PDB-5ldq: Crystal structure of E.coli LigT complexed with NADP+

ID or keywords:

Entry

Database: PDB / ID: 5ldq

Title

Crystal structure of E.coli LigT complexed with NADP+

Components

RNA 2',3'-cyclic phosphodiesterase

Keywords

HYDROLASE / Enzyme / 2H phosphoesterase/ligase

Function / homology

Function and homology information

RNA 2',3'-cyclic 3'-phosphodiesterase / RNA 2',3'-cyclic 3'-phosphodiesterase activity / 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity / ligase activity / ATP binding
Similarity search - Function

Biological species

Escherichia coli BL21 (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 1.7 Å

Authors

Myllykoski, M. / Kursula, P.

Citation

Journal: PLoS ONE / Year: 2017
Title: Structural aspects of nucleotide ligand binding by a bacterial 2H phosphoesterase.
Authors: Myllykoski, M. / Kursula, P.

History

Deposition	Jun 27, 2016	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Jan 18, 2017	Provider: repository / Type: Initial release
Revision 1.1	Feb 8, 2017	Group: Database references
Revision 1.2	Mar 7, 2018	Group: Data collection / Category: diffrn_source / Item: _diffrn_source.pdbx_synchrotron_site
Revision 1.3	Jan 10, 2024	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5ldq.cif.gz	339.3 KB	Display	PDBx/mmCIF format
PDB format	pdb5ldq.ent.gz	282.3 KB	Display	PDB format
PDBx/mmJSON format	5ldq.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/ld/5ldq ftp://data.pdbj.org/pub/pdb/validation_reports/ld/5ldq	HTTPS FTP

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Related structure data

Related structure data	5ldiSC 5ldjC 5ldkC 5ldmC 5ldoC 5ldpC S: Starting model for refinement C: citing same article (ref.)
Similar structure data	1h1d-d 6i3c-d 6ycd-2 6myc-2 5qc3-1 5lqn-d 2z6y-4 4psd-d 6aw9-3 5pa4-d Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Related structure data

5ldiSC

S: Starting model for refinement

C: citing same article (ref.)

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI

Deposited unit

A: RNA 2',3'-cyclic phosphodiesterase

B: RNA 2',3'-cyclic phosphodiesterase

C: RNA 2',3'-cyclic phosphodiesterase

D: RNA 2',3'-cyclic phosphodiesterase

hetero molecules

84.8 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: RNA 2',3'-cyclic phosphodiesterase

hetero molecules

defined by author
21.8 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

B: RNA 2',3'-cyclic phosphodiesterase

hetero molecules

defined by author
21.1 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

C: RNA 2',3'-cyclic phosphodiesterase

hetero molecules

defined by author
21.1 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

D: RNA 2',3'-cyclic phosphodiesterase

hetero molecules

defined by author
20.9 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	86.740, 91.620, 120.610
Angle α, β, γ (deg.)	90.00, 102.31, 90.00
Int Tables number	5
Space group name H-M	C121

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Protein , 1 types, 4 molecules ABCD

#1: Protein	RNA 2',3'-cyclic phosphodiesterase / RNA 2' / 3'-CPDase Mass: 20045.029 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli BL21(DE3) (bacteria) / Gene: thpR, ECBD_3472 / Plasmid: pTH 27 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: A0A140NFI1, Hydrolases; Acting on ester bonds; Phosphoric-diester hydrolases

#1: Protein

RNA 2',3'-cyclic phosphodiesterase / RNA 2' / 3'-CPDase

Mass: 20045.029 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Escherichia coli BL21(DE3) (bacteria) / Gene: thpR, ECBD_3472 / Plasmid: pTH 27 / Production host:

Escherichia coli BL21(DE3) (bacteria)
References: UniProt: A0A140NFI1, Hydrolases; Acting on ester bonds; Phosphoric-diester hydrolases

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Non-polymers , 5 types, 650 molecules

