PDB-5els: Structure of the KH domain of T-STAR in complex with AAAUAA RNA

Basic information

• Entry: Database: PDB / ID: 5els
• Title: Structure of the KH domain of T-STAR in complex with AAAUAA RNA

Components

• KH domain-containing, RNA-binding, signal transduction-associated protein 3
• RNA (5'-R(P*AP*AP*AP*UP*AP*A)-3')

• Keywords: RNA BINDING PROTEIN / Protein - RNA complexes STAR protein Alternative splicing KH domain

Function / homology

Function:

PTK6 Regulates Proteins Involved in RNA Processing / regulation of alternative mRNA splicing, via spliceosome / mRNA processing / SH3 domain binding / protein domain specific binding / mRNA binding / protein-containing complex / RNA binding / nucleoplasm / identical protein binding / nucleus

Domain/homology:

Sam68, tyrosine-rich domain / KHDRBS, Qua1 domain / Qua1 domain / Tyrosine-rich domain of Sam68 / KH domain-containing BBP-like / KH domain / K Homology domain, type 1 superfamily / K Homology domain / K homology RNA-binding domain

Component:

RNA / KH domain-containing, RNA-binding, signal transduction-associated protein 3

• Biological species: Homo sapiens (human); synthetic construct (others)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.873 Å
• Authors: Dominguez, C. / Feracci, M.

Funding support

United Kingdom, 1items

Organization	Grant number	Country
Medical Research Council (United Kingdom)	G1000526	United Kingdom

• Citation: Journal: Nat Commun / Year: 2016; Title: Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.; Authors: Feracci, M. / Foot, J.N. / Grellscheid, S.N. / Danilenko, M. / Stehle, R. / Gonchar, O. / Kang, H.S. / Dalgliesh, C. / Meyer, N.H. / Liu, Y. / Lahat, A. / Sattler, M. / Eperon, I.C. / Elliott, D.J. / Dominguez, C.

History

Deposition	Nov 5, 2015	Deposition site: RCSB / Processing site: PDBE
Revision 1.0	Jan 13, 2016	Provider: repository / Type: Initial release
Revision 1.1	Jan 27, 2016	Group: Database references
Revision 1.2	Sep 13, 2017	Group: Author supporting evidence / Data collection / Category: diffrn_radiation_wavelength / pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3	May 8, 2024	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / struct_ncs_dom_lim Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

Assembly

Deposited unit

A: KH domain-containing, RNA-binding, signal transduction-associated protein 3

B: KH domain-containing, RNA-binding, signal transduction-associated protein 3

C: KH domain-containing, RNA-binding, signal transduction-associated protein 3

D: KH domain-containing, RNA-binding, signal transduction-associated protein 3

E: KH domain-containing, RNA-binding, signal transduction-associated protein 3

F: KH domain-containing, RNA-binding, signal transduction-associated protein 3

H: RNA (5'-R(P*AP*AP*AP*UP*AP*A)-3')

I: RNA (5'-R(P*AP*AP*AP*UP*AP*A)-3')

hetero molecules

Summary:

• 81.2 kDa, 8 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	81,191	11
Polymers	80,903	8
Non-polymers	288	3
Water	0	0

1

A: KH domain-containing, RNA-binding, signal transduction-associated protein 3

C: KH domain-containing, RNA-binding, signal transduction-associated protein 3

H: RNA (5'-R(P*AP*AP*AP*UP*AP*A)-3')

I: RNA (5'-R(P*AP*AP*AP*UP*AP*A)-3')

hetero molecules

Summary:

• defined by author
• 29.6 kDa, 4 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	29,607	5
Polymers	29,511	4
Non-polymers	96	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

B: KH domain-containing, RNA-binding, signal transduction-associated protein 3

F: KH domain-containing, RNA-binding, signal transduction-associated protein 3

hetero molecules

Summary:

• defined by author
• 25.9 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	25,888	4
Polymers	25,696	2
Non-polymers	192	2
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

3

D: KH domain-containing, RNA-binding, signal transduction-associated protein 3

E: KH domain-containing, RNA-binding, signal transduction-associated protein 3

Summary:

• defined by author
• 25.7 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	25,696	2
Polymers	25,696	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	93.730, 162.220, 113.040
Angle α, β, γ (deg.)	90.000, 90.000, 90.000
Int Tables number	20
Space group name H-M	C2221

Noncrystallographic symmetry (NCS)

NCS domain:

ID	Ens-ID	Details
1	1	(chain A and (resseq 48:51 or resseq 53:58 or resseq...
2	1	(chain B and (resseq 48:51 or resseq 53:58 or resseq...
3	1	(chain C and (resseq 48:51 or resseq 53:58 or resseq...
4	1	(chain D and (resseq 48:51 or resseq 53:58 or resseq...
5	1	(chain E and (resseq 48:51 or resseq 53:58 or resseq...
6	1	(chain F and (resseq 48:51 or resseq 53:58 or resseq...

