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Yorodumi- PDB-5lfu: Myelin-associated glycoprotein (MAG) glycosylated and lysine-meth... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5lfu | |||||||||
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Title | Myelin-associated glycoprotein (MAG) glycosylated and lysine-methylated full extracellular domain | |||||||||
Components | Myelin-associated glycoprotein | |||||||||
Keywords | CELL ADHESION / Myelin / Signaling | |||||||||
Function / homology | Function and homology information mesaxon / Axonal growth inhibition (RHOA activation) / compact myelin / ganglioside GT1b binding / central nervous system myelination / sialic acid binding / myelin sheath adaxonal region / positive regulation of myelination / central nervous system myelin formation / negative regulation of axon extension ...mesaxon / Axonal growth inhibition (RHOA activation) / compact myelin / ganglioside GT1b binding / central nervous system myelination / sialic acid binding / myelin sheath adaxonal region / positive regulation of myelination / central nervous system myelin formation / negative regulation of axon extension / cell-cell adhesion via plasma-membrane adhesion molecules / paranode region of axon / Schmidt-Lanterman incisure / positive regulation of astrocyte differentiation / axon regeneration / transmission of nerve impulse / negative regulation of neuron differentiation / myelination / cellular response to mechanical stimulus / negative regulation of neuron projection development / myelin sheath / carbohydrate binding / negative regulation of neuron apoptotic process / cell adhesion / membrane raft / signaling receptor binding / protein kinase binding / protein homodimerization activity / plasma membrane Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.3 Å | |||||||||
Authors | Pronker, M.F. / Janssen, B.J.C. | |||||||||
Funding support | Netherlands, 1items
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Citation | Journal: Nat Commun / Year: 2016 Title: Structural basis of myelin-associated glycoprotein adhesion and signalling. Authors: Pronker, M.F. / Lemstra, S. / Snijder, J. / Heck, A.J. / Thies-Weesie, D.M. / Pasterkamp, R.J. / Janssen, B.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5lfu.cif.gz | 220.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5lfu.ent.gz | 179.4 KB | Display | PDB format |
PDBx/mmJSON format | 5lfu.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lf/5lfu ftp://data.pdbj.org/pub/pdb/validation_reports/lf/5lfu | HTTPS FTP |
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-Related structure data
Related structure data | 5lf5C 5lfrC 5lfvC 1cs6S 1urlS 2yd6S 3p3yS 4frwS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 55192.984 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mag / Plasmid: pUPE107.03 / Cell line (production host): HEK293 / Organ (production host): Kidney / Production host: Homo sapiens (human) / Variant (production host): GntI-/- and EBNA1-expressing / References: UniProt: P20917 |
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-Sugars , 5 types, 7 molecules
#2: Polysaccharide | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
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#3: Polysaccharide | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#4: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source |
#5: Sugar | ChemComp-MAN / |
#6: Sugar |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 7.9 Å3/Da |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: Crystals were grown from protein that was modified by reductive lysine methylation. Protein was concentrated to 8.4 mg/mL, which was mixed 1:1 with reservoir solution. Crystals grew in a ...Details: Crystals were grown from protein that was modified by reductive lysine methylation. Protein was concentrated to 8.4 mg/mL, which was mixed 1:1 with reservoir solution. Crystals grew in a condition containing 200 mM NaOAc and 20 % PEG3350 (w/v). PH range: 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P14 (MX2) / Wavelength: 0.97553 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 15, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97553 Å / Relative weight: 1 |
Reflection | Resolution: 4.3→114.62 Å / Num. obs: 15430 / % possible obs: 100 % / Redundancy: 35.7 % / Rmerge(I) obs: 0.115 / Net I/σ(I): 15.6 |
Reflection shell | Resolution: 4.3→4.81 Å / Redundancy: 36.9 % / Rmerge(I) obs: 3.937 / Mean I/σ(I) obs: 1.3 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1URL, 4FRW, 1CS6, 3P3Y, 2YD6 Resolution: 4.3→114.579 Å / SU ML: 0.64 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 42.57 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.3→114.579 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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