#2: Chemical	ChemComp-NAP / NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / 2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE Mass: 743.405 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C₂₁H₂₈N₇O₁₇P₃
#3: Chemical	ChemComp-CL / CHLORIDE ION Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
#4: Chemical	ChemComp-1PE / PENTAETHYLENE GLYCOL / PEG400 Mass: 238.278 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C₁₀H₂₂O₆ / Comment: precipitant*YM
#5: Chemical	ChemComp-PO4 / PHOSPHATE ION Mass: 94.971 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: PO₄
#6: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 638 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.92 Å³/Da / Density % sol: 57.87 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.4 Details: 0.1 M Tris-HCl pH 7.4, 0.2 M MgCl2, 20% (w/v) PEG 8000

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 1.03 Å
Detector	Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 19, 2013
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1.03 Å / Relative weight: 1
Reflection	Resolution: 1.7→120 Å / Num. obs: 100969 / % possible obs: 99.8 % / Redundancy: 3.8 % / B_iso Wilson estimate: 27.3 Å² / CC_1/2: 0.999 / R_merge(I) obs: 0.053 / Net I/σ(I): 13.92
Reflection shell	Resolution: 1.7→1.74 Å / Redundancy: 3.86 % / R_merge(I) obs: 1.043 / Mean I/σ(I) obs: 1.23 / CC_1/2: 0.619 / % possible all: 99.8

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Processing

Software

Name	Version	Classification
PHENIX	1.8.2_1309	refinement
XDS		data reduction
XSCALE		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: PDBid:5ldi

5ldi

Resolution: 1.7→117.835 Å / SU ML: 0.24 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 28.5

	R_factor	Num. reflection	% reflection	Selection details
R_free	0.2126	1998	1.98 %	Random selection
R_work	0.1834	-	-	-
obs	0.1839	100858	99.71 %	-

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Displacement parameters

B_iso mean: 39.45 Å²

Refinement step

Cycle: LAST / Resolution: 1.7→117.835 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	5555	0	255	638	6448

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.007	6635
X-RAY DIFFRACTION	f_angle_d	1.321	9118
X-RAY DIFFRACTION	f_dihedral_angle_d	19.623	2568
X-RAY DIFFRACTION	f_chiral_restr	0.09	949
X-RAY DIFFRACTION	f_plane_restr	0.004	1165

