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- PDB-5h9e: Crystal structure of E. coli Cascade bound to a PAM-containing ds... -

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Open data

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Basic information

Entry

Database: PDB / ID: 5h9e

Title

Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target (32-nt spacer) at 3.20 angstrom resolution.

Components

(CRISPR system Cascade subunit ...) x 5
(DNA (47-MER) ...) x 2
crRNA (61-MER)

Keywords

Immune System/RNA / CRISPR Cascade / Immune System-RNA Complex

Function / homology

Function and homology information

CRISPR-cas system / DNA/RNA hybrid binding / maintenance of CRISPR repeat elements / RNA processing / RNA endonuclease activity / defense response to virus / nucleic acid binding / Hydrolases; Acting on ester bonds / protein-containing complex / DNA binding ...
Similarity search - Function

Biological species

Escherichia coli (E. coli)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 3.21 Å

Authors

Hayes, R.P. / Xiao, Y. / Ding, F. / van Erp, P.B.G. / Rajashankar, K. / Bailey, S. / Wiedenheft, B. / Ke, A.

Citation

Journal: Nature / Year: 2016
Title: Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli.
Authors: Hayes, R.P. / Xiao, Y. / Ding, F. / van Erp, P.B. / Rajashankar, K. / Bailey, S. / Wiedenheft, B. / Ke, A.

History

Deposition	Dec 28, 2015	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Feb 17, 2016	Provider: repository / Type: Initial release
Revision 1.1	Feb 24, 2016	Group: Database references
Revision 1.2	Mar 9, 2016	Group: Database references
Revision 1.3	Sep 27, 2023	Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / citation / database_2 / pdbx_initial_refinement_model / pdbx_struct_oper_list / struct_conn / struct_conn_type Item: _chem_comp.type / _citation.journal_id_CSD ..._chem_comp.type / _citation.journal_id_CSD / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_oper_list.symmetry_operation / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn_type.id

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Structure visualization

Structure viewer	Molecule: MolmilJmol/JSmol

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Downloads & links

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Download

PDBx/mmCIF format	5h9e.cif.gz	1.4 MB	Display	PDBx/mmCIF format
PDB format	pdb5h9e.ent.gz	1.1 MB	Display	PDB format
PDBx/mmJSON format	5h9e.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	5h9e_validation.pdf.gz	563.3 KB	Display	wwPDB validaton report
Full document	5h9e_full_validation.pdf.gz	613 KB	Display
Data in XML	5h9e_validation.xml.gz	109.8 KB	Display
Data in CIF	5h9e_validation.cif.gz	152.8 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/h9/5h9e ftp://data.pdbj.org/pub/pdb/validation_reports/h9/5h9e	HTTPS FTP

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Related structure data

Related structure data	5h9fSC S: Starting model for refinement C: citing same article (ref.)
Similar structure data	5u07-d 4tvx-2 4qyz-d 4u7u-3 5cd4-2 4u7u-2 5u0a-d 3w1h-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#181 - Jan 2015 Cascade and CRISPR similarity (21) #253 - Jan 2021 Expressome similarity (2) #121 - Jan 2010 70S Ribosomes similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (1) #67 - Jul 2005 TATA-Binding Protein similarity (1) #157 - Jan 2013 Transfer-Messenger RNA similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #87 - Mar 2007 Zinc Fingers similarity (2) #91 - Jul 2007 Thymine Dimers similarity (1) #229 - Jan 2019 Fluorescent RNA Aptamers similarity (1) #260 - Aug 2021 Ribonuclease P similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #227 - Nov 2018 Telomerase similarity (2) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (1) #15 - Mar 2001 Transfer RNA similarity (1) #249 - Sep 2020 SARS-CoV-2 RNA-dependent RNA Polymerase similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #135 - Mar 2011 Integrase similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #84 - Dec 2006 Transposase similarity (1) #10 - Oct 2000 Ribosome similarity (1) #210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #105 - Sep 2008 Ribonuclease A similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (1) #245 - May 2020 Spliceosomes similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #65 - May 2005 Self-splicing RNA similarity (1) #98 - Feb 2008 Small Interfering RNA similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #125 - May 2010 Parvoviruses similarity (1) #131 - Nov 2010 Inteins similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #23 - Nov 2001 DNA similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #104 - Aug 2008 Selenocysteine Synthase similarity (1) #106 - Oct 2008 Poly(A) Polymerase similarity (1) #143 - Nov 2011 Toll-like Receptors similarity (1) #161 - May 2013 Ricin similarity (1) #230 - Feb 2019 Initiation Factor eIF4E similarity (1) #266 - Feb 2022 Oligosaccharyltransferase similarity (1) #81 - Sep 2006 Elongation Factors similarity (1) #89 - May 2007 Aconitase and Iron Regulatory Protein 1 similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (2)

PDB pages

PDBj /

wwPDB /

NCBI

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Assembly

Deposited unit

A: CRISPR system Cascade subunit CasA

B: CRISPR system Cascade subunit CasB

C: CRISPR system Cascade subunit CasB

D: CRISPR system Cascade subunit CasC

E: CRISPR system Cascade subunit CasC

F: CRISPR system Cascade subunit CasC

G: CRISPR system Cascade subunit CasC

H: CRISPR system Cascade subunit CasC

I: CRISPR system Cascade subunit CasC

J: CRISPR system Cascade subunit CasD

L: crRNA (61-MER)

M: DNA (47-MER) Non-target

N: DNA (47-MER) Target

K: CRISPR system Cascade subunit CasE

hetero molecules

435 kDa, 14 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	92.807, 149.825, 404.101
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	18
Space group name H-M	P22₁2₁

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Components

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CRISPR system Cascade subunit ... , 5 types, 11 molecules ABCDEFGHIJK

#1: Protein	CRISPR system Cascade subunit CasA / CRISPR type I-E/Ecoli-associated protein CasA/Cse1 / CRISPR-associated protein CasA/Cse1 Mass: 55964.957 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain K12) (bacteria) Strain: K12 / Gene: casA, cse1, ygcL, b2760, JW2730 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46901
#2: Protein	CRISPR system Cascade subunit CasB Mass: 21207.279 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain K12) (bacteria) Strain: K12 / Gene: casB, cse2, ygcK, b2759, JW2729 / Production host: Escherichia coli (E. coli) / References: UniProt: P76632
#3: Protein	CRISPR system Cascade subunit CasC Mass: 40071.402 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain K12) (bacteria) Strain: K12 / Gene: casC, cas4, cse4, ygcJ, b2758, JW2728 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46899
#4: Protein	CRISPR system Cascade subunit CasD Mass: 25239.803 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain K12) (bacteria) Strain: K12 / Gene: casD, cas5, ygcI, b2757, JW5844 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46898
#8: Protein	CRISPR system Cascade subunit CasE / CasE endoRNase / crRNA endonuclease Mass: 22324.002 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (strain K12) (bacteria) Strain: K12 / Gene: casE, cas6e, ygcH, b2756, JW2726 / Production host: Escherichia coli (E. coli) References: UniProt: Q46897, Hydrolases; Acting on ester bonds

