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- PDB-5nz1: Structure of Transcriptional Regulatory Repressor Protein - EthR ... -

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Basic information

Entry
Database: PDB / ID: 5nz1
TitleStructure of Transcriptional Regulatory Repressor Protein - EthR from Mycobacterium Tuberculosis in complex with sutezolid
ComponentsHTH-type transcriptional regulator EthR
KeywordsTRANSCRIPTION / EthR / represor / boosting effect
Function / homology
Function and homology information


transcription cis-regulatory region binding / DNA-binding transcription factor activity / response to antibiotic / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / cytosol
Similarity search - Function
: / Transcriptional regulator EthR, C-terminal domain / : / Tetracycline Repressor, domain 2 / Tetracyclin repressor-like, C-terminal domain superfamily / Tetracycline Repressor; domain 2 / Bacterial regulatory proteins, tetR family / DNA-binding HTH domain, TetR-type / TetR-type HTH domain profile. / Homeodomain-like ...: / Transcriptional regulator EthR, C-terminal domain / : / Tetracycline Repressor, domain 2 / Tetracyclin repressor-like, C-terminal domain superfamily / Tetracycline Repressor; domain 2 / Bacterial regulatory proteins, tetR family / DNA-binding HTH domain, TetR-type / TetR-type HTH domain profile. / Homeodomain-like / Homeobox-like domain superfamily / Arc Repressor Mutant, subunit A / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Chem-9EN / HTH-type transcriptional regulator EthR
Similarity search - Component
Biological speciesMycobacterium tuberculosis (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.33 Å
AuthorsMendes, V. / Blaszczyk, M. / Nikiforov, P.O. / Blundell, T.L.
Funding support United States, 1items
OrganizationGrant numberCountry
Bill & Melinda Gates Foundation United States
CitationJournal: To Be Published
Title: The antibiotics linezolid and sutezolid are ligands for Mycobacterium tuberculosis EthR
Authors: Nikiforov, P.O. / Blaszczyk, M. / Mendes, V. / Boshoff, H.I. / Barry, C.E. / Blundell, T.L. / Abell, C.
History
DepositionMay 12, 2017Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 30, 2018Provider: repository / Type: Initial release
Revision 1.1Jan 17, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HTH-type transcriptional regulator EthR
B: HTH-type transcriptional regulator EthR
C: HTH-type transcriptional regulator EthR
D: HTH-type transcriptional regulator EthR
E: HTH-type transcriptional regulator EthR
F: HTH-type transcriptional regulator EthR
G: HTH-type transcriptional regulator EthR
H: HTH-type transcriptional regulator EthR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)203,13411
Polymers202,0748
Non-polymers1,0603
Water4,053225
1
A: HTH-type transcriptional regulator EthR
B: HTH-type transcriptional regulator EthR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)50,8723
Polymers50,5192
Non-polymers3531
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2800 Å2
ΔGint-23 kcal/mol
Surface area17390 Å2
MethodPISA
2
C: HTH-type transcriptional regulator EthR
D: HTH-type transcriptional regulator EthR
hetero molecules


Theoretical massNumber of molelcules
Total (without water)51,2254
Polymers50,5192
Non-polymers7072
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2740 Å2
ΔGint-21 kcal/mol
Surface area16920 Å2
MethodPISA
3
E: HTH-type transcriptional regulator EthR
F: HTH-type transcriptional regulator EthR


Theoretical massNumber of molelcules
Total (without water)50,5192
Polymers50,5192
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2700 Å2
ΔGint-20 kcal/mol
Surface area17730 Å2
MethodPISA
4
G: HTH-type transcriptional regulator EthR
H: HTH-type transcriptional regulator EthR


Theoretical massNumber of molelcules
Total (without water)50,5192
Polymers50,5192
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2600 Å2
ΔGint-25 kcal/mol
Surface area17690 Å2
MethodPISA
Unit cell
Length a, b, c (Å)85.858, 144.841, 150.741
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein
HTH-type transcriptional regulator EthR


Mass: 25259.254 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Strain: ATCC 25618 / H37Rv / Gene: ethR, etaR, Rv3855 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P9WMC1
#2: Chemical ChemComp-9EN / ~{N}-[[(5~{S})-3-(3-fluoranyl-4-thiomorpholin-4-yl-phenyl)-2-oxidanylidene-1,3-oxazolidin-5-yl]methyl]ethanamide


Mass: 353.412 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C16H20FN3O3S
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 225 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.32 Å3/Da / Density % sol: 46.96 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.1 HEPES 25% PEG 550 MME

