ジャーナル: Science / 年: 2016 タイトル: Structural basis for the gating mechanism of the type 2 ryanodine receptor RyR2. 著者: Wei Peng / Huaizong Shen / Jianping Wu / Wenting Guo / Xiaojing Pan / Ruiwu Wang / S R Wayne Chen / Nieng Yan / 要旨: RyR2 is a high-conductance intracellular calcium (Ca) channel that controls the release of Ca from the sarco(endo)plasmic reticulum of a variety of cells. Here, we report the structures of RyR2 from ...RyR2 is a high-conductance intracellular calcium (Ca) channel that controls the release of Ca from the sarco(endo)plasmic reticulum of a variety of cells. Here, we report the structures of RyR2 from porcine heart in both the open and closed states at near-atomic resolutions determined using single-particle electron cryomicroscopy. Structural comparison reveals a breathing motion of the overall cytoplasmic region resulted from the interdomain movements of amino-terminal domains (NTDs), Helical domains, and Handle domains, whereas almost no intradomain shifts are observed in these armadillo repeats-containing domains. Outward rotations of the Central domains, which integrate the conformational changes of the cytoplasmic region, lead to the dilation of the cytoplasmic gate through coupled motions. Our structural and mutational characterizations provide important insights into the gating and disease mechanism of RyRs.
履歴
登録
2016年7月26日
登録サイト: PDBJ / 処理サイト: PDBJ
改定 1.0
2016年10月5日
Provider: repository / タイプ: Initial release
改定 1.1
2017年2月8日
Group: Database references
改定 1.2
2019年11月6日
Group: Data collection / Other / カテゴリ: cell / em_image_scans / Item: _cell.Z_PDB