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DNA (47-MER) ... , 2 types, 2 molecules MN

#6: DNA chain	DNA (47-MER) Non-target Mass: 14447.288 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)
#7: DNA chain	DNA (47-MER) Target Mass: 14434.274 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Escherichia coli (E. coli)

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RNA chain / Non-polymers , 2 types, 2 molecules L

#5: RNA chain	crRNA (61-MER) Mass: 19677.646 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Production host: Escherichia coli (E. coli)
#9: Chemical	ChemComp-ZN / ZINC ION Mass: 65.409 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Zn

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Experimental details

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Experiment

Experiment	Method: X-RAY DIFFRACTION

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Sample preparation

Crystal	Density Matthews: 3.27 Å³/Da / Density % sol: 62.35 %
Crystal grow	Temperature: 291.95 K / Method: vapor diffusion, hanging drop / pH: 6.2 / Details: 1.6 M Na/K Phosphate

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å
Detector	Type: PSI PILATUS 6M / Detector: PIXEL / Date: Aug 25, 2015
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.979 Å / Relative weight: 1
Reflection	Resolution: 3.2→50.085 Å / Num. obs: 90280 / % possible obs: 96.8 % / Redundancy: 3.6 % / R_merge(I) obs: 0.265 / Net I/σ(I): 5.8

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Processing

Software

Name	Version	Classification
PHENIX	1.8.4_1496	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 5H9F

5h9f

Resolution: 3.21→50.085 Å / SU ML: 0.36 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 23.38 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.247	1997	2.22 %
R_work	0.209	-	-
obs	0.21	90154	97.2 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3.21→50.085 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	24709	2507	1	0	27217

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.005	27955
X-RAY DIFFRACTION	f_angle_d	0.95	38390
X-RAY DIFFRACTION	f_dihedral_angle_d	16.597	10389
X-RAY DIFFRACTION	f_chiral_restr	0.034	4401
X-RAY DIFFRACTION	f_plane_restr	0.004	4569

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
3.2137-3.2941	0.3498	127	0.2821	5612	X-RAY DIFFRACTION	88
3.2941-3.3831	0.3278	141	0.2722	6209	X-RAY DIFFRACTION	97
3.3831-3.4826	0.2902	138	0.2522	6168	X-RAY DIFFRACTION	96
3.4826-3.595	0.2595	144	0.2341	6325	X-RAY DIFFRACTION	99
3.595-3.7235	0.2921	143	0.2235	6351	X-RAY DIFFRACTION	99
3.7235-3.8725	0.286	145	0.2215	6379	X-RAY DIFFRACTION	99
3.8725-4.0487	0.2498	145	0.2099	6377	X-RAY DIFFRACTION	99
4.0487-4.262	0.2198	142	0.1935	6311	X-RAY DIFFRACTION	98
4.262-4.5289	0.2332	143	0.1796	6285	X-RAY DIFFRACTION	97
4.5289-4.8783	0.2057	145	0.1721	6387	X-RAY DIFFRACTION	98
4.8783-5.3687	0.2148	145	0.183	6410	X-RAY DIFFRACTION	99
5.3687-6.1444	0.241	145	0.1986	6392	X-RAY DIFFRACTION	98
6.1444-7.7367	0.2461	145	0.2116	6375	X-RAY DIFFRACTION	96
7.7367-50.0907	0.214	149	0.2003	6576	X-RAY DIFFRACTION	96