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
1.6999-1.7424	0.4166	139	0.3832	6928	X-RAY DIFFRACTION	99
1.7424-1.7896	0.4204	143	0.3357	7020	X-RAY DIFFRACTION	100
1.7896-1.8422	0.3442	142	0.3005	7038	X-RAY DIFFRACTION	100
1.8422-1.9017	0.3224	142	0.2745	7022	X-RAY DIFFRACTION	100
1.9017-1.9697	0.3082	144	0.248	7113	X-RAY DIFFRACTION	100
1.9697-2.0485	0.2419	142	0.2099	7019	X-RAY DIFFRACTION	100
2.0485-2.1418	0.2572	141	0.194	7002	X-RAY DIFFRACTION	100
2.1418-2.2547	0.2237	144	0.1836	7118	X-RAY DIFFRACTION	100
2.2547-2.396	0.2178	142	0.1845	7045	X-RAY DIFFRACTION	100
2.396-2.581	0.2117	143	0.1869	7070	X-RAY DIFFRACTION	100
2.581-2.8407	0.2287	143	0.1828	7091	X-RAY DIFFRACTION	100
2.8407-3.2518	0.2034	143	0.1734	7067	X-RAY DIFFRACTION	100
3.2518-4.097	0.1701	144	0.1528	7140	X-RAY DIFFRACTION	100
4.097-118.1188	0.1701	146	0.1566	7187	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.2057	-0.3455	0.771	3.6364	-3.5796	5.6296	0.2454	-0.2989	0.1797	0.2328	-0.0792	0.3445	0.2793	-0.2897	-0.1426	0.1826	-0.1212	0.0554	0.2125	-0.04	0.2321	11.5576	-9.9389	54.9224
2	7.7649	5.0426	2.2719	3.4653	1.2296	3.622	-0.4358	0.2024	0.4761	-0.8441	0.3533	1.0516	-0.0688	-0.6162	0.1542	0.2187	-0.0431	-0.1119	0.2685	0.0248	0.2625	5.6166	-14.2737	33.8644
3	2.8403	1.65	2.2983	2.1839	1.8786	3.9895	0.1595	-0.1963	0.106	0.1855	-0.2035	0.2884	0.2217	-0.389	0.0482	0.2024	-0.0865	0.0169	0.1964	0.0047	0.2084	6.6227	-17.8292	48.575
4	3.4506	3.0922	-2.8267	8.0518	-0.23	4.8009	0.4537	-0.7978	0.2154	0.8741	-0.3372	-0.2282	-0.1835	0.3108	-0.2126	0.1667	-0.0678	-0.0832	0.3594	-0.0116	0.2391	21.0934	-4.6694	63.1323
5	0.8646	0.1592	0.0585	1.6111	0.2928	3.1267	-0.1242	-0.0268	-0.2017	-0.227	0.0824	-0.1852	0.3664	0.0727	0.027	0.1576	0.024	-0.0038	0.1467	0.0146	0.1839	18.2938	-19.7224	41.9509
6	5.0194	-0.9164	-3.3091	7.1935	1.0918	2.3947	0.1198	-0.3649	-0.1146	0.7981	-0.0542	-0.5179	0.6388	1.1036	-0.0559	0.2619	0.0092	-0.0934	0.2459	0.023	0.2739	26.0906	-14.5148	54.6441
7	0.2	-0.2153	-0.322	0.7114	0.7904	0.9194	0.3924	-1.2339	-0.1612	1.2966	-0.1199	-0.123	1.741	0.2194	-0.2313	0.7055	-0.0681	-0.0821	0.605	0.0404	0.3175	20.3009	-22.0758	55.5191
8	7.0984	3.906	1.9419	7.1543	2.3862	3.1546	0.0408	-0.3207	-0.6243	0.5059	-0.2117	-0.1506	1.1602	-0.4041	0.1997	0.4171	-0.1335	0.0275	0.2691	0.0216	0.2376	5.0284	-25.6146	45.0523
9	0.3513	-0.1823	0.1746	2.6613	0.5956	1.8689	0.0153	-0.1157	-0.0941	-0.0982	-0.2286	0.2245	0.4633	-0.6053	0.1132	0.184	-0.139	0.0168	0.3385	-0.026	0.2182	10.811	-13.948	42.6998
10	6.8256	1.9214	-0.3808	6.0403	0.6652	3.249	0.1335	0.0738	0.2648	-0.1011	-0.0425	0.1538	0.189	-0.2426	-0.0395	0.0986	-0.0278	-0.0561	0.2133	0.0297	0.166	14.8896	-4.6028	50.6209
11	4.0105	0.2001	1.2095	2.5291	0.7644	3.4105	0.0532	0.0675	0.7842	0.275	0.0338	0.0802	-0.9037	0.0415	-0.0028	0.3289	-0.0152	0.0803	0.1639	0.0276	0.3578	-1.7425	-19.9223	76.9546
12	5.1907	-1.2927	-5.0929	2.8974	2.2132	6.5885	0.0055	-0.3106	0.6943	0.4407	0.135	-0.8338	0.1145	0.8177	-0.1226	0.0605	0.007	-0.1063	0.307	0.0494	0.375	11.5544	-37.4936	74.4543
13	3.1698	-2.9956	-3.436	4.1274	1.6611	5.8552	0.4239	-0.5052	0.2473	-0.8779	0.0066	-1.3695	-0.6045	1.0781	-0.3001	0.1734	-0.0327	0.1033	0.4823	0.0615	0.4577	15.1856	-28.9556	65.1989
14	7.0678	-2.6334	-0.0479	7.466	1.2906	1.8451	-0.0175	0.3114	0.3654	0.4078	-0.0538	0.05	-0.8117	0.2483	0.0814	0.3288	-0.028	0.0261	0.1761	0.0808	0.1865	-0.4622	-21.6554	74.0405
15	7.0057	5.069	-1.6765	7.8565	-2.0652	7.6468	0.3675	0.1072	1.5813	-0.1	0.0886	1.0231	-1.1226	-0.3058	-0.4019	0.4241	0.1594	0.0198	0.2819	-0.0089	0.5075	-13.5274	-14.9208	81.3227
16	2.0841	-2.3156	-0.8945	2.9265	1.1956	0.4708	-0.0941	0.1636	-0.1678	-0.3045	-0.118	0.2609	-0.1508	-0.5566	0.0069	-0.0625	0.1053	-0.0491	0.3166	-0.023	0.2983	-9.421	-35.8971	77.4545