NCS domain segments:

Ens-ID: 1

Dom-ID	Component-ID	Beg auth comp-ID	Beg label comp-ID	End auth comp-ID	End label comp-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	GLY	GLY	ASN	ASN	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	48 - 51	1 - 4
1	2	ASN	ASN	GLN	GLN	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	53 - 58	6 - 11
1	3	VAL	VAL	VAL	VAL	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	60 - 64	13 - 17
1	4	PHE	PHE	PRO	PRO	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	67 - 68	20 - 21
1	5	PHE	PHE	GLY	GLY	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	70 - 74	23 - 27
1	6	LEU	LEU	PRO	PRO	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	76 - 79	29 - 32
1	7	GLY	GLY	LEU	LEU	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	81 - 84	34 - 37
1	8	LEU	LEU	GLN	GLN	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	87 - 88	40 - 41
1	9	THR	THR	LYS	LYS	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	91 - 94	44 - 47
1	10	SER	SER	GLY	GLY	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	96 - 99	49 - 52
1	11	GLY	GLY	MET	MET	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	101 - 103	54 - 56
1	12	ASP	ASP	ASP	ASP	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	105	58
1	13	ALA	ALA	ALA	ALA	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	107	60
1	14	GLU	GLU	GLU	GLU	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	109 - 110	62 - 63
1	15	LEU	LEU	ARG	ARG	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	112 - 113	65 - 66
1	16	SER	SER	GLY	GLY	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	115 - 116	68 - 69
1	17	ALA	ALA	ALA	ALA	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	118 - 142	71 - 95
1	18	MET	MET	LEU	LEU	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	144 - 148	97 - 101
1	19	GLU	GLU	LYS	LYS	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	150 - 152	103 - 105
1	20	PHE	PHE	PRO	PRO	(chain A and (resseq 48:51 or resseq 53:58 or resseq...	A	A	154 - 157	107 - 110
2	1	GLY	GLY	ASN	ASN	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	48 - 51	1 - 4
2	2	ASN	ASN	GLN	GLN	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	53 - 58	6 - 11
2	3	VAL	VAL	VAL	VAL	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	60 - 64	13 - 17
2	4	PHE	PHE	PRO	PRO	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	67 - 68	20 - 21
2	5	PHE	PHE	GLY	GLY	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	70 - 74	23 - 27
2	6	LEU	LEU	PRO	PRO	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	76 - 79	29 - 32
2	7	GLY	GLY	LEU	LEU	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	81 - 84	34 - 37
2	8	LEU	LEU	GLN	GLN	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	87 - 88	40 - 41
2	9	THR	THR	LYS	LYS	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	91 - 94	44 - 47
2	10	SER	SER	GLY	GLY	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	96 - 99	49 - 52
2	11	GLY	GLY	MET	MET	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	101 - 103	54 - 56
2	12	ASP	ASP	ASP	ASP	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	105	58
2	13	ALA	ALA	ALA	ALA	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	107	60
2	14	GLU	GLU	GLU	GLU	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	109 - 110	62 - 63
2	15	LEU	LEU	ARG	ARG	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	112 - 113	65 - 66
2	16	SER	SER	GLY	GLY	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	115 - 116	68 - 69
2	17	ALA	ALA	ALA	ALA	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	118 - 142	71 - 95
2	18	MET	MET	LEU	LEU	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	144 - 148	97 - 101
2	19	GLU	GLU	LYS	LYS	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	150 - 152	103 - 105
2	20	PHE	PHE	PRO	PRO	(chain B and (resseq 48:51 or resseq 53:58 or resseq...	B	B	154 - 157	107 - 110
3	1	GLY	GLY	ASN	ASN	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	48 - 51	1 - 4
3	2	ASN	ASN	GLN	GLN	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	53 - 58	6 - 11
3	3	VAL	VAL	VAL	VAL	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	60 - 64	13 - 17
3	4	PHE	PHE	PRO	PRO	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	67 - 68	20 - 21
3	5	PHE	PHE	GLY	GLY	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	70 - 74	23 - 27
3	6	LEU	LEU	PRO	PRO	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	76 - 79	29 - 32
3	7	GLY	GLY	LEU	LEU	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	81 - 84	34 - 37
3	8	LEU	LEU	GLN	GLN	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	87 - 88	40 - 41
3	9	THR	THR	LYS	LYS	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	91 - 94	44 - 47
3	10	SER	SER	GLY	GLY	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	96 - 99	49 - 52
3	11	GLY	GLY	MET	MET	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	101 - 103	54 - 56
3	12	ASP	ASP	ASP	ASP	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	105	58
3	13	ALA	ALA	ALA	ALA	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	107	60
3	14	GLU	GLU	GLU	GLU	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	109 - 110	62 - 63
3	15	LEU	LEU	ARG	ARG	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	112 - 113	65 - 66
3	16	SER	SER	GLY	GLY	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	115 - 116	68 - 69
3	17	ALA	ALA	ALA	ALA	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	118 - 142	71 - 95
3	18	MET	MET	LEU	LEU	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	144 - 148	97 - 101
3	19	GLU	GLU	LYS	LYS	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	150 - 152	103 - 105