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.7544	-1.0264	-0.4302	3.5647	0.9743	1.9788	0.1441	0.7183	-0.1291	-0.8588	-0.2959	-0.313	0.1295	0.334	0.1602	0.8436	0.0954	0.1386	0.6597	0.0348	0.4587	12.5452	1.7508	-134.034
2	1.717	-1.1071	0.2726	3.5891	-0.1516	2.1339	0.1893	0.3588	-0.0734	-0.6928	-0.2355	-0.0666	0.2109	-0.0056	0.0463	0.5924	0.0578	-0.0119	0.4879	-0.0353	0.3949	2.4954	-2.4338	-126.6491
3	6.581	0.1271	-3.2507	1.7441	-0.6002	1.2009	-0.103	0.0501	-0.0895	0.0164	0.0382	0.0092	0.038	-0.0101	0.0511	0.3466	0.0187	-0.0875	0.378	-0.063	0.402	-4.8283	1.5695	-96.0704
4	2.0455	-1.0996	-2.8554	8.613	-0.7696	3.6552	0.6641	0.1043	0.7458	-0.2063	-0.1576	-0.6747	-1.0985	0.8015	-0.4587	1.282	-0.3172	0.1229	0.8545	-0.1015	0.8192	-3.3966	46.3767	-48.2276
5	1.3633	-0.1798	-0.4005	0.0649	-0.3037	3.2972	0.0951	-0.3282	0.3581	0.2459	-0.8136	0.1218	-0.5066	0.0992	0.5179	1.6674	-0.3769	0.0616	0.8076	-0.1732	0.6563	-8.4826	51.3086	-34.9748
6	6.9754	-1.1153	-3.3223	4.8412	-3.7217	5.4541	0.6359	0.1027	0.3188	0.5911	-0.0614	0.4433	-0.258	0.0314	-0.558	1.4367	0.0451	0.0874	0.6416	-0.131	0.5869	-20.273	48.4372	-39.4934
7	4.5781	0.0311	-1.0504	4.7974	0.7686	3.3005	0.4578	0.4039	0.3521	-0.9452	-0.1922	-0.3476	-1.6157	0.3761	-0.2184	1.4736	-0.0931	0.1787	0.6272	0.0084	0.6418	-11.3553	46.8811	-46.2848
8	1.9997	-0.9345	-8.6743	6.0626	-3.1639	8.186	-0.3185	0.6023	0.2868	0.0731	-0.4024	-0.463	-0.6452	1.1519	0.6831	1.1599	-0.1349	0.0166	0.9495	0.1	0.3866	0.4097	30.6646	-49.5713
9	6.038	-2.646	5.4847	4.2403	-3.1427	5.1509	-0.3244	0.6642	0.2162	-0.0769	-0.1814	-0.5119	0.0571	0.3107	0.3671	0.4758	0.0062	-0.1492	0.5659	-0.0298	0.5245	-5.5884	26.1111	-43.1058
10	2.0367	-1.6616	2.7366	6.1435	0.0983	8.4243	-0.5691	-0.2187	0.0248	0.9932	-0.0211	0.5142	0.0087	0.0178	0.6434	0.7556	-0.0859	0.0568	0.6035	-0.1121	0.4367	-16.1088	23.9481	-39.5611
11	2.0042	-1.8337	0.7753	2.0031	2.4673	6.3731	-0.0656	0.5469	0.4284	0.1335	-0.2312	0.7312	-0.0108	-0.1701	0.2749	0.6773	0.0063	0.0077	0.6505	-0.1781	0.5299	-25.3291	33.0667	-45.4434
12	6.525	-3.0234	0.4116	2.4794	-0.8214	2.9038	0.1554	0.3798	0.5541	0.3967	-0.1081	0.3774	-0.6725	0.17	-0.1002	0.8576	-0.163	-0.0554	0.444	-0.0114	0.4725	-15.492	34.1722	-38.3765
13	3.8837	-1.1991	0.9603	3.8661	-1.4175	0.5982	0.5656	-0.3507	0.2648	-0.2288	0.2133	-0.4852	-0.6092	1.5848	-0.5513	0.705	-0.1501	-0.0517	0.7089	-0.1248	0.4551	-2.9093	33.0843	-42.0083
14	2.0254	4.6453	1.6943	6.4832	1.0504	2.2851	0.3519	-0.0133	-0.0938	-1.2311	0.1271	0.2622	0.1806	-0.0557	-0.3633	0.9841	-0.1537	0.1583	0.6208	-0.015	0.7556	-11.1899	36.4271	-52.1892
15	5.4129	-5.4494	2.2919	5.6364	-1.8452	9.7783	-0.0391	-0.0785	0.5823	-0.2213	0.0998	-0.6294	-0.4655	0.2983	0.0695	0.5461	-0.1242	-0.0949	0.4679	-0.0225	0.5335	-10.6913	22.3683	-49.3761
16	7.5144	-1.881	-3.5906	5.0025	-0.5724	8.1487	0.3277	-0.4961	-0.3617	0.2676	-0.0779	-0.2186	0.0869	1.2237	-0.1763	0.4307	0.0396	-0.1124	0.5587	-0.0506	0.3772	-13.9217	3.5323	-54.33
17	7.6971	1.065	2.7232	8.3973	7.954	1.9823	-0.345	-0.4115	1.0252	-0.8274	-0.5678	-0.0717	-0.2336	-0.581	0.7919	0.5723	0.0341	0.0767	0.4588	-0.0089	0.4848	-21.4334	16.4209	-57.3139
18	5.0604	0.142	-0.6975	4.1441	0.2458	5.7191	0.2518	-0.0015	0.3026	-0.0506	-0.2315	-0.3822	-0.1396	0.9453	-0.0368	0.3234	0.0113	0.0083	0.4728	-0.036	0.3549	-11.4998	12.136	-58.0859
19	7.3151	-3.2086	-3.1079	2.0463	5.0442	4.1021	-0.5248	-0.0377	-0.78	0.3319	0.1508	0.2796	0.81	0.7845	0.3231	0.7672	0.1443	-0.0584	0.6045	0.0911	0.4897	-15.384	-5.9998	-64.5981
20	2.8795	1.047	-1.6871	5.8874	-1.728	6.3242	-0.39	0.2322	-0.1691	-0.2588	-0.2542	-0.1303	-0.0944	0.2938	0.5871	0.3737	-0.0166	-0.1671	0.3662	0.0555	0.5895	-24.5066	-4.5278	-73.2105
21	2.8391	-2.4152	-2.7242	6.9602	-0.5976	4.3589	0.2371	0.3589	0.4012	-0.3121	0.4827	0.1342	-0.7526	-0.4927	-0.5641	0.4185	0.0277	-0.0648	0.5338	0.065	0.5031	-25.665	9.0007	-77.1576
22	3.3241	-2.9787	-0.6286	6.5661	0.5452	2.7301	0.3222	-0.1881	0.0599	-0.7387	-0.116	0.0017	0.2348	0.2881	-0.1438	0.3768	0.0056	0.0178	0.4469	0.0032	0.263	-23.9203	3.2674	-67.2229
23	9.7218	-1.6398	1.0489	7.4004	-5.3034	2.0191	0.0075	0.3457	0.3246	-0.3951	0.5783	-0.8596	-0.0731	1.0162	-0.4444	0.4256	-0.052	-0.046	0.4788	-0.0925	0.4991	-11.2482	4.9288	-69.706
24	2.0581	7.0725	-0.2673	2.033	-0.0643	7.9377	-0.0151	0.2187	-0.2572	0.5067	0.0699	-0.7378	-0.7664	0.6382	0.0212	0.394	-0.0022	-0.0518	0.3733	0.0316	0.506	-18.5118	1.1961	-80.4248
25	0.6881	-0.7177	-0.1452	1.4531	-0.1714	0.7113	-0.1885	-0.0296	-0.071	-0.0177	0.1452	-0.1884	-0.7928	0.1406	0.0666	1.6011	-0.0876	0.0668	0.6017	-0.0925	0.5621	-24.0594	58.1146	-12.6979
26	1.2142	-0.7817	0.0486	2.2152	0.5604	1.6046	-0.2144	-0.1096	0.3663	-0.0317	0.3467	-0.6178	-0.5403	0.8503	-0.052	1.2825	-0.3923	-0.0987	1.0144	-0.1325	0.9372	4.9845	50.5871	-9.7689
