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- PDB-5ku6: Crystal structure for the complex of human carbonic anhydrase IV ... -

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Basic information

Entry
Database: PDB / ID: 5ku6
TitleCrystal structure for the complex of human carbonic anhydrase IV and methazolamide
ComponentsCarbonic anhydrase 4
Keywordslyase/lyase inhibitor / Carbonic anhydrase / Intrinsic thermodynamics / lyase / inhibitor / lyase-lyase inhibitor complex
Function / homology
Function and homology information


bicarbonate transport / transport vesicle membrane / endoplasmic reticulum-Golgi intermediate compartment / rough endoplasmic reticulum / secretory granule membrane / Reversible hydration of carbon dioxide / carbonic anhydrase / carbonate dehydratase activity / brush border membrane / trans-Golgi network ...bicarbonate transport / transport vesicle membrane / endoplasmic reticulum-Golgi intermediate compartment / rough endoplasmic reticulum / secretory granule membrane / Reversible hydration of carbon dioxide / carbonic anhydrase / carbonate dehydratase activity / brush border membrane / trans-Golgi network / Erythrocytes take up oxygen and release carbon dioxide / Erythrocytes take up carbon dioxide and release oxygen / one-carbon metabolic process / apical plasma membrane / external side of plasma membrane / perinuclear region of cytoplasm / Golgi apparatus / cell surface / extracellular exosome / zinc ion binding / membrane / plasma membrane
Similarity search - Function
Carbonic anhydrase, CA4/CA15 / Carbonic Anhydrase II / Alpha carbonic anhydrase / Carbonic anhydrase, alpha-class, conserved site / Alpha-carbonic anhydrases signature. / Carbonic anhydrase, alpha-class / Alpha carbonic anhydrase domain / Alpha carbonic anhydrase domain superfamily / Eukaryotic-type carbonic anhydrase / Alpha-carbonic anhydrases profile. ...Carbonic anhydrase, CA4/CA15 / Carbonic Anhydrase II / Alpha carbonic anhydrase / Carbonic anhydrase, alpha-class, conserved site / Alpha-carbonic anhydrases signature. / Carbonic anhydrase, alpha-class / Alpha carbonic anhydrase domain / Alpha carbonic anhydrase domain superfamily / Eukaryotic-type carbonic anhydrase / Alpha-carbonic anhydrases profile. / Eukaryotic-type carbonic anhydrase / Roll / Alpha Beta
Similarity search - Domain/homology
ACETATE ION / Chem-MZM / Carbonic anhydrase 4
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.8 Å
AuthorsChen, Z. / Waheed, A. / Di Cera, E. / Sly, W.S.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL049413 United States
National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)HL073813 United States
Citation
Journal: Eur. Biophys. J. / Year: 2018
Title: Intrinsic thermodynamics of high affinity inhibitor binding to recombinant human carbonic anhydrase IV.
Authors: Mickeviciute, A. / Timm, D.D. / Gedgaudas, M. / Linkuviene, V. / Chen, Z. / Waheed, A. / Michailoviene, V. / Zubriene, A. / Smirnov, A. / Capkauskaite, E. / Baranauskiene, L. / Jachno, J. / ...Authors: Mickeviciute, A. / Timm, D.D. / Gedgaudas, M. / Linkuviene, V. / Chen, Z. / Waheed, A. / Michailoviene, V. / Zubriene, A. / Smirnov, A. / Capkauskaite, E. / Baranauskiene, L. / Jachno, J. / Revuckiene, J. / Manakova, E. / Grazulis, S. / Matuliene, J. / Di Cera, E. / Sly, W.S. / Matulis, D.
#1: Journal: PNAS / Year: 1996
Title: Crystal structure of the secretory form of membrane-associated human carbonic anhydrase IV at 2.8 A resolution
Authors: Stams, T. / Nair, S.K. / Okuyama, T. / Waheed, A. / Sly, W.S. / Christianson, W.
History
DepositionJul 12, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 26, 2017Provider: repository / Type: Initial release
Revision 1.1Aug 9, 2017Group: Database references / Category: pdbx_related_exp_data_set
Revision 1.2Feb 6, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.3Mar 23, 2022Group: Author supporting evidence / Database references / Derived calculations
Category: database_2 / pdbx_audit_support / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id
Revision 1.4Oct 4, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.5Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Carbonic anhydrase 4
B: Carbonic anhydrase 4
C: Carbonic anhydrase 4
D: Carbonic anhydrase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)124,07128
Polymers121,4584
Non-polymers2,61324
Water16,484915
1
A: Carbonic anhydrase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,9506
Polymers30,3641
Non-polymers5865
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Carbonic anhydrase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)31,1028
Polymers30,3641
Non-polymers7377
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
3
C: Carbonic anhydrase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)31,1028
Polymers30,3641
Non-polymers7377
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
4
D: Carbonic anhydrase 4
hetero molecules


