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- PDB-6oga: Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(3... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6oga | ||||||
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Title | Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(3-Br1Y), H148D; circular permutant (50-51) | ||||||
![]() | Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(3-Br1Y), H148D; circular permutant (50-51) | ||||||
![]() | FLUORESCENT PROTEIN / GFP | ||||||
Function / homology | Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / bioluminescence / generation of precursor metabolites and energy / ACETATE ION / Green fluorescent protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lin, C.-Y. / Boxer, S.G. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Unusual Spectroscopic and Electric Field Sensitivity of Chromophores with Short Hydrogen Bonds: GFP and PYP as Model Systems. Authors: Lin, C.Y. / Boxer, S.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 204.7 KB | Display | ![]() |
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PDB format | ![]() | 161.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6og8C ![]() 6og9C ![]() 6ogbC ![]() 6ogcC ![]() 6un5C ![]() 6un6C ![]() 6un7C ![]() 4zf3S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28139.170 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.41 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 50 mM sodium acetate, pH 5.0, 100 mM NaCl, 7-8% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 2, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→34.3 Å / Num. obs: 54833 / % possible obs: 98.33 % / Redundancy: 5.7 % / Biso Wilson estimate: 21.23 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.07353 / Rpim(I) all: 0.03313 / Rrim(I) all: 0.08091 / Net I/σ(I): 13.38 |
Reflection shell | Resolution: 1.6→1.657 Å / Redundancy: 5.8 % / Rmerge(I) obs: 1.092 / Mean I/σ(I) obs: 1.38 / Num. unique obs: 5471 / CC1/2: 0.592 / Rpim(I) all: 0.4877 / Rrim(I) all: 1.199 / % possible all: 98.75 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4zf3 Resolution: 1.6→34.3 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 20.11
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.6→34.3 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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