27	1.8085	-0.2332	0.3148	4.1992	0.8596	2.8028	-0.0145	-0.6173	0.3135	1.0202	0.2343	-0.9429	-0.1353	0.8683	-0.288	1.351	-0.1562	-0.2446	1.2348	-0.166	0.8922	6.367	37.0897	-2.1413
28	1.7688	-0.5556	0.6077	2.0327	0.4076	1.4861	-0.1974	-0.0768	0.1114	0.481	0.1501	-0.057	-0.5422	0.1324	0.0785	1.4355	-0.1271	0.0661	0.6308	-0.0625	0.5061	-22.0201	51.3	-4.4285
29	0.6793	-0.2879	-0.1027	3.2917	1.3595	0.6236	0.1887	-0.7354	-0.0923	0.9515	0.2543	0.5411	-0.8262	-0.6516	-0.2691	1.8375	0.1469	0.3076	0.6815	-0.0682	0.6051	-38.6974	51.7242	1.4139
30	5.4554	1.2338	1.5243	2.0151	4.0086	8.1673	0.222	-0.068	-0.0123	-0.1899	0.2511	0.0298	0.1597	-0.022	-0.2048	2.0972	0.1929	0.3736	0.6763	-0.3317	0.4324	-34.2712	56.4696	10.8632
31	0.6828	-1.2865	-0.253	2.7838	-0.2054	1.2771	0.0921	-0.2532	0.309	0.0738	0.4612	-0.6032	-0.3825	0.6551	-0.4921	2.488	-0.2507	-0.1897	0.269	-0.6191	0.2248	-23.4767	53.7882	11.9488
32	1.9315	0.2783	0.766	1.6042	0.6227	1.1425	-0.1342	-0.247	0.3994	0.2128	-0.0138	0.0461	-0.1938	-0.0719	0.151	0.9776	0.0348	0.1314	0.4466	-0.0564	0.4613	-31.3125	34.4101	-18.0955
33	2.8558	-0.0717	-0.9321	2.7185	-1.354	2.6973	-0.1502	-0.2367	-0.2925	0.3991	0.1134	-0.0936	0.4035	0.2952	0.0284	0.8613	-0.0074	-0.0117	0.4941	-0.0227	0.4002	-17.1646	7.4013	-11.4169
34	1.2475	-0.4201	-0.083	0.6933	0.0736	1.4592	0.0433	-0.1994	0.1633	0.3906	0.0649	0.1344	-0.577	0.0003	-0.0757	1.039	-0.0758	0.0768	0.5272	-0.0692	0.3782	-29.5239	34.079	-15.2625
35	1.5615	-0.1543	0.384	0.4418	-0.559	1.5175	0.0569	-0.2638	0.1248	1.0075	0.1166	0.5784	-0.5247	-0.7849	-0.1171	1.1305	0.0549	0.3134	0.6306	-0.0511	0.67	-49.678	33.8088	-14.3777
36	0.8358	-0.137	0.0163	2.969	-0.2652	1.8606	0.0156	-0.2403	-0.2286	0.6913	0.0107	0.2925	0.1363	-0.1541	-0.0151	0.5362	-0.0182	0.0907	0.4742	0.0222	0.4789	-42.0484	5.9928	-36.871
37	2.079	-0.0266	0.847	1.4637	0.3153	1.7214	0.043	-0.1698	-0.2835	0.148	-0.0852	0.4425	0.1294	-0.3417	0.0627	0.5561	-0.016	0.1927	0.4433	0.0342	0.3936	-46.7845	7.6	-38.844
38	2.6237	0.764	1.5501	1.1355	0.8063	2.1073	0.113	0.0471	0.1219	0.2832	0.0364	0.3211	-0.415	-0.1781	-0.1251	0.7997	0.0029	0.2168	0.4988	-0.0273	0.636	-47.8061	23.9793	-32.1343
39	4.739	2.1366	2.2577	2.3846	0.2787	3.1254	-0.201	-0.0211	0.6621	0.3923	-0.0015	0.6829	-0.4736	-0.564	0.2198	0.6046	0.1672	0.134	0.5322	0.0152	0.5783	-53.5986	35.0393	-45.1812
40	2.1718	-0.1388	0.9514	2.1252	0.4691	3.089	-0.123	-0.5609	0.7272	0.564	-0.3055	1.0561	-0.7575	-1.0021	0.2639	0.7505	0.1176	0.1991	0.8311	-0.0826	0.828	-64.8384	27.6207	-36.8905
41	1.0606	0.1829	0.5454	1.3801	0.3	0.9348	0.0459	-0.0205	0.0079	0.1303	0.0129	0.4033	0.1287	-0.0876	-0.0564	0.3039	0.0604	-0.0161	0.4574	0.045	0.464	-49.8448	13.5364	-66.4426
42	2.2349	0.5279	0.0752	2.7401	0.0871	3.9993	0.203	-0.2034	-0.2829	-0.1977	-0.0504	0.2933	0.6549	-0.1705	-0.1884	0.3206	-0.0275	-0.1174	0.4505	-0.0258	0.7524	-59.9711	-6.8057	-83.7386
43	1.1996	-0.0356	1.0747	0.8287	-0.0349	1.9831	0.0115	-0.0271	0.1007	0.0969	-0.0176	0.268	-0.1572	-0.2467	0.0441	0.3224	0.0312	0.0523	0.3987	0.0168	0.459	-50.009	19.5848	-66.4916
44	4.4071	-1.0421	4.3083	2.5986	-5.2426	2.0178	-0.2549	0.0618	0.4522	0.346	-0.1145	0.8391	-1.6017	-0.0861	0.276	0.5794	0.0611	-0.0648	0.3647	-0.0329	0.7043	-48.3631	41.2973	-69.3843
45	3.3937	1.2424	-1.5097	1.732	0.4096	2.1529	-0.2299	-0.0952	0.5226	-0.1055	-0.0143	1.1447	-0.3758	-0.3465	0.1914	0.5354	0.1102	0.0386	0.5842	0.0266	0.8005	-62.7731	35.5337	-69.6488
46	1.5797	0.6765	-0.2461	1.9689	-0.682	1.261	0.0359	0.0475	0.0452	-0.2153	0.0089	0.2458	0.1647	-0.0719	0.0142	0.2779	0.0441	-0.086	0.3493	-0.0065	0.3874	-36.0017	27.048	-88.2234
47	1.8861	-2.4487	-0.698	6.4456	0.734	1.9447	-0.508	0.445	-0.4671	-0.1531	0.56	2.1643	-0.0072	-0.7919	-0.0474	0.6285	-0.1193	-0.2221	0.7213	0.0881	0.7203	-52.2589	15.1577	-109.7702
48	2.0526	-0.9772	0.4387	2.8517	-2.1714	2.863	0.0071	0.3384	-0.3752	-1.138	0.4461	0.7419	0.5138	-0.563	-0.408	0.6491	-0.0667	-0.2657	0.6414	0.0285	0.7188	-45.0256	18.8419	-117.853
49	1.8304	1.2706	3.3631	2.4156	2.7231	6.3529	-0.0694	0.3549	-0.2833	-0.5522	0.1874	-0.1552	-0.0707	0.504	-0.1019	0.3822	-0.0425	-0.1574	0.495	0.0821	0.5873	-34.2137	27.0166	-103.8354
50	1.1641	1.0181	-0.4898	1.3461	-0.7346	1.6053	-0.0529	0.0519	0.0849	0.1465	0.0101	0.0919	-0.3181	-0.0086	0.0193	0.2587	0.1009	-0.0612	0.3514	-0.0682	0.4968	-39.4837	25.8831	-86.4105
51	1.7777	1.4436	-1.2344	6.9237	-3.4469	5.814	-0.0706	-0.0534	0.3041	-0.0228	0.2224	0.6241	-0.1788	-0.2161	-0.113	0.2398	0.0866	-0.0298	0.4161	-0.0064	0.4356	-41.556	31.5725	-84.0098
52	2.0759	0.4731	-0.3786	1.1254	-3.017	8.5469	-0.1086	0.1545	0.0684	-0.034	0.095	0.1192	-0.2842	-0.2576	0.0585	0.3388	-0.0795	0.0533	0.3021	-0.0345	0.5383	-25.7865	38.8981	-83.4796
53	2.0031	7.5916	1.883	2.0555	-9.2069	1.9609	0.68	-0.2487	0.683	0.5534	-0.236	0.1904	-0.7642	0.5025	-0.4275	0.6388	0.1292	0.0592	0.4137	-0.0015	0.501	-26.7931	45.7112	-82.9998
