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Open data
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Basic information
Entry | Database: PDB / ID: 5g2f | ||||||
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Title | Type IV-like competence pilin TTHA1222 from Thermus thermophilus | ||||||
![]() | TYPE-IV LIKE PILIN TTHA1222 | ||||||
![]() | UNKNOWN FUNCTION / NATURAL COMPETENCE / TYPE IV PILUS / DNA UPTAKE | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Karuppiah, V. / Derrick, J.P. | ||||||
![]() | ![]() Title: Structures of Type Iv Pilins from Thermus Thermophilus Demonstrate Similarities with Type II Secretion System Pseudopilins Authors: Karuppiah, V. / Thistlethwaite, A. / Derrick, J.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 100.3 KB | Display | ![]() |
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PDB format | ![]() | 84.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 455.4 KB | Display | ![]() |
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Full document | ![]() | 458.2 KB | Display | |
Data in XML | ![]() | 12 KB | Display | |
Data in CIF | ![]() | 16.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.0801, 0.9963, 0.0321), Vector: |
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Components
#1: Protein | Mass: 10195.503 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.57 % / Description: NONE |
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Crystal grow | Details: 0.2M NA/K TARTRATE 0.1M SODIUM CITRATE PH 5.6 2.0 M AMMONIUM SULFATE |
-Data collection
Diffraction | Mean temperature: 77 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PILATUS / Detector: PIXEL / Date: Jul 14, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9512 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→31.86 Å / Num. obs: 28582 / % possible obs: 99 % / Observed criterion σ(I): 3.1 / Redundancy: 6.9 % / Rmerge(I) obs: 0.03 / Net I/σ(I): 34.2 |
Reflection shell | Resolution: 1.85→1.9 Å / Redundancy: 7.1 % / Rmerge(I) obs: 0.68 / Mean I/σ(I) obs: 3.1 / % possible all: 97.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.85→63.72 Å / Cor.coef. Fo:Fc: 0.964 / Cor.coef. Fo:Fc free: 0.949 / SU B: 5.124 / SU ML: 0.075 / Cross valid method: THROUGHOUT / ESU R: 0.113 / ESU R Free: 0.108 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 44.767 Å2
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Refinement step | Cycle: LAST / Resolution: 1.85→63.72 Å
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Refine LS restraints |
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