3	20	PHE	PHE	PRO	PRO	(chain C and (resseq 48:51 or resseq 53:58 or resseq...	C	C	154 - 157	107 - 110
4	1	GLY	GLY	ASN	ASN	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	48 - 51	1 - 4
4	2	ASN	ASN	GLN	GLN	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	53 - 58	6 - 11
4	3	VAL	VAL	VAL	VAL	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	60 - 64	13 - 17
4	4	PHE	PHE	PRO	PRO	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	67 - 68	20 - 21
4	5	PHE	PHE	GLY	GLY	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	70 - 74	23 - 27
4	6	LEU	LEU	PRO	PRO	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	76 - 79	29 - 32
4	7	GLY	GLY	LEU	LEU	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	81 - 84	34 - 37
4	8	LEU	LEU	GLN	GLN	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	87 - 88	40 - 41
4	9	THR	THR	LYS	LYS	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	91 - 94	44 - 47
4	10	SER	SER	GLY	GLY	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	96 - 99	49 - 52
4	11	GLY	GLY	MET	MET	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	101 - 103	54 - 56
4	12	ASP	ASP	ASP	ASP	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	105	58
4	13	ALA	ALA	ALA	ALA	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	107	60
4	14	GLU	GLU	GLU	GLU	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	109 - 110	62 - 63
4	15	LEU	LEU	ARG	ARG	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	112 - 113	65 - 66
4	16	SER	SER	GLY	GLY	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	115 - 116	68 - 69
4	17	ALA	ALA	ALA	ALA	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	118 - 142	71 - 95
4	18	MET	MET	LEU	LEU	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	144 - 148	97 - 101
4	19	GLU	GLU	LYS	LYS	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	150 - 152	103 - 105
4	20	PHE	PHE	PRO	PRO	(chain D and (resseq 48:51 or resseq 53:58 or resseq...	D	D	154 - 157	107 - 110
5	1	GLY	GLY	ASN	ASN	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	48 - 51	1 - 4
5	2	ASN	ASN	GLN	GLN	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	53 - 58	6 - 11
5	3	VAL	VAL	VAL	VAL	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	60 - 64	13 - 17
5	4	PHE	PHE	PRO	PRO	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	67 - 68	20 - 21
5	5	PHE	PHE	GLY	GLY	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	70 - 74	23 - 27
5	6	LEU	LEU	PRO	PRO	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	76 - 79	29 - 32
5	7	GLY	GLY	LEU	LEU	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	81 - 84	34 - 37
5	8	LEU	LEU	GLN	GLN	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	87 - 88	40 - 41
5	9	THR	THR	LYS	LYS	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	91 - 94	44 - 47
5	10	SER	SER	GLY	GLY	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	96 - 99	49 - 52
5	11	GLY	GLY	MET	MET	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	101 - 103	54 - 56
5	12	ASP	ASP	ASP	ASP	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	105	58
5	13	ALA	ALA	ALA	ALA	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	107	60
5	14	GLU	GLU	GLU	GLU	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	109 - 110	62 - 63
5	15	LEU	LEU	ARG	ARG	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	112 - 113	65 - 66
5	16	SER	SER	GLY	GLY	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	115 - 116	68 - 69
5	17	ALA	ALA	ALA	ALA	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	118 - 142	71 - 95
5	18	MET	MET	LEU	LEU	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	144 - 148	97 - 101
5	19	GLU	GLU	LYS	LYS	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	150 - 152	103 - 105
5	20	PHE	PHE	PRO	PRO	(chain E and (resseq 48:51 or resseq 53:58 or resseq...	E	E	154 - 157	107 - 110
6	1	GLY	GLY	ASN	ASN	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	48 - 51	1 - 4
6	2	ASN	ASN	GLN	GLN	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	53 - 58	6 - 11
6	3	VAL	VAL	VAL	VAL	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	60 - 64	13 - 17
6	4	PHE	PHE	PRO	PRO	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	67 - 68	20 - 21
6	5	PHE	PHE	GLY	GLY	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	70 - 74	23 - 27
6	6	LEU	LEU	PRO	PRO	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	76 - 79	29 - 32
6	7	GLY	GLY	LEU	LEU	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	81 - 84	34 - 37
6	8	LEU	LEU	GLN	GLN	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	87 - 88	40 - 41
6	9	THR	THR	LYS	LYS	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	91 - 94	44 - 47
6	10	SER	SER	GLY	GLY	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	96 - 99	49 - 52
6	11	GLY	GLY	MET	MET	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	101 - 103	54 - 56
6	12	ASP	ASP	ASP	ASP	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	105	58
6	13	ALA	ALA	ALA	ALA	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	107	60
6	14	GLU	GLU	GLU	GLU	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	109 - 110	62 - 63
6	15	LEU	LEU	ARG	ARG	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	112 - 113	65 - 66
6	16	SER	SER	GLY	GLY	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	115 - 116	68 - 69
6	17	ALA	ALA	ALA	ALA	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	118 - 142	71 - 95
6	18	MET	MET	LEU	LEU	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	144 - 148	97 - 101
6	19	GLU	GLU	LYS	LYS	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	150 - 152	103 - 105
6	20	PHE	PHE	PRO	PRO	(chain F and (resseq 48:51 or resseq 53:58 or resseq...	F	F	154 - 157	107 - 110

Components

#1: Protein

A B C D E F
KH domain-containing, RNA-binding, signal transduction-associated protein 3 / RNA-binding protein T-Star / Sam68-like mammalian protein 2 / SLM-2 / Sam68-like phosphotyrosine protein

Details:

Mass: 12848.066 Da / Num. of mol.: 6 / Fragment: RNA binding protein, UNP residues 50-160
Source method: isolated from a genetically manipulated source
Details: N-terminus GA residues from tag. / Source: (gene. exp.) Homo sapiens (human) / Gene: KHDRBS3, SALP, SLM2 / Plasmid: pLEICS 03 / Production host: Escherichia coli (E. coli) / References: UniProt: O75525

#2: RNA chain

H I RNA (5'-R(P*AP*AP*AP*UP*AP*A)-3')

Details:

Mass: 1907.237 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

#3: Chemical

A-201 B-201 B-202 ChemComp-SO4 / SULFATE ION

Details:

Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO₄

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.66 ų/Da / Density % sol: 53.68 %
• Crystal grow: Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: Lithium sulphate, Tris

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9173 Å
• Detector: Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Feb 3, 2012
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.9173 Å / Relative weight: 1
• Reflection twin: Operator: 1/2*h+1/2*k,3/2*h-1/2*k,-l / Fraction: 0.25
• Reflection: Resolution: 2.873→46.865 Å / Num. all: 20014 / Num. obs: 20014 / % possible obs: 99.8 % / Redundancy: 6.9 % / R_pim(I) all: 0.078 / R_rim(I) all: 0.206 / R_sym value: 0.174 / Net I/av σ(I): 3.5 / Net I/σ(I): 8.1 / Num. measured all: 137466