54	1.7661	-1.732	-0.5996	1.6264	0.6951	2.6058	-0.4275	-0.5661	0.6926	0.1307	0.205	0.2059	-0.717	-0.1623	0.0241	0.3979	0.046	-0.0104	0.4732	-0.005	0.5788	-39.8304	48.5675	-90.9121
55	1.046	-1.3463	0.8752	1.6463	-0.9433	3.5623	0.2718	0.1262	0.8245	0.1739	-0.3423	-0.2161	0.1296	0.358	0.1429	0.4311	0.0085	-0.0962	0.3063	-0.1077	0.5498	-3.312	42.8547	-99.855
56	1.5472	-0.378	-0.678	0.3544	-0.2422	1.0473	-0.1657	0.1518	0.5094	-0.0297	0.098	-0.4683	0.1078	0.0373	-0.0348	0.226	-0.0522	-0.0139	0.6269	0.0667	0.3011	-19.7576	26.8946	-96.9099
57	2.0126	-8.4499	-8.5309	2.005	9.4349	2.0066	0.0189	0.1814	-0.142	0.3236	0.281	-0.3484	0.0634	0.077	-0.121	0.3648	-0.0794	-0.119	0.4841	0.0355	0.4401	-18.0431	16.4607	-100.6772
58	0.8131	-1.2475	-0.1814	5.9392	-5.4652	8.1522	0.1217	-0.3146	-0.2234	0.4069	-0.1685	0.8249	0.429	-0.7705	0.1334	0.225	0.0372	-0.1203	0.3069	-0.0527	0.4669	-7.2327	22.4924	-95.3751
59	0.8693	1.077	-1.5359	2.237	-0.9092	4.5197	-0.2415	0.309	0.1956	-0.4018	0.043	0.1186	-0.0587	-0.3059	0.1784	0.238	-0.0318	-0.0091	0.3689	0.0564	0.4435	-13.6807	33.7025	-104.3897
60	6.1557	0.7769	-0.8352	9.6222	-2.1524	2.0453	0.0062	0.9707	0.8607	-1.4926	0.1337	0.7325	-0.6714	-0.9085	-0.1791	0.8347	-0.0261	-0.0431	0.6521	0.177	0.7189	-11.3694	50.2677	-124.5237
61	5.633	-1.6922	-2.0923	9.6787	2.038	8.9388	0.0634	0.0464	0.1319	-0.662	0.0202	-0.9507	-0.2871	1.1364	-0.2286	0.5882	-0.0276	0.0389	0.769	0.2121	1.0773	5.0634	46.5907	-136.5598
62	1.6903	-0.8105	1.8061	1.6289	0.4583	3.3124	0.3014	0.0632	0.2645	-0.162	0.3066	0.2313	-0.0706	-0.2736	-0.5115	1.1968	0.0431	0.2813	0.7099	0.2484	0.9476	-4.2976	45.4755	-141.4675
63	1.4899	0.0563	-1.0439	1.6403	-0.3445	5.4341	0.0012	0.5185	0.1945	-0.666	-0.083	0.0781	-0.3864	-0.1559	0.1205	0.567	0.0305	-0.0966	0.4939	0.0965	0.5425	-6.709	36.4681	-125.7939
64	2.3748	0.8057	-0.462	1.4157	-0.2126	0.8448	0.0922	-0.1505	0.2471	0.0137	-0.009	0.0424	-0.1618	0.0604	-0.073	0.3382	0.0461	-0.0747	0.322	-0.0408	0.3766	-13.0348	34.9377	-96.9591
65	9.0886	-4.2622	0.8041	5.8636	0.3554	3.1807	-0.1388	-0.5665	0.9033	-0.1335	0.049	-0.235	-0.1549	0.2585	0.1398	0.3814	-0.0432	-0.0468	0.4092	-0.0605	0.5975	-4.9208	50.481	-92.71
66	2.0049	-5.707	-3.0488	4.5041	2.257	7.4833	0.0665	-0.3128	0.3458	0.3407	0.0421	-1.9173	0.1873	1.6058	-0.1104	0.4009	-0.1349	0.0127	0.762	0.0687	0.9761	28.3766	30.6807	-103.3291
67	1.3425	2.6461	-1.8286	6.7142	-0.9752	7.0552	-0.1248	-0.3057	0.3096	-0.0399	-0.3093	0.0414	-0.1107	0.1168	0.348	0.2521	0.0131	-0.0465	0.3893	-0.0346	0.4719	13.4607	30.4132	-99.2072
68	1.6108	1.6257	0.8298	6.2166	2.3797	3.3389	-0.0129	-0.2038	0.141	0.0445	-0.3216	-0.1024	0.0707	0.1861	0.4677	0.3885	-0.0245	-0.0247	0.3156	0.038	0.578	3.199	21.483	-106.4253
69	7.5337	0.8816	-2.7221	8.8391	0.2616	7.2439	-0.4752	0.0654	0.3664	-0.976	-0.1401	0.2032	0.1471	-0.1241	0.5334	0.6586	0.0331	-0.093	0.3294	0.0035	0.3583	-2.7207	16.0508	-116.5197
70	4.9162	1.923	4.6039	1.6967	1.73	4.3261	-0.2589	0.1666	0.1801	-0.4476	-0.1509	-0.0052	-0.1039	-0.257	0.396	0.3263	0.1464	-0.0344	0.2873	0.0184	0.6455	6.5625	17.1318	-104.6036
71	2.0004	-7.6378	-9.3698	2.0005	1.9997	1.9999	0.175	0.6557	-0.9201	-0.4614	-0.1239	0.3894	0.7465	0.2954	-0.0652	0.0691	0.1459	0.0675	0.1302	-0.1631	0.6022	10.7311	21.9562	-107.5588
72	2.4007	-3.1815	0.0195	4.1863	0.0355	2.0162	0.1391	0.3945	0.5957	-0.394	0.0905	0.4263	-0.1314	0.511	-0.2687	0.2369	-0.0014	-0.0223	0.4126	0.0752	0.5135	12.2089	29.846	-111.646
73	2.0061	3.9452	4.5449	6.6997	2.2268	2.0625	0.0182	1.0113	0.6255	-0.768	-0.6452	0.2462	-0.6624	0.409	0.4946	0.6183	0.0258	-0.0607	0.814	0.1317	0.5293	9.1708	36.3302	-120.5048
74	6.9201	-3.2528	5.884	2.0079	-7.8151	2.0074	-0.0024	0.7231	0.4942	-0.7531	-0.5933	-1.3128	0.3536	0.7244	0.5426	0.5701	0.0744	0.0119	0.664	0.0868	0.5358	18.103	31.3035	-120.6428
75	1.8013	-0.3358	-0.5319	2.3029	-0.5386	4.3133	-0.0205	-0.182	0.0215	-0.0789	0.097	0.4648	0.3073	0.2722	-0.1147	0.2744	0.007	-0.0053	0.526	0.07	0.5995	14.2171	23.3622	-97.9993
76	9.4741	1.6416	4.5334	9.5058	2.5891	2.0091	0.2003	-2.1181	0.7586	2.2881	0.4332	0.6613	1.1199	-0.7185	-0.5175	0.799	-0.0221	0.0932	0.6234	-0.1746	0.4986	5.4044	19.2778	-85.5287
77	2.0529	-8.3691	7.7231	8.4904	-7.7742	7.6217	-0.5669	-0.7641	0.3655	-0.1902	0.0975	0.516	-0.1467	-0.1544	0.3747	0.3014	-0.0586	-0.0954	0.3206	0.0501	0.5906	-9.9025	20.3065	-107.1054
78	6.7815	0.8538	0.2686	3.5943	-2.3608	2.3319	-0.0327	1.5504	-0.1179	-0.9925	-0.205	0.3928	1.0294	-0.4815	0.1812	0.89	0.0629	-0.2732	0.7552	-0.1105	0.4335	-13.6569	25.3788	-126.5766
79	2.0463	-0.2278	1.7542	1.3271	0.2782	1.4914	0.2189	0.4257	-0.058	-0.1751	-0.1009	-0.469	-0.2065	0.2379	-0.1462	0.3221	0.0785	0.0319	0.4612	0.083	0.474	8.9969	25.0389	-108.0778
80	3.8751	-2.4024	2.9233	5.9351	0.8146	6.7788	0.1089	0.5346	0.8118	0.049	-0.1913	-0.6468	-0.4922	0.7976	0.084	0.4854	-0.131	-0.0388	0.4749	0.0678	0.7713	14.6283	40.6435	-108.4223
