870.64680.13890.24510.04010.34670.66220.28520.6249-0.16780.18890.5264-0.05560.8329-49.0747-23.0488-1.6142
434.2359-1.4331-0.08818.3225-0.49176.83590.45750.35031.1350.1008-0.83380.0383-0.5104-1.0711-0.10280.80650.09060.04990.77050.12560.7248-50.2188-19.1657-1.1683
446.33060.04750.00875.98890.78555.6459-0.14530.45941.14960.15240.27930.0241-0.314-0.29490.14430.4426-0.3972-0.32090.4603-0.12420.4971-45.3035-23.61753.2478
453.61710.026-0.11424.54860.79981.5710.3535-0.28210.60010.16620.403-0.0871-0.07740.08580.53970.0874-0.35620.54950.36180.18961.6461-46.143-26.40725.2391
463.5334-2.18690.52192.4273-1.12734.08430.66011.0364-0.3051-0.35570.3903-1.6327-0.20080.9226-0.20520.8314-0.03760.32690.878-0.10840.8476-19.0373-12.8362-1.7668
471.3231-1.63242.4364.43270.17878.67570.35160.27480.49220.88380.0094-1.03580.19441.8625-0.2190.7705-0.1060.09550.6436-0.15991.9052-14.3968-14.89244.5963
482.01560.2043-0.93581.1684-1.52762.72240.10181.00820.5152-0.12360.63960.449-0.053-0.7160.03250.7105-0.79890.5450.5909-0.2011.6534-37.0324-13.4515-1.8194
492.72582.1761.43333.2437-0.02815.866-0.5440.92790.2381-0.21410.6478-0.16140.12180.1386-0.33070.3334-0.27530.29990.6548-0.02721.3581-27.7768-13.34771.4363
502.92340.4281.85423.93851.89973.3257-0.2850.58280.28890.4120.2183-0.65890.05630.52040.07430.4474-0.18840.54910.8322-0.04231.3417-22.7161-11.06142.95
515.9208-0.57915.19140.6326-0.37044.5378-0.08630.329-0.4101-0.7038-0.2019-0.30210.4008-0.1361-0.07350.5657-0.5593-0.11490.7674-0.16160.5918-28.211-9.0455-7.9158
522.9569-0.76752.53222.16520.23223.80760.3809-0.3195-0.1772-0.13750.36780.48950.86150.3451-1.07450.9223-0.4504-0.87961.35280.1370.8363-59.588328.040126.1555
538.0178-1.7274-3.36288.3381-1.89822.348-0.5435-0.2635-0.76051.1425-0.88520.0514-0.31540.30211.39611.93080.0499-0.55550.96480.13431.4837-63.166134.409929.7128
542.24270.63360.58942.0680.22882.8193-0.3441-0.51840.33641.0817-0.186-0.4872-0.54660.00220.48171.6862-0.1141-0.02621.57080.38520.9987-45.998317.15828.0363
550.69480.14550.62070.77920.32051.8995-0.0383-0.90690.60490.8469-0.033-0.0879-0.5941-0.19291.65542.21240.0842-1.16671.3799-0.32460.8112-56.142425.636330.4406
561.2470.55370.7853.24251.12742.7297-0.2434-1.07190.42050.9799-0.33-0.4674-0.5228-0.34490.38281.1094-0.1471-0.81991.0358-0.34751.2496-55.280215.859921.6149
573.4559-2.0535-1.92987.92721.52664.54680.67440.11920.4485-1.15780.0047-0.7989-0.72850.3682-0.10551.0368-0.21620.49240.2209-0.33430.7201-31.5633-43.249-3.7352
585.1159-0.3128-1.61124.30621.16173.08580.2799-0.0942-0.19830.2785-0.0671-0.2241-0.12590.13250.41080.4574-0.2037-0.05670.63370.17340.871-35.634-48.7158-3.0313
597.71811.67960.80188.76510.50655.88280.45910.9336-1.0472-0.1426-0.654-0.40480.38770.3536-0.11630.6798-0.01150.03310.6689-0.14360.8819-35.0265-48.4809-0.4601
600.0003-0.04240.0235.90881.79436.35450.1696-1.3596-1.0920.70350.444-0.0857-0.15460.2772-0.680.3823-0.2040.07740.42790.26671.097-39.5235-41.20225.3311
615.01241.72580.59029.03540.77756.3046-0.1740.7604-1.3685-1.24430.5769-1.31641.09780.7586-0.05071.45960.1340.19190.9872-0.35131.6553-25.2992-20.9288-24.5119
621.11-0.4566-0.01563.22540.25212.7364-0.01661.0883-1.026-1.2001-0.3658-1.43020.11160.9332-0.68051.36750.14890.81341.0233-0.35690.9206-24.3033-16.3125-25.1106
631.49581.9517-1.57423.0134-0.30848.80640.1407-0.6273-0.41450.377-0.18370.0845-1.29330.2491-0.36570.29340.0160.05060.46910.04621.3858-51.573919.65142.0575
640.01010.13380.24984.0234-0.99954.96030.5663-0.1577-0.2615-0.04930.1491-0.709-0.5347-0.59770.24360.6845-0.2502-0.23960.26240.40271.3504-54.508817.41421.3727
654.4617-1.8882-4.13377.22092.97848.368-0.0626-0.30290.15130.9-0.10190.22681.25640.30880.31080.6851-0.0792-0.280.58830.12821.2669-55.920114.42277.934
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 7 )