Reflection shell

Diffraction-ID: 1 / Rejects: _

Resolution (Å)	Redundancy (%)	Rmerge(I) obs	Mean I/σ(I) obs	Num. measured all	Num. unique all	Rpim(I) all	Rsym value	Net I/σ(I) obs	% possible all
2.87-2.95	7.2	0.745	1	10690	1475	0.321	0.745	2.4	100
2.95-3.03	7.2	0.599	1.3	10309	1435	0.258	0.599	3.1	99.8
3.03-3.12	7.1	0.51	1.5	9766	1368	0.222	0.51	3.6	99.8
3.12-3.21	7.2	0.426	1.8	9655	1344	0.186	0.426	4.2	99.9
3.21-3.32	6.9	0.358	2.1	8927	1300	0.159	0.358	4.7	100
3.32-3.43	6.6	0.283	2.7	8301	1251	0.13	0.283	5.8	99.6
3.43-3.56	6	0.233	3.3	7386	1223	0.115	0.233	6.4	99.6
3.56-3.71	6	0.186	4	7040	1174	0.093	0.186	7.8	99.8
3.71-3.87	7.3	0.178	4.2	8411	1150	0.078	0.178	9.5	100
3.87-4.06	7.2	0.153	4.8	7821	1086	0.067	0.153	10.8	99.8
4.06-4.28	7.2	0.139	4.8	7370	1029	0.064	0.139	11.4	99.9
4.28-4.54	7.1	0.131	5.5	6783	955	0.058	0.131	11.8	100
4.54-4.86	7.1	0.123	5.7	6585	928	0.055	0.123	12.3	99.9
4.86-5.25	6.9	0.134	5.4	6087	883	0.06	0.134	11.2	99.9
5.25-5.75	6.7	0.124	5.8	5161	773	0.058	0.124	11.1	100
5.75-6.42	6.5	0.122	5.6	4734	731	0.057	0.122	11.3	99.6
6.42-7.42	5.5	0.101	6.5	3565	644	0.053	0.101	11.8	99.5
7.42-9.08	7.3	0.082	7.7	4079	558	0.035	0.082	15.9	100
9.08-12.85	7.1	0.068	9.4	3115	441	0.028	0.068	17	100
12.85-46.865	6.3	0.062	8.3	1681	266	0.028	0.062	17	98.7

Phasing

• Phasing: Method: molecular replacement

Processing

Software

Name	Version	Classification
PHENIX		refinement
SCALA	3.3.20	data scaling
PHASER		phasing
PDB_EXTRACT	3.15	data extraction
xia2		data reduction

Refinement

Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.873→46.865 Å / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 29.47 / Stereochemistry target values: TWIN_LSQ_F

	Rfactor	Num. reflection	% reflection	Selection details
Rfree	0.2358	1021	5.11 %	RAMDOM
Rwork	0.1875	18918	-	-
obs	0.1897	19995	99.76 %	-

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso max: 198.12 Ų / B_iso mean: 52.3359 Ų / B_iso min: 11.2 Ų

Refinement step

Cycle: final / Resolution: 2.873→46.865 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	5356	260	15	0	5631
Biso mean	-	-	58.75	-	-
Num. residues	-	-	-	-	684

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.013	5760
X-RAY DIFFRACTION	f_angle_d	1.461	7756
X-RAY DIFFRACTION	f_chiral_restr	0.076	840
X-RAY DIFFRACTION	f_plane_restr	0.012	948
X-RAY DIFFRACTION	f_dihedral_angle_d	18.232	3538

Refine LS restraints NCS

Ens-ID	Dom-ID	Auth asym-ID	Number	Refine-ID	Rms	Type
1	1	A	2116	X-RAY DIFFRACTION	13.758	TORSIONAL
1	2	B	2116	X-RAY DIFFRACTION	13.758	TORSIONAL
1	3	C	2116	X-RAY DIFFRACTION	13.758	TORSIONAL
1	4	D	2116	X-RAY DIFFRACTION	13.758	TORSIONAL
1	5	E	2116	X-RAY DIFFRACTION	13.758	TORSIONAL
1	6	F	2116	X-RAY DIFFRACTION	13.758	TORSIONAL

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 7

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Num. reflection all	% reflection obs (%)
2.8732-3.0244	0.3236	144	0.2583	2690	2834	95
3.0244-3.2133	0.2771	144	0.2384	2652	2796	95
3.2133-3.4606	0.273	141	0.2215	2666	2807	95
3.4606-3.8074	0.2484	164	0.1866	2643	2807	94
3.8074-4.3549	0.2131	144	0.165	2711	2855	95
4.3549-5.4736	0.2111	142	0.162	2729	2871	95
5.4736-22.5126	0.191	141	0.1685	2827	2968	95