81	1.8072	0.7783	0.4896	2.1278	-0.0316	2.7883	0.0675	0.2798	0.2333	0.0676	0.0366	-0.1815	0.1218	0.3218	-0.0973	0.3367	0.0086	-0.0816	0.4715	0.0405	0.5518	9.2497	32.8173	-102.1976
82	3.5611	2.8188	-0.5849	5.0016	1.3699	5.5434	0.3252	-0.7482	0.38	0.1232	-0.0679	-0.5858	0.0369	1.062	-0.3556	0.3766	-0.0766	-0.1588	0.6664	-0.0097	0.6536	26.8698	24.8009	-97.2151
83	1.0994	0.7708	0.0779	3.1863	-0.4346	2.8608	-0.4557	-0.1829	0.1967	0.074	0.0865	-0.554	0.0416	0.7072	0.28	0.3841	0.1808	-0.1181	0.7036	0.0709	0.6933	20.7273	20.9342	-98.9161
84	6.6681	-2.6631	3.9102	5.2169	-2.0323	7.8804	0.0787	0.1109	0.1295	-0.1273	-0.1641	-0.955	0.1009	0.4466	0.094	0.4047	-0.0648	-0.0543	0.484	0.0546	0.6057	18.7685	14.3292	-110.3485
85	0.3098	0.1365	0.2033	0.0786	0.0889	0.1271	0.1426	0.5334	0.6531	-0.4616	-0.2699	-0.4278	-0.8902	0.43	0.1712	0.5258	0.1048	0.0288	0.6432	0.6463	0.3125	9.2929	24.3316	-124.8036
86	7.2428	-1.4583	6.9365	3.3415	-1.2359	2.0297	0.3418	0.4426	0.527	-0.0434	-0.3676	-0.6894	0.5096	0.0086	-0.1072	0.3398	0.0061	0.0721	0.5168	-0.0171	0.5813	17.6075	15.6409	-109.2967
87	3.5149	-1.6298	-1.0873	1.9998	1.3927	1.9629	0.0235	0.2805	0.111	-0.0861	0.1579	-0.0134	0.0595	0.4495	-0.1033	0.3647	-0.0057	-0.0731	0.449	0.1516	0.6196	2.6499	27.6816	-115.279
88	8.19	6.989	-5.3871	7.6439	-4.6725	3.5554	0.1541	1.0767	-0.0124	-0.1197	-0.0314	0.0657	-0.1358	-0.0299	-0.0368	0.375	0.0122	-0.163	0.4796	0.118	0.3476	-14.9723	26.1313	-111.1802
89	0.0135	0.0738	-0.0954	0.8907	-0.2371	0.3398	0.0663	-0.0407	0.0473	0.3073	-0.0043	0.1162	0.0625	0.071	-0.0305	0.3756	0.0052	0.0285	0.4057	-0.0327	0.3867	-34.1737	16.9669	-56.3687
90	0.9516	-0.127	-0.1097	0.2185	-0.8013	3.0963	-0.0353	0.0287	0.73	-0.4815	-0.0654	-0.669	-1.0907	0.2081	-0.0568	1.735	-0.3381	0.2797	0.6784	-0.2598	0.7877	-12.3197	55.8819	-17.5539
91	1.2383	1.4438	0.872	1.6544	1.0124	0.6208	0.2876	-0.0824	0.0983	-0.3394	0.1751	0.6509	-0.2632	0.0618	-0.321	2.6904	-0.9919	0.7047	0.7389	-0.4825	1.6008	-7.5895	73.3079	-22.9644
92	0.0247	0.0146	0.0167	0.0143	0.0178	0.0166	-0.9967	0.0213	-0.2383	-0.6848	1.1064	-0.1499	-0.9387	0.4502	-0.039	3.6603	-0.6839	0.6917	1.3308	-0.1501	1.4594	-11.1746	93.1066	-33.9445
93	2.2574	-1.6902	-0.018	1.4406	-0.5696	1.7409	0.0216	-0.5209	0.9322	-0.291	0.0133	-1.3735	-0.4178	0.4464	-0.2745	3.5966	-0.5039	0.4092	0.9384	-0.2865	1.506	-14.2421	93.5604	-28.7596
94	5.6449	2.767	0.6749	6.9545	-3.3269	8.908	0.9789	1.5335	1.9348	-0.753	-0.3538	2.0489	-1.7727	-0.5825	-0.6303	1.4489	0.3174	-0.0621	1.0742	0.1529	1.3659	-24.3865	11.3822	-133.6922
95	2.5444	-0.164	-2.2633	3.8159	-5.6733	2.022	0.3233	0.7994	0.5864	-1.4482	0.6919	-0.035	-0.9451	-0.3214	-0.9123	1.3901	-0.1135	-0.0569	0.8289	0.1031	1.0112	-13.2644	-6.7283	-102.3786
96	2.0007	3.3914	-1.3195	1.313	-1.0624	1.6366	0.3031	0.3593	-0.1658	-0.2329	-0.0507	-0.0128	0.2655	-0.1848	-0.1962	2.2518	0.1518	-0.1193	1.9534	-0.2626	1.3346	-5.5802	2.8059	-79.0614
97	7.8502	-5.3563	1.6509	7.1005	2.6801	5.8233	-0.2106	0.2499	1.0114	0.1572	-0.6894	-0.2705	-1.1649	0.1734	0.785	1.8287	-0.5867	0.0958	0.8416	-0.1619	0.579	-5.8239	53.3766	-26.6373
98	0.3667	0.2451	0.1363	0.3685	-0.3964	0.5637	0.0787	-0.0299	0.117	0.1489	-0.0226	0.0476	0.1119	0.0025	-0.0228	0.4073	0.1068	0.001	0.3644	-0.0533	0.4113	-26.5429	9.7537	-68.176
99	0.0424	0.1704	-0.101	1.0045	-0.6338	0.372	-0.1537	2.8239	1.5859	-2.0964	-0.8467	2.2391	-1.1259	-3.578	0.8098	1.7918	0.6937	-0.6434	2.0616	0.0992	1.2052	-23.4788	12.4238	-139.9653
100	1.2017	-0.5503	0.7789	0.2609	-0.3783	0.5004	0.2997	0.8638	0.154	-0.5789	0.305	-0.1529	0.2394	-0.0763	-0.5745	2.5102	-0.0577	-0.1342	1.1983	-0.0983	0.9077	-31.9278	76.0071	-45.0168
101	1.3636	0.5115	-1.8946	2.7133	-0.9704	2.6509	-0.3005	0.7464	0.3151	-0.5902	-0.4393	0.0473	-0.4211	0.211	0.6294	2.4125	-0.4424	0.4857	1.2247	-0.159	0.917	-25.9727	80.606	-47.864
102	2.0834	1.3424	-0.298	1.9851	0.7779	5.4905	0.2191	0.4262	-0.4767	-1.3028	0.4921	1.3485	0.7198	0.3302	-0.5988	2.8808	0.1338	-0.0072	0.7817	0.0642	1.1941	-34.2246	78.8447	-42.6229
103	1.0563	-0.4182	-0.1157	2.1444	2.1339	3.7085	-0.4905	0.1332	-0.1848	-1.7785	-0.324	1.4103	-1.3934	-0.2148	0.3337	2.8423	-0.1061	0.0825	0.5974	0.1402	0.0041	-30.5102	80.1542	-27.3279
104	1.5551	-2.151	0.8547	7.3051	0.4533	1.0004	-0.2426	0.0972	0.0225	-0.1144	0.0544	0.2036	-0.4637	-0.2109	0.0892	2.8674	0.0494	-0.1577	0.684	-0.2321	-0.2579	-28.6789	80.0645	-18.9066
105	0.3231	0.4038	0.4149	0.9544	-0.2381	0.9767	-0.3214	0.0381	0.8481	-1.1499	0.3979	-0.628	-1.2175	0.7778	-0.1997	3.1661	-0.0616	0.0103	0.4006	-0.5479	0.2778	-27.184	80.5054	-28.25
106	7.2697	-2.4058	-2.1363	1.1882	1.5769	2.7693	-0.4512	-0.2028	-1.432	-1.0776	0.0194	0.1368	-0.41	0.5546	0.4039	2.6374	-0.0635	-0.183	0.7306	-0.1028	0.7459	-30.6483	80.0001	-30.3816

