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Open data
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Basic information
Entry | Database: PDB / ID: 1hzf | ||||||
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Title | C4ADG FRAGMENT OF HUMAN COMPLEMENT FACTOR C4A | ||||||
![]() | COMPLEMENT FACTOR C4A | ||||||
![]() | IMMUNE SYSTEM / alpha-alpha 6 barrel | ||||||
Function / homology | ![]() : / detection of molecule of bacterial origin / opsonization / complement component C1q complex binding / complement binding / positive regulation of apoptotic cell clearance / regulation of complement activation / Activation of C3 and C5 / complement activation / endopeptidase inhibitor activity ...: / detection of molecule of bacterial origin / opsonization / complement component C1q complex binding / complement binding / positive regulation of apoptotic cell clearance / regulation of complement activation / Activation of C3 and C5 / complement activation / endopeptidase inhibitor activity / Initial triggering of complement / complement activation, classical pathway / Regulation of Complement cascade / Post-translational protein phosphorylation / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / carbohydrate binding / blood microparticle / inflammatory response / endoplasmic reticulum lumen / axon / innate immune response / neuronal cell body / synapse / dendrite / extracellular space / extracellular exosome / extracellular region / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | van den Elsen, J.M.H. / Martin, A. / Wong, V. / Clemenza, L. / Rose, D.R. / Isenman, D.E. | ||||||
![]() | ![]() Title: X-ray crystal structure of the C4d fragment of human complement component C4. Authors: van den Elsen, J.M. / Martin, A. / Wong, V. / Clemenza, L. / Rose, D.R. / Isenman, D.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.6 KB | Display | ![]() |
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PDB format | ![]() | 52.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.9 KB | Display | ![]() |
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Full document | ![]() | 434.6 KB | Display | |
Data in XML | ![]() | 14.1 KB | Display | |
Data in CIF | ![]() | 18.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1c3dS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39589.055 Da / Num. of mol.: 1 / Fragment: C4ADG Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: P01028, UniProt: P0C0L4*PLUS, classical-complement-pathway C3/C5 convertase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.8 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 4000, TRIS, magnesium chloride, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 6, 1999 / Details: OSMIC MIRRORS |
Radiation | Monochromator: OSMIC MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→26.84 Å / Num. all: 15682 / Num. obs: 15703 / % possible obs: 97.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.8 % / Biso Wilson estimate: 23.5 Å2 / Limit h max: 24 / Limit h min: 0 / Limit k max: 31 / Limit k min: 0 / Limit l max: 37 / Limit l min: 0 / Observed criterion F max: 729503.73 / Observed criterion F min: 0.89 / Rmerge(I) obs: 0.079 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 2.3→2.35 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.511 / Mean I/σ(I) obs: 2 / Num. unique all: 1057 / % possible all: 99.4 |
Reflection | *PLUS Highest resolution: 2.3 Å / Num. measured all: 43751 / Rmerge(I) obs: 0.079 |
Reflection shell | *PLUS % possible obs: 99.4 % / Num. unique obs: 1057 / Num. measured obs: 2898 / Rmerge(I) obs: 0.511 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1C3D Resolution: 2.3→26.84 Å / Rfactor Rfree error: 0.009 / Occupancy max: 1 / Occupancy min: 1 / Cross valid method: THROUGHOUT / σ(F): 1 / σ(I): 1 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: CNS bulk solvent model used / Bsol: 43.5389 Å2 / ksol: 0.348081 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 83.96 Å2 / Biso mean: 41.62 Å2 / Biso min: 19.61 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.3→26.84 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 500 Å / % reflection Rfree: 10 % / Rfactor Rfree: 0.233 / Rfactor Rwork: 0.215 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.32 / Rfactor Rwork: 0.29 |