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Yorodumi- PDB-5maw: Crystal structure of the flagellin-FliS complex from Bacillus subtilis -
+Open data
-Basic information
Entry | Database: PDB / ID: 5maw | ||||||
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Title | Crystal structure of the flagellin-FliS complex from Bacillus subtilis | ||||||
Components |
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Keywords | CHAPERONE / flagellum / type-3-secretion | ||||||
Function / homology | Function and homology information bacterial-type flagellum / bacterial-type flagellum assembly / bacterial-type flagellum-dependent cell motility / structural molecule activity / extracellular region / cytosol Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Altegoer, F. / Bange, G. | ||||||
Citation | Journal: Sci Rep / Year: 2018 Title: FliS/flagellin/FliW heterotrimer couples type III secretion and flagellin homeostasis. Authors: Altegoer, F. / Mukherjee, S. / Steinchen, W. / Bedrunka, P. / Linne, U. / Kearns, D.B. / Bange, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5maw.cif.gz | 94.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5maw.ent.gz | 69.8 KB | Display | PDB format |
PDBx/mmJSON format | 5maw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ma/5maw ftp://data.pdbj.org/pub/pdb/validation_reports/ma/5maw | HTTPS FTP |
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-Related structure data
Related structure data | 6gowC 1io1S 1vh6S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 33545.180 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: hag, BSU35360 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P02968 |
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#2: Protein | Mass: 15146.071 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: fliS, BSU35330 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P39739 |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.22 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.2 M Lithium sulfate, 0.1 M Tris pH 7.0, 2.0 M Ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.9723 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 13, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9723 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→48.57 Å / Num. obs: 61470 / % possible obs: 99 % / Redundancy: 4.1 % / CC1/2: 0.998 / Rmerge(I) obs: 0.04399 / Net I/σ(I): 18.17 |
Reflection shell | Resolution: 1.5→1.554 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 5.4 / Num. unique obs: 6152 / CC1/2: 0.97 / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1IO1, 1VH6 Resolution: 1.5→48.568 Å / SU ML: 0.14 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 21.58 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→48.568 Å
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Refine LS restraints |
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LS refinement shell |
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