+Open data
-Basic information
Entry | Database: PDB / ID: 5l6w | ||||||
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Title | Structure Of the LIMK1-ATPgammaS-CFL1 Complex | ||||||
Components |
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Keywords | TRANSFERASE / kinase complex | ||||||
Function / homology | Function and homology information actin filament fragmentation / positive regulation of embryonic development / establishment of spindle localization / positive regulation by host of viral process / positive regulation of actin filament bundle assembly / actin filament severing / regulation of dendritic spine morphogenesis / negative regulation of ubiquitin-protein transferase activity / actin filament depolymerization / RHO GTPases Activate ROCKs ...actin filament fragmentation / positive regulation of embryonic development / establishment of spindle localization / positive regulation by host of viral process / positive regulation of actin filament bundle assembly / actin filament severing / regulation of dendritic spine morphogenesis / negative regulation of ubiquitin-protein transferase activity / actin filament depolymerization / RHO GTPases Activate ROCKs / axon extension / Sema4D induced cell migration and growth-cone collapse / regulation of cell morphogenesis / Fc-gamma receptor signaling pathway involved in phagocytosis / stress fiber assembly / RHO GTPases activate PAKs / lamellipodium membrane / mitotic cytokinesis / Rho protein signal transduction / Sema3A PAK dependent Axon repulsion / positive regulation of axon extension / cytoskeleton organization / positive regulation of stress fiber assembly / EPHB-mediated forward signaling / heat shock protein binding / Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation / male germ cell nucleus / response to virus / Regulation of actin dynamics for phagocytic cup formation / ruffle membrane / nuclear matrix / actin filament binding / actin cytoskeleton / Platelet degranulation / lamellipodium / nervous system development / growth cone / actin cytoskeleton organization / vesicle / cytoskeleton / non-specific serine/threonine protein kinase / neuron projection / protein kinase activity / nuclear speck / protein phosphorylation / focal adhesion / protein serine kinase activity / protein serine/threonine kinase activity / negative regulation of apoptotic process / signal transduction / extracellular space / extracellular exosome / ATP binding / membrane / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.53 Å | ||||||
Authors | Salah, E. / Mathea, S. / Oerum, S. / Newman, J.A. / Tallant, C. / Adamson, R. / Canning, P. / Beltrami, A. / von Delft, F. / Arrowsmith, C.H. ...Salah, E. / Mathea, S. / Oerum, S. / Newman, J.A. / Tallant, C. / Adamson, R. / Canning, P. / Beltrami, A. / von Delft, F. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Knapp, S. / Bullock, A.N. | ||||||
Citation | Journal: To Be Published Title: Structure Of the LIMK1-ATPgammaS-CFL1 Complex Authors: Salah, E. / Bullock, A.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5l6w.cif.gz | 103 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5l6w.ent.gz | 75.1 KB | Display | PDB format |
PDBx/mmJSON format | 5l6w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/l6/5l6w ftp://data.pdbj.org/pub/pdb/validation_reports/l6/5l6w | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 35994.773 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: LIMK1, LIMK / Production host: Spodoptera frugiperda (fall armyworm) References: UniProt: P53667, non-specific serine/threonine protein kinase |
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#2: Protein | Mass: 18635.674 Da / Num. of mol.: 1 / Mutation: S3C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CFL1, CFL / Production host: Escherichia coli (E. coli) / References: UniProt: P23528 |
#3: Chemical | ChemComp-AGS / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.09 Å3/Da / Density % sol: 69.9 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 35% pentaerythritol propoxylate 5/4, 0.1 M HEPES pH 7.5, 0.2 M potassium chloride |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.97949 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 15, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97949 Å / Relative weight: 1 |
Reflection | Resolution: 2.53→34.94 Å / Num. obs: 30888 / % possible obs: 100 % / Redundancy: 12.2 % / Rmerge(I) obs: 0.061 / Net I/σ(I): 19.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1Q8G, 3S95 Resolution: 2.53→34.94 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.922 / SU B: 12.737 / SU ML: 0.254 / Cross valid method: THROUGHOUT / ESU R: 0.27 / ESU R Free: 0.249 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 88.725 Å2
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Refinement step | Cycle: 1 / Resolution: 2.53→34.94 Å
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