2X-RAY DIFFRACTION2chain 'A' and (resid 8 through 22 )
3X-RAY DIFFRACTION3chain 'A' and (resid 23 through 34 )
4X-RAY DIFFRACTION4chain 'A' and (resid 35 through 44 )
5X-RAY DIFFRACTION5chain 'A' and (resid 45 through 49 )
6X-RAY DIFFRACTION6chain 'A' and (resid 50 through 59 )
7X-RAY DIFFRACTION7chain 'A' and (resid 60 through 65 )
8X-RAY DIFFRACTION8chain 'A' and (resid 66 through 74 )
9X-RAY DIFFRACTION9chain 'C' and (resid 1 through 22 )
10X-RAY DIFFRACTION10chain 'C' and (resid 23 through 34 )
11X-RAY DIFFRACTION11chain 'C' and (resid 35 through 44 )
12X-RAY DIFFRACTION12chain 'C' and (resid 45 through 54 )
13X-RAY DIFFRACTION13chain 'C' and (resid 55 through 74 )
14X-RAY DIFFRACTION14chain 'E' and (resid 1 through 7 )
15X-RAY DIFFRACTION15chain 'E' and (resid 8 through 16 )
16X-RAY DIFFRACTION16chain 'E' and (resid 17 through 22 )
17X-RAY DIFFRACTION17chain 'E' and (resid 23 through 34 )
18X-RAY DIFFRACTION18chain 'E' and (resid 35 through 44 )
19X-RAY DIFFRACTION19chain 'E' and (resid 45 through 49 )
20X-RAY DIFFRACTION20chain 'E' and (resid 50 through 54 )
21X-RAY DIFFRACTION21chain 'E' and (resid 55 through 59 )
22X-RAY DIFFRACTION22chain 'E' and (resid 60 through 65 )
23X-RAY DIFFRACTION23chain 'E' and (resid 66 through 75 )
24X-RAY DIFFRACTION24chain 'G' and (resid 1 through 22 )
25X-RAY DIFFRACTION25chain 'G' and (resid 23 through 34 )
26X-RAY DIFFRACTION26chain 'G' and (resid 35 through 49 )
27X-RAY DIFFRACTION27chain 'G' and (resid 50 through 54 )
28X-RAY DIFFRACTION28chain 'G' and (resid 55 through 65 )
29X-RAY DIFFRACTION29chain 'G' and (resid 66 through 74 )
30X-RAY DIFFRACTION30chain 'I' and (resid 1 through 22 )
31X-RAY DIFFRACTION31chain 'I' and (resid 23 through 34 )
32X-RAY DIFFRACTION32chain 'I' and (resid 35 through 49 )
33X-RAY DIFFRACTION33chain 'I' and (resid 50 through 54 )
34X-RAY DIFFRACTION34chain 'I' and (resid 55 through 65 )
35X-RAY DIFFRACTION35chain 'I' and (resid 66 through 73 )
36X-RAY DIFFRACTION36chain 'K' and (resid 1 through 6 )
37X-RAY DIFFRACTION37chain 'K' and (resid 7 through 22 )
38X-RAY DIFFRACTION38chain 'K' and (resid 23 through 34 )
39X-RAY DIFFRACTION39chain 'K' and (resid 35 through 49 )
40X-RAY DIFFRACTION40chain 'K' and (resid 50 through 54 )
41X-RAY DIFFRACTION41chain 'K' and (resid 55 through 73 )
42X-RAY DIFFRACTION42chain 'D' and (resid 33 through 74 )
43X-RAY DIFFRACTION43chain 'D' and (resid 75 through 90 )
44X-RAY DIFFRACTION44chain 'D' and (resid 91 through 105 )
45X-RAY DIFFRACTION45chain 'D' and (resid 106 through 121 )
46X-RAY DIFFRACTION46chain 'H' and (resid 33 through 52 )
47X-RAY DIFFRACTION47chain 'H' and (resid 53 through 62 )
48X-RAY DIFFRACTION48chain 'H' and (resid 63 through 73 )
49X-RAY DIFFRACTION49chain 'H' and (resid 74 through 86 )
50X-RAY DIFFRACTION50chain 'H' and (resid 87 through 103 )
51X-RAY DIFFRACTION51chain 'H' and (resid 104 through 121 )
52X-RAY DIFFRACTION52chain 'L' and (resid 32 through 52 )
53X-RAY DIFFRACTION53chain 'L' and (resid 53 through 62 )
54X-RAY DIFFRACTION54chain 'L' and (resid 63 through 73 )
55X-RAY DIFFRACTION55chain 'L' and (resid 74 through 103 )
56X-RAY DIFFRACTION56chain 'L' and (resid 104 through 120 )
57X-RAY DIFFRACTION57chain 'B' and (resid 32 through 52 )
58X-RAY DIFFRACTION58chain 'B' and (resid 53 through 73 )
59X-RAY DIFFRACTION59chain 'B' and (resid 74 through 104 )
60X-RAY DIFFRACTION60chain 'B' and (resid 105 through 120 )
61X-RAY DIFFRACTION61chain 'F' and (resid 32 through 73 )
62X-RAY DIFFRACTION62chain 'F' and (resid 74 through 122 )
63X-RAY DIFFRACTION63chain 'J' and (resid 32 through 73 )
64X-RAY DIFFRACTION64chain 'J' and (resid 74 through 105 )
65X-RAY DIFFRACTION65chain 'J' and (resid 106 through 121 )

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