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- PDB-5lhu: ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5lhu | ||||||
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Title | ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L-Histidine | ||||||
![]() | ATP phosphoribosyltransferase | ||||||
![]() | TRANSFERASE / ATP-PRTase / ACT / His G / Histidine biosynthesis | ||||||
Function / homology | ![]() ATP phosphoribosyltransferase / ATP phosphoribosyltransferase activity / L-histidine biosynthetic process / AMP binding / magnesium ion binding / ATP binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | de Chiara, C. / Pisco, J.P. / de Carvalho, L.P. / Smerdon, S.J. / Walker, P.A. / Ogrodowicz, R. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Uncoupling conformational states from activity in an allosteric enzyme. Authors: Pisco, J.P. / Chiara, C. / Pacholarz, K.J. / Garza-Garcia, A. / Ogrodowicz, R.W. / Walker, P.A. / Barran, P.E. / Smerdon, S.J. / Carvalho, L.P.S. #1: Journal: Biochemistry / Year: 2012 Title: Mechanism of feedback allosteric inhibition of ATP phosphoribosyltransferase. Authors: Pedreno, S. / Pisco, J.P. / Larrouy-Maumus, G. / Kelly, G. / de Carvalho, L.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.8 KB | Display | ![]() |
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PDB format | ![]() | 52.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 464.3 KB | Display | ![]() |
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Full document | ![]() | 465.1 KB | Display | |
Data in XML | ![]() | 13.1 KB | Display | |
Data in CIF | ![]() | 17.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5lhtC ![]() 1nh8S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 31686.023 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 25618 / H37Rv / Gene: hisG, Rv2121c, MTCY261.17c / Plasmid: pJ411 / Production host: ![]() ![]() | ||||
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#2: Chemical | ChemComp-HIS / | ||||
#3: Chemical | ChemComp-SO4 / #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.12 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, sitting drop / Details: 2.5 %(v/v) Isopropanol, 1.0 M Ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 6, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9282 Å / Relative weight: 1 |
Reflection | Resolution: 2.02→47.08 Å / Num. obs: 22068 / % possible obs: 99.7 % / Redundancy: 4.8 % / Net I/σ(I): 22.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1NH8 Resolution: 2.02→42.444 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.64 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.02→42.444 Å
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Refine LS restraints |
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LS refinement shell |
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