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	CHAIN 'A' AND (RESID 2 THROUGH 130 )
2	X-RAY DIFFRACTION	2	CHAIN 'A' AND (RESID 131 THROUGH 375 )
3	X-RAY DIFFRACTION	3	CHAIN 'A' AND (RESID 376 THROUGH 495 )
4	X-RAY DIFFRACTION	4	CHAIN 'B' AND (RESID 3 THROUGH 16 )
5	X-RAY DIFFRACTION	5	CHAIN 'B' AND (RESID 17 THROUGH 26 )
6	X-RAY DIFFRACTION	6	CHAIN 'B' AND (RESID 27 THROUGH 37 )
7	X-RAY DIFFRACTION	7	CHAIN 'B' AND (RESID 38 THROUGH 75 )
8	X-RAY DIFFRACTION	8	CHAIN 'B' AND (RESID 76 THROUGH 86 )
9	X-RAY DIFFRACTION	9	CHAIN 'B' AND (RESID 87 THROUGH 94 )
10	X-RAY DIFFRACTION	10	CHAIN 'B' AND (RESID 95 THROUGH 106 )
11	X-RAY DIFFRACTION	11	CHAIN 'B' AND (RESID 107 THROUGH 111 )
12	X-RAY DIFFRACTION	12	CHAIN 'B' AND (RESID 112 THROUGH 124 )
13	X-RAY DIFFRACTION	13	CHAIN 'B' AND (RESID 125 THROUGH 129 )
14	X-RAY DIFFRACTION	14	CHAIN 'B' AND (RESID 130 THROUGH 142 )
15	X-RAY DIFFRACTION	15	CHAIN 'B' AND (RESID 143 THROUGH 159 )
16	X-RAY DIFFRACTION	16	CHAIN 'C' AND (RESID 3 THROUGH 26 )
17	X-RAY DIFFRACTION	17	CHAIN 'C' AND (RESID 27 THROUGH 37 )
18	X-RAY DIFFRACTION	18	CHAIN 'C' AND (RESID 38 THROUGH 67 )
19	X-RAY DIFFRACTION	19	CHAIN 'C' AND (RESID 68 THROUGH 86 )
20	X-RAY DIFFRACTION	20	CHAIN 'C' AND (RESID 87 THROUGH 98 )
21	X-RAY DIFFRACTION	21	CHAIN 'C' AND (RESID 99 THROUGH 111 )
22	X-RAY DIFFRACTION	22	CHAIN 'C' AND (RESID 112 THROUGH 129 )
23	X-RAY DIFFRACTION	23	CHAIN 'C' AND (RESID 130 THROUGH 141 )
24	X-RAY DIFFRACTION	24	CHAIN 'C' AND (RESID 142 THROUGH 156 )
25	X-RAY DIFFRACTION	25	CHAIN 'D' AND (RESID 2 THROUGH 50 )
26	X-RAY DIFFRACTION	26	CHAIN 'D' AND (RESID 51 THROUGH 115 )
27	X-RAY DIFFRACTION	27	CHAIN 'D' AND (RESID 116 THROUGH 155 )
28	X-RAY DIFFRACTION	28	CHAIN 'D' AND (RESID 156 THROUGH 304 )
29	X-RAY DIFFRACTION	29	CHAIN 'D' AND (RESID 305 THROUGH 334 )
30	X-RAY DIFFRACTION	30	CHAIN 'D' AND (RESID 335 THROUGH 349 )
31	X-RAY DIFFRACTION	31	CHAIN 'D' AND (RESID 350 THROUGH 361 )
32	X-RAY DIFFRACTION	32	CHAIN 'E' AND (RESID 2 THROUGH 57 )
33	X-RAY DIFFRACTION	33	CHAIN 'E' AND (RESID 58 THROUGH 170 )
34	X-RAY DIFFRACTION	34	CHAIN 'E' AND (RESID 171 THROUGH 279 )
35	X-RAY DIFFRACTION	35	CHAIN 'E' AND (RESID 280 THROUGH 361 )
36	X-RAY DIFFRACTION	36	CHAIN 'F' AND (RESID 2 THROUGH 115 )
37	X-RAY DIFFRACTION	37	CHAIN 'F' AND (RESID 116 THROUGH 260 )
38	X-RAY DIFFRACTION	38	CHAIN 'F' AND (RESID 261 THROUGH 285 )
39	X-RAY DIFFRACTION	39	CHAIN 'F' AND (RESID 286 THROUGH 322 )
40	X-RAY DIFFRACTION	40	CHAIN 'F' AND (RESID 323 THROUGH 362 )
41	X-RAY DIFFRACTION	41	CHAIN 'G' AND (RESID 2 THROUGH 79 )
42	X-RAY DIFFRACTION	42	CHAIN 'G' AND (RESID 80 THROUGH 146 )
43	X-RAY DIFFRACTION	43	CHAIN 'G' AND (RESID 147 THROUGH 304 )
44	X-RAY DIFFRACTION	44	CHAIN 'G' AND (RESID 305 THROUGH 322 )
45	X-RAY DIFFRACTION	45	CHAIN 'G' AND (RESID 323 THROUGH 362 )
46	X-RAY DIFFRACTION	46	CHAIN 'H' AND (RESID 2 THROUGH 57 )
47	X-RAY DIFFRACTION	47	CHAIN 'H' AND (RESID 58 THROUGH 84 )
48	X-RAY DIFFRACTION	48	CHAIN 'H' AND (RESID 85 THROUGH 170 )
49	X-RAY DIFFRACTION	49	CHAIN 'H' AND (RESID 171 THROUGH 190 )
50	X-RAY DIFFRACTION	50	CHAIN 'H' AND (RESID 191 THROUGH 260 )
51	X-RAY DIFFRACTION	51	CHAIN 'H' AND (RESID 261 THROUGH 284 )
52	X-RAY DIFFRACTION	52	CHAIN 'H' AND (RESID 285 THROUGH 304 )
53	X-RAY DIFFRACTION	53	CHAIN 'H' AND (RESID 305 THROUGH 322 )
54	X-RAY DIFFRACTION	54	CHAIN 'H' AND (RESID 323 THROUGH 361 )
55	X-RAY DIFFRACTION	55	CHAIN 'I' AND (RESID 2 THROUGH 11 )
56	X-RAY DIFFRACTION	56	CHAIN 'I' AND (RESID 12 THROUGH 21 )
57	X-RAY DIFFRACTION	57	CHAIN 'I' AND (RESID 22 THROUGH 26 )
58	X-RAY DIFFRACTION	58	CHAIN 'I' AND (RESID 27 THROUGH 31 )
59	X-RAY DIFFRACTION	59	CHAIN 'I' AND (RESID 32 THROUGH 57 )
60	X-RAY DIFFRACTION	60	CHAIN 'I' AND (RESID 58 THROUGH 71 )
61	X-RAY DIFFRACTION	61	CHAIN 'I' AND (RESID 72 THROUGH 84 )
62	X-RAY DIFFRACTION	62	CHAIN 'I' AND (RESID 85 THROUGH 97 )
63	X-RAY DIFFRACTION	63	CHAIN 'I' AND (RESID 107 THROUGH 195 )