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.1565	-0.4758	1.0262	0.5994	-0.575	0.8336	0.0463	-0.1322	0.1123	0.1784	-0.1148	-0.1116	0.1628	-0.2209	0.0484	0.2263	0.0023	-0.0727	0.1438	0.0269	0.1793	129.1059	34.1578	126.0307
2	2.088	0.4727	0.2318	1.7488	-0.7278	2.7006	0.021	-0.3979	-0.4438	-0.2422	-0.1687	-0.3575	-0.2868	0.1522	-0.0301	0.307	-0.0891	0.0027	0.1979	0.019	0.09	123.8663	18.5951	132.7831
3	1.3132	-0.3514	0.3058	0.2572	-0.0521	0.9793	0.1327	0.0875	0.122	0.0024	0.0025	0.0545	-0.1926	0.2567	0.0865	0.2136	0.0243	0.0375	0.1465	0.0315	0.1423	130.2314	36.8637	121.1921
4	0.1024	-0.0905	-0.0273	0.1052	0.041	0.0159	0.1578	-0.3724	0.2919	0.3647	0.1875	0.2607	-0.0099	-0.3441	0.104	0.7489	0.126	0.0753	0.523	0.0278	0.3935	125.4462	-14.0435	171.8645
5	1.2905	-0.6293	0.3558	2.1402	0.1063	0.1474	0.2629	-0.3456	-0.4701	0.2971	0.0179	0.042	0.1206	-0.2689	-0.0883	0.3229	-0.0609	0.0012	0.4245	0.1538	0.2466	122.4622	0.2617	163.7517
6	0.2757	-0.1019	0.2871	0.3914	-0.0078	0.3197	-0.0481	-0.0646	-0.0068	0.1757	-0.2191	0.0635	-0.0022	-0.2268	-0.3626	-0.1314	-0.0397	0.5563	0.3212	0.1513	-0.0781	123.3769	20.78	153.7128
7	0.9474	0.2776	1.427	2.4757	1.0716	2.3231	0.2201	0.1758	0.0549	-0.4251	0.0344	-0.1297	-0.1355	0.5602	0.2808	0.3019	-0.1969	0.2261	0.196	0.1482	-0.1224	128.6257	12.4385	148.988
8	1.0288	-0.039	-0.1287	1.1059	0.7422	1.0693	0.04	0.016	-0.238	-0.0463	-0.1929	-0.0395	0.4278	0.0522	-0.1649	0.5552	0.152	0.0751	0.0595	0.1387	0.667	133.9229	2.8888	149.4528
9	2.3303	-2.8989	1.485	4.2643	-1.0545	1.9305	-0.243	-0.1193	-0.1046	-0.1578	-0.2891	0.0852	0.0747	-0.1467	-0.0028	0.343	0.0088	0.1193	0.1569	0.0953	0.3463	125.9588	1.3131	154.8414
10	1.5171	0.5097	-0.177	0.7313	-0.2427	0.894	-0.1001	-0.1612	-0.0263	-0.0963	-0.0762	0.1147	0.0001	-0.1301	-0.1715	0.3916	0.2308	-0.4023	0.6572	0.0134	0.671	116.6451	12.1791	146.8602
11	0.6661	0.3295	0.6263	3.3352	0.3995	0.9574	-0.0964	0.3362	-0.2661	0.0865	-0.1705	0.4387	0.2554	-0.2301	0.011	0.8647	-0.0111	-0.2299	0.6058	-0.0237	0.5051	108.0379	10.2781	145.7635
12	3.6798	1.3082	-0.6946	1.6893	-0.8988	0.5168	0.1541	0.1013	-0.3263	-0.1839	0.082	0.4065	0.2221	0.0171	-0.0346	0.3395	0.0073	-0.0752	0.4474	0.2084	0.4293	111.0019	6.2158	153.2143
13	-0.0044	-0.0088	-0.0162	0.0039	0.132	1.6283	0.0963	0.151	0.3124	0.1558	-0.2589	0.0389	0.4483	-0.136	-0.0444	0.1757	0.0699	-0.1439	0.2353	0.0606	0.36	128.8457	-0.5015	163.3881
14	1.4182	0.0052	0.2259	2.9892	-0.7612	1.3808	-0.1067	0.1527	0.0656	-0.0394	-0.1219	-0.3003	0.3165	0.2039	0.0504	0.1901	-0.0177	-0.0635	0.2084	0.0616	0.2875	133.1207	16.8825	154.4439
15	0.0584	-0.0471	0.0123	0.05	-0.0559	0.1677	0.0578	-0.1374	0.0307	0.1257	-0.0683	0.0287	0.0209	0.12	-0.0322	0.6529	-0.0732	0.2759	0.9616	-0.4225	0.2702	98.4764	52.7048	170.9656
16	0.8807	-0.8566	-0.9423	1.0822	0.6441	0.967	-0.0491	0.1315	-0.2204	0.0478	-0.0927	0.1813	-0.1676	-0.1421	0.0387	0.1536	-0.0066	-0.0187	0.2211	-0.03	0.1038	117.3036	39.6959	153.139
17	1.8317	0.616	0.3771	2.2064	-0.2044	1.605	-0.3126	0.2689	0.079	-0.5301	-0.2165	0.0158	-0.4207	0.1861	-0.2675	0.1303	-0.0142	0.0661	0.3213	-0.0846	0.1325	119.0327	46.1463	149.8898
18	1.8401	0.452	-0.6575	0.7081	-0.4507	1.3832	-0.0469	0.0864	0.1048	-0.019	0.0564	-0.0435	-0.0111	0.125	0.0345	0.7384	-0.056	0.4501	0.6574	0.1457	0.4276	129.4483	55.4967	144.8376
19	0.7278	-0.3548	-0.5041	0.4195	0.3625	0.6288	0.0391	0.1257	0.1205	-0.0705	-0.0526	0.0867	-0.0309	0.0161	0.0008	0.2145	0.0018	0.0183	0.1815	0.0279	0.1503	116.0448	43.9336	157.4025
20	0.5818	-0.0545	-0.8597	1.8876	0.869	1.5406	-0.1621	-0.056	0.1393	0.1369	0.1917	-0.2472	0.3502	-0.1633	-0.0398	0.4888	-0.0426	0.1897	0.3387	-0.0342	0.2443	116.0433	17.0323	116.7381
