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- PDB-5htn: putative sugar kinases from Synechococcus elongatus PCC7942-apo form -
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Open data
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Basic information
Entry | Database: PDB / ID: 5htn | ||||||
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Title | putative sugar kinases from Synechococcus elongatus PCC7942-apo form | ||||||
![]() | Probable sugar kinase | ||||||
![]() | TRANSFERASE / putative sugar kinases / Synechococcus elongatus PCC7942 / apo form | ||||||
Function / homology | ![]() D-ribulokinase / D-ribulokinase activity / glycerol-3-phosphate biosynthetic process / glycerol kinase activity / glycerol metabolic process / triglyceride metabolic process / ATP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Xie, Y. / Li, M. / Chang, W. | ||||||
![]() | ![]() Title: Crystal Structures of Putative Sugar Kinases from Synechococcus Elongatus PCC 7942 and Arabidopsis Thaliana Authors: Xie, Y. / Li, M. / Chang, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.1 KB | Display | ![]() |
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PDB format | ![]() | 74.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 406.8 KB | Display | ![]() |
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Full document | ![]() | 408 KB | Display | |
Data in XML | ![]() | 18.6 KB | Display | |
Data in CIF | ![]() | 28 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5htjC ![]() 5htpC ![]() 5htrC ![]() 5htvC ![]() 5htxC ![]() 5htyC ![]() 5hu2C ![]() 5huxC ![]() 5hv7C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 47307.238 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: PCC 7942 / Gene: Synpcc7942_2462 / Plasmid: PET28a / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.59 % |
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Crystal grow | Temperature: 281 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.1M BIS-TRIS pH 6.5, 20% w/v Polyethylene glycol monomethyl ether 5000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: Jun 20, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→50 Å / Num. obs: 18837 / % possible obs: 96.1 % / Redundancy: 7.4 % / Net I/σ(I): 13.09 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 7.5 % / Rmerge(I) obs: 0.504 / Mean I/σ(I) obs: 4.1 / % possible all: 94.2 |
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Processing
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Refinement | Resolution: 2.301→32.501 Å / SU ML: 0.24 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.84
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.301→32.501 Å
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Refine LS restraints |
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LS refinement shell |
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