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.98 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 17, 2016
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.98 Å / Relative weight: 1
ReflectionResolution: 2.33→150.74 Å / Num. obs: 80962 / % possible obs: 99.9 % / Redundancy: 6.6 % / Biso Wilson estimate: 52.05 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.065 / Rpim(I) all: 0.028 / Rrim(I) all: 0.071 / Net I/σ(I): 15.9
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique allCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
2.33-2.466.80.72179408117110.8030.2980.7812.4100
7.37-150.7460.0381666327890.9970.0170.04240.299.5

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Processing

Software
NameVersionClassification
PHENIX1.10_2155refinement
Aimless0.5.15data scaling
PDB_EXTRACT3.22data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1T56

1t56


Resolution: 2.33→104.442 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 25.38
RfactorNum. reflection% reflection
Rfree0.2437 4140 5.12 %
Rwork0.1913 --
obs0.194 80875 99.88 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 188.31 Å2 / Biso mean: 75.0522 Å2 / Biso min: 30.24 Å2
Refinement stepCycle: final / Resolution: 2.33→104.442 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11576 0 72 225 11873
Biso mean--104.25 58.01 -
Num. residues----1522
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00911925
X-RAY DIFFRACTIONf_angle_d0.94216285
X-RAY DIFFRACTIONf_chiral_restr0.0491899
X-RAY DIFFRACTIONf_plane_restr0.0062119
X-RAY DIFFRACTIONf_dihedral_angle_d17.0917079
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.33-2.35650.36411230.280325342657100
2.3565-2.38420.3221170.260825672684100
2.3842-2.41330.26561310.245325142645100
2.4133-2.44380.3111150.242725622677100
2.4438-2.4760.32461520.240225172669100
2.476-2.50990.29441360.223625412677100
2.5099-2.54580.29291230.225425292652100
2.5458-2.58380.26741490.212825172666100
2.5838-2.62420.27721600.230125212681100
2.6242-2.66720.29571380.221825202658100
2.6672-2.71320.2681240.216825582682100
2.7132-2.76250.26661400.208525392679100
2.7625-2.81570.27171370.20825212658100
2.8157-2.87310.29131610.209825122673100
2.8731-2.93560.25771580.206825502708100
2.9356-3.00390.30081170.221825502667100
3.0039-3.0790.27971270.236325802707100
3.079-3.16230.30431140.230425512665100
3.1623-3.25530.28991460.225325172663100
3.2553-3.36040.29411430.209525712714100
3.3604-3.48050.28671510.201325492700100
3.4805-3.61990.22141180.192725772695100
3.6199-3.78460.22751510.183825272678100
3.7846-3.98420.241710.174525442715100
3.9842-4.23380.20631380.163425862724100
4.2338-4.56070.20411480.153225752723100
4.5607-5.01970.21251330.155326002733100
5.0197-5.7460.24681270.190526242751100
5.746-7.23910.2631360.2012647278399
7.2391-104.54660.18811560.16612735289199
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.8485-3.78364.86468.7284-4.79087.4025-0.0564-0.3155-0.16390.10130.23850.1460.4441-0.2029-0.23820.44430.00560.07310.5339-0.04830.3591-18.8522-61.370710.3297
28.64983.21012.10747.134-1.7099.58840.3192-1.0550.12470.7816-0.102-0.4248-0.3066-0.9991-0.21530.64770.0206-0.04420.4957-0.04990.3449-17.3173-60.573720.153
35.6012-2.1775-5.81693.90052.64327.6216-0.0415-0.0724-0.15210.0252-0.09290.0183-0.18190.57260.19090.42850.0384-0.05810.5178-0.00580.3842-6.1721-58.32631.7146