Theoretical massNumber of molelcules
Total (without water)30,9176
Polymers30,3641
Non-polymers5535
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
5


  • Idetical with deposited unit
  • defined by software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8900 Å2
ΔGint-265 kcal/mol
Surface area45940 Å2
MethodPISA
Unit cell
Length a, b, c (Å)64.965, 123.698, 151.367
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
Carbonic anhydrase 4 / Carbonate dehydratase IV / Carbonic anhydrase IV / CA-IV


Mass: 30364.473 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CA4 / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: P22748, carbonic anhydrase

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Non-polymers , 6 types, 939 molecules

#2: Chemical
ChemComp-MZM / N-(3-methyl-5-sulfamoyl-1,3,4-thiadiazol-2(3H)-ylidene)acetamide / Methazolamide


Mass: 236.272 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C5H8N4O3S2 / Comment: inhibitor*YM
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#4: Chemical
ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: SO4
#5: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C3H8O3
#6: Chemical ChemComp-ACT / ACETATE ION


Mass: 59.044 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C2H3O2
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 915 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.5 Å3/Da / Density % sol: 50.87 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 4.6 / Details: 100 mM Na acetate, 200 mM AmSO4 and 23% PEG 4000

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.5418 Å
DetectorType: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Feb 26, 2015
RadiationMonochromator: mirror / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.5418 Å / Relative weight: 1
ReflectionResolution: 1.8→40 Å / Num. obs: 110310 / % possible obs: 97.3 % / Observed criterion σ(F): -0.5 / Observed criterion σ(I): -0.5 / Redundancy: 4.4 % / Rmerge(I) obs: 0.089 / Net I/σ(I): 11.4
Reflection shellResolution: 1.8→1.83 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.422 / Mean I/σ(I) obs: 2.1 / % possible all: 94.2

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Processing

Software
NameVersionClassification
REFMAC5.5.0109refinement
HKL-2000data reduction
HKL-2000data scaling
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1ZNC