17	6.4013	4.4897	-2.2602	5.0696	0.1201	2.595	-0.019	0.1992	0.0637	-0.7107	0.0281	0.2127	-0.306	-0.0901	-0.0668	0.1763	0.0489	-0.0663	0.1943	0.0042	0.2167	0.7826	-34.0861	62.9659
18	8.2602	-4.3413	-0.7009	4.2765	-1.4279	8.0137	0.0717	0.6257	0.4912	-0.4347	-0.152	0.9853	-0.5677	-1.3784	-0.0093	0.255	0.1255	-0.0424	0.4001	0.0498	0.2733	-14.1617	-26.6067	71.9789
19	7.02	-4.1471	-3.1386	4.5731	3.8914	3.3808	0.3687	0.5928	1.0267	-0.5466	-0.3278	0.262	-2.1225	-0.8785	-0.1758	0.6425	0.2012	0.026	0.4435	0.0224	0.4358	-8.7033	-24.3076	66.5038
20	7.8671	1.4376	-4.4533	5.5485	0.6926	3.0422	0.2166	0.288	0.31	-0.952	0.0826	-0.3974	-0.6941	0.0774	-0.3089	0.3147	-0.023	0.0641	0.2587	0.0594	0.2719	9.5975	-27.8978	62.4993
21	1.5287	-0.661	-1.0969	3.3295	-0.2704	2.319	0.0549	0.0129	0.0684	0.0566	-0.1015	-0.3421	-0.2349	0.1722	0.0538	0.1235	-0.005	-0.0629	0.2005	0.0246	0.2136	3.4587	-31.3668	73.8633
22	2.8067	4.6922	-2.8578	8.9476	-2.9848	7.4838	-0.0896	-0.0484	-0.0293	0.1163	0.0733	-0.0544	-0.4128	0.0117	0.0337	0.1602	0.0771	-0.0066	0.1809	-0.0074	0.1461	-4.3327	-24.6574	82.6045
23	2.19	-1.2105	1.0037	4.547	-0.6797	2.7702	-0.1195	0.0937	-0.1828	-0.2997	0.1432	0.4167	0.5915	-0.2959	-0.0837	0.3112	-0.0798	0.0022	0.2316	-0.0063	0.2185	13.3886	-16.3725	13.4645
24	6.691	4.5864	0.7223	4.1278	1.0687	0.4375	0.327	-0.3924	-0.887	0.4149	-0.0778	-0.5445	1.6704	0.2096	-0.2338	1.1004	0.1309	0.031	0.3049	0.0368	0.4351	24.8435	-33.4431	26.8605
25	1.3886	-0.8229	-0.0358	1.8775	-0.4523	0.1344	0.0342	0.0683	-0.0507	-0.7045	-0.1314	-0.1842	0.4486	0.1163	-0.066	0.3643	0.0036	0.0193	0.1885	-0.0041	0.2112	24.5159	-12.9846	10.1873
26	5.0347	-1.2927	0.8577	2.5252	1.1205	5.1046	-0.3799	0.2427	-0.6879	-0.2559	0.1456	-1.3666	1.0927	1.4502	0.0233	0.4289	0.197	0.1216	0.5004	0.0123	0.375	31.8399	-23.018	19.0279
27	2.7934	0.5257	0.7415	0.8703	-0.2754	4.6812	0.0446	0.1847	-0.511	-0.9129	0.0427	0.0793	0.7037	0.446	-0.0863	0.501	0.0086	0.0259	0.2435	-0.0356	0.2818	20.7943	-15.9013	7.1216
28	0.7244	-0.6889	-1.1047	3.5021	0.6541	2.9244	-0.1088	0.2437	-0.043	-0.5272	-0.0212	0.6109	0.5991	-0.2953	0.1122	0.4304	-0.1009	-0.0403	0.2142	-0.0214	0.27	15.0195	-20.3608	19.692
29	8.4462	2.1837	2.8334	6.4683	1.2019	6.6929	-0.1435	0.2981	0.1843	-0.0583	-0.0157	0.4158	0.3545	0.0315	0.1496	0.2525	-0.0188	0.0336	0.1519	-0.0105	0.1483	18.2237	-21.1177	25.2275
30	2.8611	-0.4302	-1.5133	3.8064	1.6312	4.9529	0.165	0.1435	0.0744	-0.6198	-0.3408	0.3579	-0.2399	-0.8319	0.183	0.2778	0.0248	-0.0498	0.301	-0.0228	0.2135	-26.0702	-29.7002	12.594
31	8.772	1.8719	3.1701	4.0946	-1.7725	3.2654	0.1304	0.5671	-0.2515	-1.6949	-0.0925	-0.778	0.3015	0.9178	0.0221	0.7484	0.0378	0.2849	0.3369	-0.0103	0.431	-10.1323	-42.6628	-0.5261
32	1.9285	0.1065	-0.622	2.1333	1.7486	2.8242	0.0842	-0.138	0.0998	-0.1957	0.0035	-0.26	-0.6091	0.0598	-0.0152	0.2153	-0.082	0.0001	0.2374	0.0159	0.2062	-15.8218	-27.7982	19.4221
33	6.0029	-1.0121	1.8586	8.781	-0.1662	3.4766	-0.1797	0.6987	-0.0489	-0.9349	0.0083	-1.4221	-0.608	1.9074	0.1392	0.2463	-0.1646	0.0952	0.4808	0.019	0.5889	-6.0809	-34.4144	10.667
34	3.4418	-0.82	-1.7884	0.7391	1.6473	3.8051	0.5659	1.7309	1.2153	-2.557	-0.0004	-1.4635	-1.2375	0.8561	-0.4235	0.9517	-0.0828	0.3038	0.749	0.1538	0.6833	-10.2069	-26.4399	8.4963
35	1.3913	-0.0473	-0.4297	4.1202	1.472	4.5021	0.1447	-0.0255	0.1862	-0.4072	-0.1962	0.2386	-0.5881	-0.664	0.065	0.2676	0.0405	0.0022	0.2708	-0.0073	0.2154	-24.6232	-27.5811	15.1023
36	7.557	-2.6897	3.5889	2.9478	-2.7742	8.2732	-0.2616	-0.0301	0.2188	0.0078	-0.042	-0.1967	-0.1096	-0.311	0.057	0.2719	-0.0439	-0.0437	0.2312	0.002	0.2366	-21.353	-40.1746	8.0082

