Mass: 14066.904 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The sequence includes N-terminal Mellitin signal peptide, cloning artefect (ADP) and C-terminal His-tag. Source: (gene. exp.) Homo sapiens (human) / Gene: TIE1, TIE Production host: Spodoptera aff. frugiperda 2 RZ-2014 (butterflies/moths) References: UniProt: P35590, receptor protein-tyrosine kinase
Mass: 18.015 Da / Num. of mol.: 73 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2 Å3/Da / Density % sol: 37 % / Description: Hexagonal rods
Crystal grow
Temperature: 294 K / Method: vapor diffusion, sitting drop Details: 0.1 M Tris buffer at pH 8.0 - 9.0 and 18-24% PEG 8000 (w/v) PH range: 8.0 - 9.0 / Temp details: Room temperature
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Data collection
Diffraction
Mean temperature: 100 K / Ambient temp details: Liguid nitrogen cooling system