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- PDB-5h9o: Complex of Murine endoplasmic reticulum alpha-glucosidase II with... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5h9o | ||||||
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Title | Complex of Murine endoplasmic reticulum alpha-glucosidase II with D-Glucose | ||||||
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![]() | HYDROLASE / Enzyme Glycosyl hydrolase GH31 Quality control exoglycosidase | ||||||
Function / homology | ![]() mannosyl-oligosaccharide alpha-1,3-glucosidase / glucan 1,3-alpha-glucosidase activity / glucosidase II complex / glucosidase activity / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Post-translational protein phosphorylation / N-glycan processing / liver development / melanosome / negative regulation of neuron projection development ...mannosyl-oligosaccharide alpha-1,3-glucosidase / glucan 1,3-alpha-glucosidase activity / glucosidase II complex / glucosidase activity / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / Post-translational protein phosphorylation / N-glycan processing / liver development / melanosome / negative regulation of neuron projection development / carbohydrate binding / in utero embryonic development / carbohydrate metabolic process / intracellular membrane-bounded organelle / calcium ion binding / protein-containing complex binding / Golgi apparatus / endoplasmic reticulum / RNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Caputo, A.T. / Roversi, P. / Alonzi, D.S. / Kiappes, J.L. / Zitzmann, N. | ||||||
![]() | ![]() Title: Structures of mammalian ER alpha-glucosidase II capture the binding modes of broad-spectrum iminosugar antivirals. Authors: Caputo, A.T. / Alonzi, D.S. / Marti, L. / Reca, I.B. / Kiappes, J.L. / Struwe, W.B. / Cross, A. / Basu, S. / Lowe, E.D. / Darlot, B. / Santino, A. / Roversi, P. / Zitzmann, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 725.8 KB | Display | ![]() |
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PDB format | ![]() | 611.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 70.6 KB | Display | |
Data in CIF | ![]() | 98.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5f0eC ![]() 5hjoC ![]() 5hjrC ![]() 5iedC ![]() 5ieeC ![]() 5iefC ![]() 5iegC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 2 types, 4 molecules ACBD
#1: Protein | Mass: 97894.625 Da / Num. of mol.: 2 / Fragment: UNP Residues 33-944 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 9568.298 Da / Num. of mol.: 2 / Fragment: UNP residues 30-117 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Sugars , 2 types, 6 molecules ![](data/chem/img/NAG.gif)
![](data/chem/img/GLC.gif)
![](data/chem/img/GLC.gif)
#3: Sugar | ChemComp-NAG / #9: Sugar | |
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-Non-polymers , 8 types, 476 molecules ![](data/chem/img/EDO.gif)
![](data/chem/img/FMT.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/P6G.gif)
![](data/chem/img/OXM.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/TAR.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/FMT.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/P6G.gif)
![](data/chem/img/OXM.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/TAR.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-EDO / #5: Chemical | ChemComp-FMT / #6: Chemical | ChemComp-PG4 / #7: Chemical | ChemComp-P6G / #8: Chemical | ChemComp-OXM / | #10: Chemical | ChemComp-CA / #11: Chemical | ChemComp-TAR / | #12: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.61 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 6.25 Details: 32 % Morpheus ethylene glycol/PEG 8000 mix, 0.05 M Morpheus carboxylic acids mix, 0.1 M Morpheus buffer system 1 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Sep 27, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.37→87.88 Å / Num. obs: 89708 / % possible obs: 99.8 % / Redundancy: 5.5 % / Biso Wilson estimate: 33.37 Å2 / Rsym value: 0.24 / Net I/σ(I): 5.1 |
Reflection shell | Resolution: 2.37→2.43 Å / Redundancy: 5.4 % / Mean I/σ(I) obs: 1.2 / Num. unique all: 6578 / Rsym value: 1.53 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 33.8 Å2
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Refine analyze | Luzzati coordinate error obs: 0.37 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.37→87.66 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.37→2.43 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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