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 2 through 14 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 15 through 28 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 29 through 53 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 54 through 66 )
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 67 through 96 )
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 97 through 112 )
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 113 through 125 )
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 126 through 134 )
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 135 through 162 )
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 163 through 176 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 3 through 14 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 15 through 28 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 29 through 38 )
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 39 through 53 )
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 54 through 66 )
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 67 through 77 )
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 78 through 96 )
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 97 through 111 )
19	X-RAY DIFFRACTION	19	chain 'B' and (resid 112 through 125 )
20	X-RAY DIFFRACTION	20	chain 'B' and (resid 126 through 134 )
21	X-RAY DIFFRACTION	21	chain 'B' and (resid 135 through 162 )
22	X-RAY DIFFRACTION	22	chain 'B' and (resid 163 through 176 )
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 2 through 53 )
24	X-RAY DIFFRACTION	24	chain 'C' and (resid 54 through 66 )
25	X-RAY DIFFRACTION	25	chain 'C' and (resid 67 through 96 )
26	X-RAY DIFFRACTION	26	chain 'C' and (resid 97 through 112 )
27	X-RAY DIFFRACTION	27	chain 'C' and (resid 113 through 145 )
28	X-RAY DIFFRACTION	28	chain 'C' and (resid 146 through 162 )
29	X-RAY DIFFRACTION	29	chain 'C' and (resid 163 through 176 )
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 2 through 53 )
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 54 through 66 )
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 67 through 96 )
33	X-RAY DIFFRACTION	33	chain 'D' and (resid 97 through 112 )
34	X-RAY DIFFRACTION	34	chain 'D' and (resid 113 through 125 )
35	X-RAY DIFFRACTION	35	chain 'D' and (resid 126 through 162 )
36	X-RAY DIFFRACTION	36	chain 'D' and (resid 163 through 176 )

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