arachidonate 15-lipoxygenase / arachidonate 15-lipoxygenase activity / linoleate 13S-lipoxygenase activity / 酸化還元酵素; 分子状酸素を取り込み一電子供与する; オキシゲナーゼ類; 2分子の酸素を取り込む / lipid oxidation / oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen / fatty acid metabolic process / periplasmic space / extracellular region / metal ion binding 類似検索 - 分子機能
Lipoxygenase, conserved site / Lipoxygenases iron-binding region signature 2. / Lipoxygenase, iron binding site / Lipoxygenases iron-binding region signature 1. / Lipoxygenase / Lipoxygenase, C-terminal / Lipoxigenase, C-terminal domain superfamily / Lipoxygenase / Lipoxygenase iron-binding catalytic domain profile. 類似検索 - ドメイン・相同性
解像度: 1.9→43.8 Å / Cor.coef. Fo:Fc: 0.974 / Cor.coef. Fo:Fc free: 0.966 / SU B: 7.331 / SU ML: 0.091 / 交差検証法: THROUGHOUT / ESU R: 0.521 / ESU R Free: 0.116 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.18195
2586
4.9 %
RANDOM
Rwork
0.14408
-
-
-
obs
0.14593
50471
99.5 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å
原子変位パラメータ
Biso mean: 31.388 Å2
Baniso -1
Baniso -2
Baniso -3
1-
-1.57 Å2
0 Å2
-0 Å2
2-
-
-0.44 Å2
-0 Å2
3-
-
-
2 Å2
精密化ステップ
サイクル: 1 / 解像度: 1.9→43.8 Å
タンパク質
核酸
リガンド
溶媒
全体
原子数
4804
0
67
471
5342
拘束条件
Refine-ID
タイプ
Dev ideal
Dev ideal target
数
X-RAY DIFFRACTION
r_bond_refined_d
0.008
0.019
5086
X-RAY DIFFRACTION
r_bond_other_d
0.002
0.02
4901
X-RAY DIFFRACTION
r_angle_refined_deg
1.224
1.979
6937
X-RAY DIFFRACTION
r_angle_other_deg
0.859
3
11241
LS精密化 シェル
解像度: 1.9→1.949 Å / Total num. of bins used: 20
Rfactor
反射数
%反射
Rfree
0.271
193
-
Rwork
0.241
3704
-
obs
-
-
99.72 %
精密化 TLS
手法: refined / Origin x: 30.688 Å / Origin y: 12.3385 Å / Origin z: 3.1566 Å