1znc


Resolution: 1.8→95.78 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.939 / SU B: 6.494 / SU ML: 0.091 / Cross valid method: THROUGHOUT / ESU R: 0.128 / ESU R Free: 0.12 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.21585 5481 5 %RANDOM
Rwork0.18416 ---
obs0.18574 104356 96.85 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK
Displacement parametersBiso mean: 22.503 Å2
Baniso -1Baniso -2Baniso -3
1--0.07 Å20 Å20 Å2
2---0.06 Å20 Å2
3---0.13 Å2
Refinement stepCycle: LAST / Resolution: 1.8→95.78 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8360 0 142 915 9417
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0090.0228698
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg1.3611.9711755
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg6.27151027
X-RAY DIFFRACTIONr_dihedral_angle_2_deg33.51224.938403
X-RAY DIFFRACTIONr_dihedral_angle_3_deg14.423151551
X-RAY DIFFRACTIONr_dihedral_angle_4_deg11.1371536
X-RAY DIFFRACTIONr_chiral_restr0.0830.21253
X-RAY DIFFRACTIONr_gen_planes_refined0.0050.0216500
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.4371.55202
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it0.83928403
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it1.52833496
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it2.5454.53350
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 1.801→1.848 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.346 413 -
Rwork0.323 7269 -
obs--92.67 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.0894-1.0446-0.82453.97941.12743.6041-0.0422-0.0454-0.31540.08640.1171-0.19320.24460.2052-0.07490.12940.0203-0.00320.15760.01050.16338.8256-6.817116.7461
23.9842-1.1904-0.42233.17890.39722.1602-0.0712-0.5794-0.03440.28040.14830.1988-0.0044-0.0739-0.07720.1428-0.01520.040.10310.00820.065523.00356.153626.8879
32.4826-2.0167-1.45973.53781.91021.4802-0.0227-0.09810.3034-0.04740.0904-0.1938-0.16090.0898-0.06770.1251-0.0278-0.03960.15180.00040.112532.751321.210612.5218
41.8765-0.6341-0.63055.1242.50382.8724-0.0432-0.08270.2104-0.02640.1093-0.2176-0.12480.2221-0.06620.0595-0.0149-0.01470.08110.03370.055532.128616.829215.5413
57.67422.3539-1.56453.35261.20395.6297-0.0840.5498-0.1144-0.3940.11220.19710.1713-0.227-0.02820.1238-0.001-0.02090.08880.02970.082619.54493.660613.7612
62.7252.17511.85265.47133.71655.17220.0422-0.22130.04560.31610.0253-0.13080.00690.0507-0.06740.06070.01330.01560.0570.01560.057730.190916.124121.111
73.70760.22790.28861.73140.30681.36530.03880.19030.3495-0.1609-0.0412-0.0539-0.12320.09950.00240.1536-0.01570.00860.13560.01430.064534.531613.1262-0.385
83.25091.2706-5.21730.8407-1.78138.60770.20410.42530.3028-0.18330.08180.3205-0.5095-0.7085-0.28590.27160.0513-0.0780.1615-0.00850.234621.45122.10611.9687
91.1458-0.6934-0.7281.3680.83083.130.05010.0274-0.0055-0.0066-0.013-0.01670.0130.1181-0.03710.0402-0.0321-0.00320.04330.01040.061731.23776.309310.6001
103.2413-2.7762-2.14325.26070.22623.3408-0.3323-0.178-0.38390.58930.11620.71330.208-0.11460.2160.1245-0.03820.06730.1132-0.00740.168521.11166.31926.1491