21	4.1019	-1.7287	0.7082	2.8379	0.4038	0.358	0.037	-0.2742	-0.2296	0.1737	0.1027	0.5099	-0.098	-0.179	-0.0701	0.5055	-0.1639	0.4144	0.6343	-0.1674	0.9405	91.2785	30.028	119.0483
22	3.5641	2.2645	-1.5676	2.6939	0.3602	2.1475	0.0861	-0.0189	-0.3248	0.4509	-0.1094	-0.2225	0.1741	0.4768	0.159	0.8885	-0.2576	0.3689	0.5185	-0.0171	0.7614	94.8556	20.7063	130.8688
23	0.151	0.202	-0.1223	2.331	0.094	0.2905	-0.1088	-0.0478	0.1678	0.3262	0.1199	0.3638	0.3438	0.0053	-0.031	0.5571	-0.0829	0.1026	0.3884	-0.0029	0.4256	103.7584	15.9742	127.0568
24	3.3109	0.8002	-0.1368	2.0379	-0.3861	1.8967	-0.164	-0.245	0.1198	0.0568	-0.3928	0.3044	0.1748	-0.6535	0.1328	0.3488	0.1305	0.0159	0.5574	-0.1773	0.3555	104.7428	38.4829	130.0839
25	0.3846	0.0572	0.1413	1.3809	0.4662	0.8551	0.1414	-0.0558	-0.1334	-0.0206	-0.1931	0.0255	0.1521	-0.4372	0.0589	0.2726	-0.1121	0.0391	0.255	0.0064	0.308	105.9328	20.8216	120.0954
26	0.0674	0.3997	0.0571	2.4258	0.8266	4.4386	-0.0424	0.1417	-0.12	-0.2869	-0.0592	0.0798	0.1782	-0.2663	0.1824	0.6297	0.0707	0.0484	1.0168	-0.2547	0.2806	100.5007	23.4833	110.1664
27	4.7072	-0.3196	-0.1274	2.091	0.1817	2.1008	0.0638	0.0086	-0.0773	0.006	0.1335	-0.2261	0.1623	-0.2915	-0.0224	0.6327	-0.0907	0.0071	0.2467	0.0325	0.2646	116.0992	18.0705	106.9287
28	0.6163	-0.541	-0.1694	3.0031	1.8164	1.1405	-0.0171	0.2707	-0.5816	0.0578	0.099	-0.3302	0.625	0.6754	0.0727	1.0768	0.1753	0.5557	0.7207	-0.1211	0.8879	134.6324	4.2589	105.6245
29	2.1271	-1.1637	4.1305	0.6611	-2.2186	8.0662	-0.0819	0.2003	0.1912	-0.1614	0.2092	-0.0718	-0.0446	0.4279	0.0284	1.1594	-0.0611	0.1074	0.5513	0.0837	0.9902	127.0664	3.7535	97.6563
30	4.3893	-2.1976	-2.5273	2.7096	1.667	1.6633	0.439	0.4412	-0.1355	-0.6203	-0.0086	-0.0038	-0.0115	-0.0293	-0.1103	0.7078	-0.0305	-0.0293	0.4625	-0.0428	0.8174	116.3246	4.4843	94.4902
31	0.3042	0.2507	0.0117	1.0039	-0.1314	0.9323	0.0142	0.3286	0.014	-0.5492	-0.0742	-0.2796	-0.324	-0.01	0.1617	0.6495	-0.2108	0.2492	0.3768	-0.1792	0.5728	126.4133	13.6937	96.8936
32	0.568	-0.0424	-0.2033	0.4792	-0.2254	0.2114	-0.0456	0.0626	0.1159	0.0264	-0.0776	-0.001	0.4199	0.367	0.0435	0.4583	-0.102	0.114	0.5275	-0.1274	0.4532	132.892	23.4141	96.8411
33	1.4031	0.2098	0.2916	1.6452	-0.589	1.8328	-0.0157	0.0212	-0.5328	0.0301	-0.2832	-0.165	0.1274	0.0506	0.0998	0.5372	-0.1551	0.0084	0.2874	-0.0048	0.386	115.0835	10.1071	106.2823
34	1.568	-0.0315	-0.5351	0.1435	0.2812	1.9808	0.5014	0.1114	-0.1027	0.0011	0.3431	0.2418	-0.1241	-0.235	-0.1026	0.3451	0.0893	-0.0636	0.7876	0.105	0.5867	106.2786	3.1137	165.345
35	0.1487	-0.1964	0.7927	0.2503	-1.0268	4.1862	0.0017	-0.2648	-0.2099	0.2461	-0.1015	0.0477	0.1487	-0.0572	-0.0748	1.1275	-0.121	0.3642	0.9849	0.3496	0.7821	105.5621	-25.0522	161.4699
36	2.1173	0.2571	0.1864	1.0892	-0.4151	0.7047	-0.0629	0.005	-0.1589	-0.1844	-0.1029	-0.1743	0.1315	0.0409	0.0774	0.6732	-0.3814	0.3929	1.2624	-0.1028	0.9858	99.3711	-17.3209	150.9423
37	0.1627	0.199	0.0148	0.5704	0.2682	0.1837	-0.0433	-0.1722	0.1198	0.2127	-0.0239	1.0527	-0.2982	-0.7024	0.1692	0.5489	-0.0971	0.049	1.1376	0.141	0.8946	98.5541	-7.1144	154.5145
38	0.0729	-0.0943	0.0219	0.8588	0.7296	0.7634	-0.1705	0.1501	-0.283	-0.2294	0.1431	-0.1528	0.6624	-0.314	0.0287	0.4742	-0.092	-0.0075	0.7376	-0.045	0.7173	118.5502	-15.4038	153.0707
39	1.744	0.8374	-0.2914	0.7623	-0.7464	1.8529	-0.0427	0.6521	-0.0823	-0.2417	0.0236	-0.1371	0.3636	-0.7555	0.0308	0.1865	-0.087	-0.0048	1.0656	0.196	0.6056	99.3705	-7.1833	163.0954
40	1.4479	-0.2623	-0.7931	0.6726	-0.0908	0.5133	0.1198	-0.1247	-0.0305	0.5899	-0.1031	0.2367	-0.1441	-0.191	-0.0209	0.2916	-0.0065	-0.0336	0.4848	-0.0942	0.2487	125.0115	36.364	140.2651
41	0.4539	-0.0569	-0.0155	1.2131	0.5208	0.2304	0.0418	0.3707	0.0594	-0.0208	0.0078	-0.1005	0.1192	0.0293	-0.0141	0.5152	0.1213	0.0091	0.402	0.0746	0.1645	120.4237	38.8372	141.4239

