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- PDB-5m1x: Crystal structure of S. cerevisiae Rfa1 N-OB domain mutant (K45E) -
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Open data
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Basic information
Entry | Database: PDB / ID: 5m1x | ||||||
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Title | Crystal structure of S. cerevisiae Rfa1 N-OB domain mutant (K45E) | ||||||
![]() | Replication factor A protein 1 | ||||||
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Function / homology | ![]() heteroduplex formation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Seeber, A. / Hegnauer, A.M. / Hustedt, N. / Deshpande, I. / Poli, J. / Eglinger, J. / Pasero, P. / Gut, H. / Shinohara, M. / Hopfner, K.P. ...Seeber, A. / Hegnauer, A.M. / Hustedt, N. / Deshpande, I. / Poli, J. / Eglinger, J. / Pasero, P. / Gut, H. / Shinohara, M. / Hopfner, K.P. / Shimada, K. / Gasser, S.M. | ||||||
![]() | ![]() Title: RPA Mediates Recruitment of MRX to Forks and Double-Strand Breaks to Hold Sister Chromatids Together. Authors: Seeber, A. / Hegnauer, A.M. / Hustedt, N. / Deshpande, I. / Poli, J. / Eglinger, J. / Pasero, P. / Gut, H. / Shinohara, M. / Hopfner, K.P. / Shimada, K. / Gasser, S.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 226.8 KB | Display | ![]() |
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PDB format | ![]() | 192 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15616.754 Da / Num. of mol.: 4 / Mutation: K45E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.11 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 200 mM ammonium fluoride 20 % (w/v) PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Jun 5, 2014 / Details: Mirrors |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→44.26 Å / Num. obs: 80762 / % possible obs: 95.4 % / Observed criterion σ(I): -3 / Redundancy: 2.4 % / Biso Wilson estimate: 23.08 Å2 / CC1/2: 0.99 / Rsym value: 0.079 / Net I/σ(I): 8.02 |
Reflection shell | Resolution: 1.8→1.85 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.53 / Mean I/σ(I) obs: 1.74 / CC1/2: 0.68 / % possible all: 86.2 |
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Processing
Software |
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Refinement | Method to determine structure![]() ![]() Details: Experimental details report unique reflections for unmerged Friedel pairs. Refinement was carried out on the same data with merged Friedel pairs. Automatic form factor correction at 0.97941 ...Details: Experimental details report unique reflections for unmerged Friedel pairs. Refinement was carried out on the same data with merged Friedel pairs. Automatic form factor correction at 0.97941 A was used for Se atoms in Buster.
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Displacement parameters | Biso mean: 31.72 Å2
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Refine analyze | Luzzati coordinate error obs: 0.209 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 1.8→44.26 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.85 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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