110.94820.2813.64665.76970.792414.3807-0.22840.18710.04350.20980.2572-0.7049-0.91690.9244-0.02880.1276-0.0463-0.02020.2603-0.10240.290135.61855.7873-21.3238
122.89391.0037-0.10783.2516-0.59382.21-0.09520.43880.0577-0.30980.05280.12780.0686-0.08230.04240.08220.0169-0.02950.0844-0.01450.071923.8284-5.2481-30.8916
135.4956-2.01992.23913.959-0.71412.66490.14860.1657-0.5715-0.0792-0.00450.3310.22960.0413-0.14410.2057-0.01140.05130.1159-0.01610.186120.7995-25.9249-20.9418
141.64651.29341.07972.74891.86771.3175-0.00430.156-0.46540.04090.2787-0.35660.05720.2148-0.27440.23240.04270.01820.14410.01540.204335.7965-19.8222-18.3316
151.11260.85541.23561.93770.33481.6850.0114-0.0234-0.01270.08920.02070.00450.0308-0.0339-0.03210.12510.0178-0.00650.05570.01310.048325.9032-11.4376-17.6923
162.297-0.4371-0.11133.09091.78695.52450.03560.0402-0.0648-0.0372-0.03150.06580.046-0.2312-0.00410.0648-0.0114-0.03080.01260.0060.191526.5723-17.7951-18.353
171.90720.5071.8961.60840.03812.99150.1587-0.2252-0.21680.233-0.0570.01050.3008-0.0252-0.10160.13430.05160.01720.1202-0.01210.15530.5535-16.3727-13.66
182.15510.84132.28531.03490.82996.48980.0725-0.2785-0.10160.1782-0.25360.13870.015-0.73350.18110.1326-0.00560.00430.1246-0.05010.136722.6484-13.8262-14.6315
195.7771.29781.20112.68040.82060.95870.0684-0.13940.07250.0626-0.0515-0.0938-0.0480.2851-0.01690.0659-0.0205-0.02790.1864-0.01490.029336.1384-3.0271-7.4144
202.44080.54021.32252.01460.74083.0585-0.16840.02860.1867-0.1378-0.03440.274-0.2606-0.09040.20280.06970.01970.0140.0560.00770.085224.5271-4.7612-22.6164
215.9889-0.1896-1.22642.7671-2.496710.07510.1220.00960.6555-0.0542-0.1442-0.4019-0.68430.77040.02220.16-0.0457-0.00990.1725-0.00380.203820.249420.9212-18.0121
224.29620.79430.32872.55810.32842.00870.0771-0.3005-0.39110.1982-0.11190.17390.2056-0.2440.03480.11570.00680.03160.11080.02850.10232.54043.9256-11.6108
232.89120.1301-1.13450.6406-0.32751.58730.02560.0050.06940.0234-0.02270.2418-0.1258-0.2117-0.00290.10860.0182-0.01020.1039-0.03190.1081-3.858317.9748-24.085
243.6337-0.6462-1.76461.55660.01613.45590.1319-0.10360.04690.0405-0.03630.0493-0.0486-0.0941-0.09560.058-0.0414-0.02490.0511-0.00550.0638-0.861610.6176-17.8142
2515.4137-0.9921-12.16630.10421.394119.31990.0223-0.50140.13740.02550.0327-0.01110.1210.1732-0.05490.207-0.03220.03470.1948-0.00520.2218-7.469513.5642-2.3676
261.16220.5059-0.38471.2887-1.79122.8699-0.06380.2935-0.1852-0.26760.0402-0.01230.2494-0.13260.02350.14960.0687-0.0260.2048-0.06630.1464-0.189311.8523-32.7594
273.69752.0771-0.34194.2525-0.9091.7556-0.12250.28170.0045-0.34960.1630.27880.0521-0.2935-0.04040.12860.05090.00490.154-0.03590.0856-1.824520.3074-32.955
288.6257-4.9060.21545.1108-5.308515.1210.14160.2825-0.8753-0.49670.14430.60690.7045-0.7353-0.28590.1183-0.0695-0.02840.1049-0.06080.2223-7.07540.4953-21.7298
292.35111.1209-1.49511.3317-1.40384.1021-0.04940.052-0.0356-0.02410.02210.02340.0476-0.11360.02730.01920.0206-0.00350.0315-0.01340.085.720413.8432-24.4607
303.00730.8879-0.47472.5214-0.97542.5062-0.022-0.1401-0.27510.0931-0.1159-0.17010.04740.16180.13790.05720.01720.00240.0542-0.0210.078110.459110.435-18.2114
319.83212.25863.09912.2193-1.796811.05940.35950.4461-0.9831-0.163-0.1717-0.52470.88531.2925-0.18780.22490.1044-0.00040.2355-0.06150.273119.6563-23.843318.6906
322.9110.21020.09212.5136-0.28742.39770.12670.54090.1092-0.6185-0.1766-0.0081-0.07740.05830.04990.19060.0385-0.00240.21760.01640.05087.0772-6.74210.1714
332.6526-1.94430.90612.942-0.81211.2108-0.00440.015-0.0797-0.0074-0.00440.2318-0.0413-0.16720.00880.1065-0.0174-0.01580.0977-0.04120.1042-7.1344-14.624726.2519
343.3771-2.08841.61483.2327-1.47435.1630.05910.1307-0.3475-0.02180.11940.41190.1639-0.2211-0.17840.0474-0.0106-0.0140.0692-0.0370.1398-11.1054-16.489219.7169
352.0052-1.19720.58551.1533-1.03431.30710.06510.1364-0.1369-0.0089-0.04960.0391-0.0240.0424-0.01550.06040.02010.0120.0758-0.02840.05544.0244-11.516122.4248
362.84570.78013.77441.3172.07319.2961-0.01760.1808-0.0221-0.1615-0.0010.0649-0.0882-0.04850.01860.07720.01990.00590.04020.01450.102-2.9447-9.880719.6786
374.4634-2.4154-1.29947.10241.21362.40670.0741-0.1778-0.08620.2686-0.03330.06580.1611-0.0408-0.04070.099-0.0287-0.01440.08510.01110.06512.0191-22.924137.1469
381.27490.8329-2.02675.8189-4.4195.52880.1235-0.15310.23680.84350.06020.2615-0.8274-0.0612-0.18370.20230.05-0.06910.1971-0.0850.2194-6.3849-4.009628.2969
392.3214-0.90341.40410.8731-0.30645.68750.00530.0968-0.1037-0.0692-0.06060.053-0.1832-0.07850.05530.0353-0.01680.00110.0520.01780.07672.975-9.849319.483
402.0307-1.19151.01072.0643-1.07012.56750.08950.1395-0.0552-0.1415-0.0825-0.01420.17530.244-0.00690.0414-0.01590.00480.0726-0.03370.069311.4755-15.238124.8552
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A1 - 15
2X-RAY DIFFRACTION2A16 - 41
3X-RAY DIFFRACTION3A42 - 69
4X-RAY DIFFRACTION4A70 - 101
5X-RAY DIFFRACTION5A102 - 112
6X-RAY DIFFRACTION6A113 - 149
7X-RAY DIFFRACTION7A150 - 178
8X-RAY DIFFRACTION8A179 - 193
9X-RAY DIFFRACTION9A194 - 244
10X-RAY DIFFRACTION10A245 - 259
11X-RAY DIFFRACTION11B4 - 15
12X-RAY DIFFRACTION12B16 - 35
13X-RAY DIFFRACTION13B36 - 55
14X-RAY DIFFRACTION14B56 - 86
15X-RAY DIFFRACTION15B87 - 125
16X-RAY DIFFRACTION16B126 - 156
17X-RAY DIFFRACTION17B157 - 204
18X-RAY DIFFRACTION18B205 - 222
19X-RAY DIFFRACTION19B223 - 233
20X-RAY DIFFRACTION20B236 - 259
21X-RAY DIFFRACTION21C4 - 15
22X-RAY DIFFRACTION22C16 - 46
23X-RAY DIFFRACTION23C47 - 102
24X-RAY DIFFRACTION24C103 - 130
25X-RAY DIFFRACTION25C131 - 140
26X-RAY DIFFRACTION26C141 - 161
27X-RAY DIFFRACTION27C162 - 188
28X-RAY DIFFRACTION28C189 - 192
29X-RAY DIFFRACTION29C193 - 233
30X-RAY DIFFRACTION30C236 - 259
31X-RAY DIFFRACTION31D4 - 15
32X-RAY DIFFRACTION32D16 - 41
33X-RAY DIFFRACTION33D42 - 68
34X-RAY DIFFRACTION34D69 - 91
35X-RAY DIFFRACTION35D92 - 128
36X-RAY DIFFRACTION36D129 - 154
37X-RAY DIFFRACTION37D155 - 178
38X-RAY DIFFRACTION38D179 - 192
39X-RAY DIFFRACTION39D193 - 220
40X-RAY DIFFRACTION40D221 - 259

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