64	X-RAY DIFFRACTION	64	CHAIN 'I' AND (RESID 196 THROUGH 319 )
65	X-RAY DIFFRACTION	65	CHAIN 'I' AND (RESID 320 THROUGH 361 )
66	X-RAY DIFFRACTION	66	CHAIN 'J' AND (RESID 1 THROUGH 5 )
67	X-RAY DIFFRACTION	67	CHAIN 'J' AND (RESID 6 THROUGH 10 )
68	X-RAY DIFFRACTION	68	CHAIN 'J' AND (RESID 11 THROUGH 20 )
69	X-RAY DIFFRACTION	69	CHAIN 'J' AND (RESID 21 THROUGH 25 )
70	X-RAY DIFFRACTION	70	CHAIN 'J' AND (RESID 26 THROUGH 30 )
71	X-RAY DIFFRACTION	71	CHAIN 'J' AND (RESID 31 THROUGH 32 )
72	X-RAY DIFFRACTION	72	CHAIN 'J' AND (RESID 33 THROUGH 43 )
73	X-RAY DIFFRACTION	73	CHAIN 'J' AND (RESID 44 THROUGH 50 )
74	X-RAY DIFFRACTION	74	CHAIN 'J' AND (RESID 51 THROUGH 60 )
75	X-RAY DIFFRACTION	75	CHAIN 'J' AND (RESID 61 THROUGH 72 )
76	X-RAY DIFFRACTION	76	CHAIN 'J' AND (RESID 73 THROUGH 79 )
77	X-RAY DIFFRACTION	77	CHAIN 'J' AND (RESID 80 THROUGH 89 )
78	X-RAY DIFFRACTION	78	CHAIN 'J' AND (RESID 90 THROUGH 102 )
79	X-RAY DIFFRACTION	79	CHAIN 'J' AND (RESID 103 THROUGH 129 )
80	X-RAY DIFFRACTION	80	CHAIN 'J' AND (RESID 130 THROUGH 143 )
81	X-RAY DIFFRACTION	81	CHAIN 'J' AND (RESID 144 THROUGH 163 )
82	X-RAY DIFFRACTION	82	CHAIN 'J' AND (RESID 164 THROUGH 173 )
83	X-RAY DIFFRACTION	83	CHAIN 'J' AND (RESID 174 THROUGH 187 )
84	X-RAY DIFFRACTION	84	CHAIN 'J' AND (RESID 188 THROUGH 201 )
85	X-RAY DIFFRACTION	85	CHAIN 'J' AND (RESID 202 THROUGH 206 )
86	X-RAY DIFFRACTION	86	CHAIN 'J' AND (RESID 207 THROUGH 219 )
87	X-RAY DIFFRACTION	87	CHAIN 'L' AND (RESID 1 THROUGH 5 )
88	X-RAY DIFFRACTION	88	CHAIN 'L' AND (RESID 6 THROUGH 10 )
89	X-RAY DIFFRACTION	89	CHAIN 'L' AND (RESID 11 THROUGH 35 )
90	X-RAY DIFFRACTION	90	CHAIN 'L' AND (RESID 36 THROUGH 40 )
91	X-RAY DIFFRACTION	91	CHAIN 'L' AND (RESID 41 THROUGH 43 )
92	X-RAY DIFFRACTION	92	CHAIN 'L' AND (RESID 44 THROUGH 53 )
93	X-RAY DIFFRACTION	93	CHAIN 'L' AND (RESID 54 THROUGH 60 )
94	X-RAY DIFFRACTION	94	CHAIN 'M' AND (RESID 11 THROUGH 18 )
95	X-RAY DIFFRACTION	95	CHAIN 'M' AND (RESID 19 THROUGH 28 )
96	X-RAY DIFFRACTION	96	CHAIN 'M' AND (RESID 29 THROUGH 29 )
97	X-RAY DIFFRACTION	97	CHAIN 'N' AND (RESID 1 THROUGH 5 )
98	X-RAY DIFFRACTION	98	CHAIN 'N' AND (RESID 6 THROUGH 34 )
99	X-RAY DIFFRACTION	99	CHAIN 'N' AND (RESID 35 THROUGH 40 )
100	X-RAY DIFFRACTION	100	CHAIN 'K' AND (RESID 1 THROUGH 15 )
101	X-RAY DIFFRACTION	101	CHAIN 'K' AND (RESID 16 THROUGH 24 )
102	X-RAY DIFFRACTION	102	CHAIN 'K' AND (RESID 25 THROUGH 63 )
103	X-RAY DIFFRACTION	103	CHAIN 'K' AND (RESID 64 THROUGH 129 )
104	X-RAY DIFFRACTION	104	CHAIN 'K' AND (RESID 130 THROUGH 142 )
105	X-RAY DIFFRACTION	105	CHAIN 'K' AND (RESID 143 THROUGH 181 )
106	X-RAY DIFFRACTION	106	CHAIN 'K' AND (RESID 182 THROUGH 199 )

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-CRISPR system Cascade subunit ... , 5 types, 11 molecules ABCDEFGHIJK

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CRISPR system Cascade subunit ... , 5 types, 11 molecules ABCDEFGHIJK

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DNA (47-MER) ... , 2 types, 2 molecules MN

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RNA chain / Non-polymers , 2 types, 2 molecules L

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Experimental details

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Experiment

-
Sample preparation

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Data collection

-
Processing

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About Yorodumi

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News

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Feb 9, 2022. New format data for meta-information of EMDB entries

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Aug 12, 2020. Covid-19 info

+
Mar 5, 2020. Novel coronavirus structure data

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

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Jul 12, 2017. Major update of PDB

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