温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: The HODM-heme subunit was crystallised at 3 mg ml-1 in 50 mM KPi, 100 mM KCl, pH 7.5. Initial crystallisation conditions were identified using the JCSG+ matrix screen (Molecular dimensions). ...詳細: The HODM-heme subunit was crystallised at 3 mg ml-1 in 50 mM KPi, 100 mM KCl, pH 7.5. Initial crystallisation conditions were identified using the JCSG+ matrix screen (Molecular dimensions). Crystals suitable for diffraction experiments were obtained by sitting drop vapour diffusion at 277 K in 400 nL drops containing equal volumes of protein and a solution containing 30% PEG 2K MME and 0.1 M potassium thiocyanate.
解像度: 1.76→33.01 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.952 / SU B: 1.622 / SU ML: 0.053 / 交差検証法: THROUGHOUT / ESU R: 0.09 / ESU R Free: 0.088 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS