- PDB-5k4m: Directed evolutionary changes in MBL super family - NDM-1 Round 1... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 5k4m
Title
Directed evolutionary changes in MBL super family - NDM-1 Round 10 crystal-3
Components
Metallo-beta-lactamase type 2
Keywords
HYDROLASE / Phosphatase / Directed evolution / MBL super family / NDM-1
Function / homology
Function and homology information
antibiotic catabolic process / beta-lactamase activity / beta-lactamase / periplasmic space / response to antibiotic / zinc ion binding Similarity search - Function
Metallo-beta-lactamasetype2 / B2 metallo-beta-lactamase / Beta-lactamase type II / Metallo-beta-lactamase NDM-1 / Metallo-beta- ...B2 metallo-beta-lactamase / Beta-lactamase type II / Metallo-beta-lactamase NDM-1 / Metallo-beta-lactamase type II / New Delhi metallo-beta-lactamase-1 / NDM-1
Mass: 25988.375 Da / Num. of mol.: 1 / Fragment: UNP residues 29-270 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Klebsiella pneumoniae (bacteria) / Gene: blaNDM-1 / Plasmid: PET28A / Production host: Escherichia coli (E. coli) / References: UniProt: C7C422, beta-lactamase
Resolution: 1.98→39.13 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.919 / SU B: 3.155 / SU ML: 0.092 / Cross valid method: THROUGHOUT / ESU R: 0.165 / ESU R Free: 0.159 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.20775
736
5 %
RANDOM
Rwork
0.14154
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obs
0.14483
13877
99.7 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å