45.45032.2001-2.849.1755-3.67717.9939-0.4870.717-0.9439-1.1862-0.1034-0.57151.0160.11350.48150.58260.05450.05560.4613-0.14260.582-5.1686-60.3794-15.4649
58.39651.50350.18375.59514.69174.04880.11630.02760.7818-0.11610.08580.6321-0.5388-0.5202-0.21490.52380.01550.06050.52490.09790.4073-15.8565-51.56151.2552
66.0683-1.4643-5.07783.35661.24253.6615-0.3651-0.56190.08790.16860.1651-0.18190.32581.26030.07980.2958-0.0356-0.03860.4057-0.07110.40820.3816-49.3978-7.7977
78.85350.7652-1.91193.14830.06733.33940.02420.2602-0.1578-0.1091-0.07660.02580.14180.05390.04730.40210.0186-0.02390.3123-0.03310.2866-8.5595-49.6328-18.1209
82.9922-0.5411-3.17349.36451.40233.63450.22010.18970.3551-0.52020.5271-0.0525-0.6439-0.1879-0.74270.4647-0.00220.0280.502-0.02940.5654-23.0413-18.66510.2933
97.1026-4.2759-3.69257.81765.6346.45190.1169-2.29730.71850.20.66060.0458-0.48781.1109-0.60270.5003-0.03380.04910.7457-0.2040.5491-22.531-21.86079.1785
106.8458-3.8632-1.94829.28441.56938.2267-0.57820.788-0.26790.6782-0.031.0977-0.1645-0.82750.28190.4057-0.00350.09570.6067-0.04760.5354-33.545-20.56446.1986
116.1619-1.5714-5.65413.13652.27715.54690.4060.56320.1552-0.6456-0.23840.2882-0.6685-1.4658-0.19650.42010.0119-0.07870.3155-0.00830.577-25.4889-27.9122-12.4512
125.2886-5.8094-2.49436.42512.98642.377-0.5180.1948-1.29290.07960.75642.0824-1.4494-0.6853-0.18121.31590.3448-0.02651.8839-0.1071.471-16.3228-30.212-35.3045
133.7897-0.51980.30568.90446.23844.69470.11180.30230.5207-1.38460.2201-0.3321-1.05930.2184-0.29590.6646-0.0931-0.01120.43040.11470.5827-13.4002-32.0227-22.8659
145.32345.506-7.31825.5172-6.83189.761-0.0164-0.5051-0.10440.2872-0.3954-0.270.12970.19850.27770.45830.03620.03540.3244-0.09160.4788-19.1327-33.4149-3.4916
155.6173-3.6157-4.59674.75534.30284.19730.34210.44270.0228-0.453-0.10160.32030.0154-0.4287-0.18410.4462-0.034-0.04270.2978-0.05780.4851-24.6471-40.2857-19.956
165.75230.67573.00625.36350.04522.2834-0.0736-0.00050.0656-0.4090.00640.2437-0.4924-0.09510.07660.3696-0.0146-0.00090.23550.0190.3193-15.3273-43.7457-20.5705
178.053-0.30321.14835.8236-3.22823.19630.13520.1390.0298-0.0521-0.0373-0.6475-0.26340.4661-0.06510.36110.01680.01580.2918-0.05730.3568-6.3268-39.6467-19.882
183.3311-4.23970.46349.0695-4.21638.0942-0.1313-0.8216-0.22310.8935-0.02580.263-0.3602-0.27790.09550.4245-0.0062-0.01860.55930.03350.49233.3352-13.4492.3673
193.97-5.24771.95196.7082-1.92848.8734-0.152-0.46540.05860.46110.2992-0.56210.06440.3593-0.13880.32390.0062-0.11050.50140.05090.525210.6521-19.98412.697
204.13524.39313.56425.34332.15234.6379-0.02650.03060.4170.0248-0.12540.55790.0105-0.36690.13020.31490.0207-0.00530.36450.03920.3773-7.3562-16.4844-7.8578
215.22891.38095.40465.34830.33085.51580.0392-0.41660.99990.0656-0.3140.83610.51-0.52040.19940.44860.07580.0420.6204-0.02090.5856-22.2071-8.6606-9.5632
225.0096-0.2244.38082.1158-1.44635.35310.02880.1592-0.02-0.21470.0480.14160.0563-0.3101-0.08750.36260.0205-0.04460.356-0.05350.4273-13.1297-10.3377-18.7518
234.17350.95282.64743.36561.71657.3632-0.2653-0.04680.4054-0.16790.08860.5561-0.8224-0.39420.22630.49630.0696-0.06340.38170.08990.578-17.8560.1638-15.3146
244.98322.0292-0.56863.6937-4.52056.3353-0.17650.54230.0212-0.3345-0.0699-0.729-0.70930.80980.20960.5925-0.1766-0.01220.63510.06180.409616.445312.8137-28.7375
253.01830.98183.29553.16-0.57794.83890.47990.78022.0011-0.7364-0.60280.5796-0.3949-0.35760.13130.9236-0.0445-0.18780.57270.07120.8674-5.590618.5966-27.0718
