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Yorodumi- PDB-5gwm: Solution structure of heterodimeric coiled-coil domain of Drosoph... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5gwm | ||||||
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Title | Solution structure of heterodimeric coiled-coil domain of Drosophila GABAB receptor 1 and 3 | ||||||
Components |
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Keywords | SIGNALING PROTEIN / GABAB receptor / Drosophila / coiled-coil | ||||||
Function / homology | Function and homology information G protein-coupled GABA receptor activity / G protein-coupled receptor heterodimeric complex / sleep / gamma-aminobutyric acid signaling pathway / transmembrane signaling receptor activity / membrane => GO:0016020 / G protein-coupled receptor signaling pathway / membrane Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Liu, X. / Zhang, S. / Zhang, C.X. / Liu, J. | ||||||
Citation | Journal: To Be Published Title: Solution structure of heterodimeric coiled-coil domain of Drosophila GABAB receptor 1 and 3 Authors: Liu, X. / Zhang, S. / Zhang, C.X. / Liu, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5gwm.cif.gz | 669 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5gwm.ent.gz | 562.3 KB | Display | PDB format |
PDBx/mmJSON format | 5gwm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5gwm_validation.pdf.gz | 565.5 KB | Display | wwPDB validaton report |
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Full document | 5gwm_full_validation.pdf.gz | 1.3 MB | Display | |
Data in XML | 5gwm_validation.xml.gz | 134.2 KB | Display | |
Data in CIF | 5gwm_validation.cif.gz | 119.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gw/5gwm ftp://data.pdbj.org/pub/pdb/validation_reports/gw/5gwm | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6327.121 Da / Num. of mol.: 1 / Fragment: coiled-coil domain, UNP residues 751-802 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: GABA-B-R1, CG15274 / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q9BML7 |
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#2: Protein/peptide | Mass: 5954.819 Da / Num. of mol.: 1 / Fragment: coiled-coil domain, UNP residues 914-954 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: GABA-B-R3, CG3022, Dmel_CG3022 / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q9VPS7 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 120 mM / Label: condition1 / pH: 7.0 / Pressure: 1 atm / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Agilent DD2 / Manufacturer: Agilent / Model: DD2 / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: medoid | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |