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- PDB-5iqn: Crystal structure of the E. coli type 1 pilus subunit FimG (engin... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5iqn | ||||||
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Title | Crystal structure of the E. coli type 1 pilus subunit FimG (engineered variant with substitution Q134E; N-terminal FimG residues 1-12 truncated) in complex with the donor strand peptide DsF_SRIRIRGYVR | ||||||
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![]() | CELL ADHESION / Complex / Protein / FimGt | ||||||
Function / homology | ![]() pilus tip / mechanosensory behavior / cell adhesion involved in single-species biofilm formation / pilus / cell-substrate adhesion / cell adhesion Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Giese, C. / Eras, J. / Kern, A. / Scharer, M.A. / Capitani, G. / Glockshuber, R. | ||||||
![]() | ![]() Title: Accelerating the Association of the Most Stable Protein-Ligand Complex by More than Two Orders of Magnitude. Authors: Giese, C. / Eras, J. / Kern, A. / Scharer, M.A. / Capitani, G. / Glockshuber, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 202.1 KB | Display | ![]() |
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PDB format | ![]() | 165.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446 KB | Display | ![]() |
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Full document | ![]() | 446.8 KB | Display | |
Data in XML | ![]() | 18.7 KB | Display | |
Data in CIF | ![]() | 28.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5iqmC ![]() 5iqoC ![]() 3bfwS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13666.820 Da / Num. of mol.: 2 / Fragment: UNP residues 36-167 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein/peptide | Mass: 1279.540 Da / Num. of mol.: 2 / Fragment: UNP residues 35-34 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() #3: Chemical | #4: Chemical | ChemComp-EDO / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.38 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: MOPS-NaOH pH 6.8 (RT), PEG-1500, CoCl2, NaCl |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Mar 1, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 0.96→50 Å / Num. obs: 133060 / % possible obs: 99.3 % / Redundancy: 5.6 % / Rmerge(I) obs: 0.064 / Rpim(I) all: 0.064 / Net I/σ(I): 12.9 |
Reflection shell | Resolution: 0.96→0.98 Å / Rmerge(I) obs: 1.304 / Rpim(I) all: 1.304 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3bfw Resolution: 1→25.384 Å / SU ML: 0.09 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 13.75 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1→25.384 Å
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Refine LS restraints |
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LS refinement shell |
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