264.9734-1.9253-3.23488.08230.80714.33850.19391.50662.1242-0.7431-0.36540.0902-1.4208-0.9412-0.10920.8036-0.013-0.22660.68870.20080.6957-14.653412.0494-30.6942
274.7986-0.41492.72322.4321-1.59068.0096-0.0879-0.15680.1268-0.3005-0.0438-0.0740.7599-0.29460.00380.5914-0.07130.02020.39250.02780.52.62356.0844-22.3632
284.1075-0.2258-0.57273.31720.41123.0301-0.1638-0.02060.6248-0.1572-0.02570.6291-0.9117-0.23090.15990.68910.1022-0.1520.34420.04140.6372-15.48128.7326-21.0227
296.776-0.9768-5.20644.92960.83898.2566-0.03071.3823-0.2946-0.5660.0130.56330.2608-0.93490.02250.394-0.0646-0.08040.6602-0.02390.48559.4426-10.9743-34.3285
304.392-1.8752-5.83113.16364.19687.7444-0.8662-0.5243-1.37170.54010.53610.03371.07810.62830.38720.5876-0.0405-0.00290.5390.08160.758629.7444-21.5583-35.1388
318.6511-1.4194-2.64933.00841.7452.0364-0.00790.0357-0.4659-0.05760.0023-0.08740.2611-0.2469-0.02360.6035-0.0167-0.01050.49820.09770.312936.0592-17.2706-39.1324
325.79350.41370.47965.31861.32667.3436-0.15620.55290.1564-0.29640.0068-0.18220.413-0.39830.17240.4195-0.03320.04890.43470.08760.394642.742-16.1627-47.3931
334.8182-0.8055-0.16392.9108-0.0588.1081-0.3326-0.07980.47040.367-0.0367-0.8338-0.28950.42530.3670.4884-0.002-0.13040.58660.07840.611837.495922.4209-43.1673
346.6533-3.42590.41654.59870.07761.47360.73950.6164-0.6216-1.4675-0.7539-0.0179-0.07770.3682-0.01230.64590.0647-0.01010.70770.18390.617742.592913.1666-54.66
358.1863-1.2367-1.04146.70120.93893.6172-0.36240.20690.1446-0.10290.2466-0.82180.07010.1920.12810.42740.03890.00670.55260.2380.643848.26631.7215-49.0647
363.0953-1.1274-0.28184.57850.25731.3716-0.13070.44540.1174-0.3365-0.1497-0.2077-0.03040.01390.2480.43130.01360.02370.50230.09720.448545.4082-6.8298-52.931
379.3286-2.07121.01152.6867-1.16561.15960.0777-0.1951-0.31291.57660.2799-1.88470.21340.9446-0.23710.93620.0149-0.63090.9702-0.24181.510723.3263-52.3595-82.983
383.465-1.14361.1182.6156-4.80457.91780.3020.9615-0.7343-0.32520.7559-1.3113-0.44251.0335-0.62440.8797-0.2285-0.02340.9837-0.43551.265122.3744-35.9335-90.7893
394.99092.6301-1.5692.9215-3.78497.387-0.7264-2.30960.8649-0.84840.75981.24820.69850.0364-0.22721.3026-0.32580.07811.88660.01090.931810.9527-22.2067-103.2235
401.61160.07040.65765.4466-1.20623.05550.2262-0.0861-0.07180.65350.26520.5766-0.0783-0.0583-0.51320.608-0.21870.09960.827-0.11170.677215.0459-23.4466-84.2164
416.45063.6075-1.73674.1565-2.53395.63022.1939-0.47840.87931.4655-0.73860.614-1.7851-0.4299-1.48121.8802-0.15890.1510.7396-0.0191.11518.9285-28.4707-48.4639
424.7474.3404-0.77284.4382-1.72662.55640.4645-0.5146-1.9061.2673-0.9854-0.20893.0954-0.14890.42092.78330.04390.17770.86040.11281.36316.3683-37.0053-43.7537
430.9685-0.33521.41881.79120.21851.53710.0904-0.3940.15571.84490.25930.8578-0.3328-1.2942-0.28941.9576-0.02850.74461.1822-0.04461.11086.7645-17.0414-60.5992
447.53061.55113.39121.60681.3922.83731.0252-0.0725-0.20851.3546-1.04150.9587-0.42580.1890.40671.3697-0.22530.31020.8215-0.02640.901815.6148-28.8735-60.9316
451.54260.93110.69412.3491-0.49820.15320.2624-0.37040.02721.49580.74532.9296-0.2859-0.9548-0.46331.2840.10840.84191.3358-0.03311.7689-2.0872-18.8652-72.929
463.79151.98883.88016.69972.02494.27060.71010.44510.27041.8475-0.21231.26511.53880.0162-0.49851.1706-0.04980.47230.9246-0.01941.00479.5407-20.9043-73.2918
