根拠: SAXS, Rg from SAXS: 22A, Rg from structure coordinates for dimer: 20A, gel filtration, - column - Superdex 200 - buffer 20mM Tris, 150mM NaCl, pH 8,0 - molecular mass in solution (by gel ...根拠: SAXS, Rg from SAXS: 22A, Rg from structure coordinates for dimer: 20A, gel filtration, - column - Superdex 200 - buffer 20mM Tris, 150mM NaCl, pH 8,0 - molecular mass in solution (by gel filtration) - 40000 Da (calibrated by ovalbumin - 43 kDa, bovie serum albumin - 66 kDa, aldolase - 158 kDa) - molecular mass according to aminoacid sequence - 18341 Da, equilibrium centrifugation, Analytical ultracentrifugation: buffer 20mM Tris, 150mM NaCl, pH 8,0 sw=3,090 and sw(20,w)=3.207 molecular mass in solution (by AUC) - 38999 Da molecular mass according to amino acid sequence - 18341 Da
マシュー密度: 2.27 Å3/Da / 溶媒含有率: 46 % 解説: Crystal shape - red wedge block 300x80x80 um; the crystal was soaked for 15 min in a maternal drop containing additionally 10 mM sodium dithionite.
結晶化
温度: 298 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 6.7 詳細: 20.7% (w/v) PEG3350, 200 mM MgCl2, 0.01 M KCN, 7.5% (v/v) glycerol, 0.1 M MMT buffer system (DL-Malic acid, MES monohydrate, Tris, NaOH, HCl), pH 6.7
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データ収集
回折
平均測定温度: 100 K
放射光源
由来: シンクロトロン / サイト: PETRA III, EMBL c/o DESY / ビームライン: P13 (MX1) / 波長: 1 Å
解像度: 1.8→49.17 Å / Cor.coef. Fo:Fc: 0.958 / SU B: 2.748 / SU ML: 0.082 / 交差検証法: THROUGHOUT / ESU R: 0.138 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS