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- PDB-5f8a: Crystal structure of the ternary EcoRV-DNA-Lu complex with unclea... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5f8a | ||||||
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Title | Crystal structure of the ternary EcoRV-DNA-Lu complex with uncleaved DNA substrate. Lanthanide binding to EcoRV-DNA complex inhibits cleavage. | ||||||
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![]() | HYDROLASE/DNA / Hydrolase / Protein-DNA complex / Lutetium / HYDROLASE-DNA complex | ||||||
Function / homology | ![]() type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() | ||||||
![]() | Sangani, S.S. / Kehr, A.D. / Sinha, K. / Rule, G.S. / Jen-Jacobson, L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein-DNA Complex. Authors: Sinha, K. / Sangani, S.S. / Kehr, A.D. / Rule, G.S. / Jen-Jacobson, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 137.7 KB | Display | ![]() |
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PDB format | ![]() | 102.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5hlkC ![]() 1b95S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
-DNA chain / Protein , 2 types, 4 molecules CDAB
#1: DNA chain | Mass: 3660.428 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Protein | Mass: 28559.158 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P04390, type II site-specific deoxyribonuclease |
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-Non-polymers , 4 types, 307 molecules ![](data/chem/img/LU.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.24 Å3/Da / Density % sol: 45.18 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, sitting drop / pH: 7.4 Details: 4.5 mg/mL (monomer), protein(dimer):DNA - 1:1, Enzyme:Well - 3uL:3uL, Well - 500uL of 100 mM HEPES pH 7.4, 8% Ethylene glycol, 4% Polyethylene glycol 8000, 10% glycerol. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS HTC / Detector: IMAGE PLATE / Date: Nov 18, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→36.673 Å / Num. obs: 51697 / % possible obs: 93 % / Redundancy: 3.9 % / Biso Wilson estimate: 21.698 Å2 / Rmerge(I) obs: 0.051 / Rsym value: 0.059 / Net I/σ(I): 14.7 |
Reflection shell | Resolution: 1.76→1.85 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.196 / Mean I/σ(I) obs: 4.4 / % possible all: 88.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1B95 Resolution: 1.76→36.673 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.955 / SU B: 2.494 / SU ML: 0.078 / Cross valid method: THROUGHOUT / ESU R: 0.127 / ESU R Free: 0.12 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.868 Å2
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Refinement step | Cycle: LAST / Resolution: 1.76→36.673 Å
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Refine LS restraints |
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