Mass: 18.015 Da / Num. of mol.: 104 / Source method: isolated from a natural source / Formula: H2O
Sequence details
At the time of annotation, Uniprot Id not available for this entry. Should map to Uniprot entry ...At the time of annotation, Uniprot Id not available for this entry. Should map to Uniprot entry C0HK15 with mutation at K15-A16.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.62 Å3/Da / Density % sol: 53 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: Protein: 7 mg/mL in 50mM sodium Acetate pH 5.5 Precipitant: 36% MPEG 2K, 0.45 M NaCl, 0.9 M KSCN, 0.1 M imidazole HCl, pH 7.5. Cryoprotectant: 5 % diethylene glycol, 5 % ethylene glycol, 5 % ...Details: Protein: 7 mg/mL in 50mM sodium Acetate pH 5.5 Precipitant: 36% MPEG 2K, 0.45 M NaCl, 0.9 M KSCN, 0.1 M imidazole HCl, pH 7.5. Cryoprotectant: 5 % diethylene glycol, 5 % ethylene glycol, 5 % MPD, 5 % glycerol, 5 % 1,2-propanediol, 5 % DMSO, 18% MPEG-2K, 100 mM linear mixed buffer 1: sodium acetate, ADA, and bicine, 20% at pH 4 and 80% at pH 10. Temp details: cooled incubator
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Data collection
Diffraction
Mean temperature: 100 K / Ambient temp details: cryostream