477.1153-1.94671.91657.5658-0.22929.55630.7583-0.90620.24481.7074-0.2781.6079-0.2577-0.0183-0.41211.025-0.21720.4110.7894-0.26230.851113.1987-12.4282-76.0462
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 22 through 46 )A22 - 46
2X-RAY DIFFRACTION2chain 'A' and (resid 47 through 67 )A47 - 67
3X-RAY DIFFRACTION3chain 'A' and (resid 68 through 91 )A68 - 91
4X-RAY DIFFRACTION4chain 'A' and (resid 92 through 116 )A92 - 116
5X-RAY DIFFRACTION5chain 'A' and (resid 117 through 131 )A117 - 131
6X-RAY DIFFRACTION6chain 'A' and (resid 132 through 158 )A132 - 158
7X-RAY DIFFRACTION7chain 'A' and (resid 159 through 215 )A159 - 215
8X-RAY DIFFRACTION8chain 'B' and (resid 23 through 38 )B23 - 38
9X-RAY DIFFRACTION9chain 'B' and (resid 39 through 58 )B39 - 58
10X-RAY DIFFRACTION10chain 'B' and (resid 59 through 67 )B59 - 67
11X-RAY DIFFRACTION11chain 'B' and (resid 68 through 92 )B68 - 92
12X-RAY DIFFRACTION12chain 'B' and (resid 93 through 98 )B93 - 98
13X-RAY DIFFRACTION13chain 'B' and (resid 99 through 115 )B99 - 115
14X-RAY DIFFRACTION14chain 'B' and (resid 116 through 131 )B116 - 131
15X-RAY DIFFRACTION15chain 'B' and (resid 132 through 158 )B132 - 158
16X-RAY DIFFRACTION16chain 'B' and (resid 159 through 188 )B159 - 188
17X-RAY DIFFRACTION17chain 'B' and (resid 189 through 215 )B189 - 215
18X-RAY DIFFRACTION18chain 'C' and (resid 22 through 46 )C22 - 46
19X-RAY DIFFRACTION19chain 'C' and (resid 47 through 67 )C47 - 67
20X-RAY DIFFRACTION20chain 'C' and (resid 68 through 90 )C68 - 90
21X-RAY DIFFRACTION21chain 'C' and (resid 91 through 116 )C91 - 116
22X-RAY DIFFRACTION22chain 'C' and (resid 117 through 167 )C117 - 167
23X-RAY DIFFRACTION23chain 'C' and (resid 168 through 215 )C168 - 215
24X-RAY DIFFRACTION24chain 'D' and (resid 22 through 67 )D22 - 67
25X-RAY DIFFRACTION25chain 'D' and (resid 68 through 98 )D68 - 98
26X-RAY DIFFRACTION26chain 'D' and (resid 99 through 116 )D99 - 116
27X-RAY DIFFRACTION27chain 'D' and (resid 117 through 131 )D117 - 131
28X-RAY DIFFRACTION28chain 'D' and (resid 132 through 214 )D132 - 214
29X-RAY DIFFRACTION29chain 'E' and (resid 22 through 67 )E22 - 67
30X-RAY DIFFRACTION30chain 'E' and (resid 68 through 98 )E68 - 98
31X-RAY DIFFRACTION31chain 'E' and (resid 99 through 159 )E99 - 159
32X-RAY DIFFRACTION32chain 'E' and (resid 160 through 214 )E160 - 214
33X-RAY DIFFRACTION33chain 'F' and (resid 22 through 57 )F22 - 57
34X-RAY DIFFRACTION34chain 'F' and (resid 58 through 98 )F58 - 98
35X-RAY DIFFRACTION35chain 'F' and (resid 99 through 128 )F99 - 128
36X-RAY DIFFRACTION36chain 'F' and (resid 129 through 214 )F129 - 214
37X-RAY DIFFRACTION37chain 'G' and (resid 23 through 67 )G23 - 67
38X-RAY DIFFRACTION38chain 'G' and (resid 68 through 92 )G68 - 92
39X-RAY DIFFRACTION39chain 'G' and (resid 93 through 101 )G93 - 101
40X-RAY DIFFRACTION40chain 'G' and (resid 102 through 214 )G102 - 214
41X-RAY DIFFRACTION41chain 'H' and (resid 23 through 44 )H23 - 44
42X-RAY DIFFRACTION42chain 'H' and (resid 45 through 67 )H45 - 67
43X-RAY DIFFRACTION43chain 'H' and (resid 68 through 116 )H68 - 116
44X-RAY DIFFRACTION44chain 'H' and (resid 117 through 131 )H117 - 131
45X-RAY DIFFRACTION45chain 'H' and (resid 132 through 167 )H132 - 167
46X-RAY DIFFRACTION46chain 'H' and (resid 168 through 188 )H168 - 188
47X-RAY DIFFRACTION47chain 'H' and (resid 189 through 215 )H189 - 215

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