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 48 through 98 )	A	0
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 99 through 127 )	A	0
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 128 through 160 )	A	0
4	X-RAY DIFFRACTION	4	chain 'B' and (resid 48 through 52 )	B	0
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 53 through 61 )	B	0
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 62 through 71 )	B	0
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 72 through 78 )	B	0
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 79 through 91 )	B	0
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 92 through 98 )	B	0
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 99 through 105 )	B	0
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 106 through 115 )	B	0
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 116 through 127 )	B	0
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 128 through 150 )	B	0
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 151 through 160 )	B	0
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 48 through 52 )	C	0
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 53 through 91 )	C	0
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 92 through 105 )	C	0
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 106 through 114 )	C	0
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 115 through 160 )	C	0
20	X-RAY DIFFRACTION	20	chain 'D' and (resid 48 through 61 )	D	0
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 62 through 72 )	D	0
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 73 through 82 )	D	0
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 83 through 98 )	D	0
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 99 through 117 )	D	0
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 118 through 158 )	D	0
26	X-RAY DIFFRACTION	26	chain 'E' and (resid 48 through 52 )	E	0
27	X-RAY DIFFRACTION	27	chain 'E' and (resid 53 through 61 )	E	0
28	X-RAY DIFFRACTION	28	chain 'E' and (resid 62 through 71 )	E	0
29	X-RAY DIFFRACTION	29	chain 'E' and (resid 72 through 78 )	E	0
30	X-RAY DIFFRACTION	30	chain 'E' and (resid 79 through 91 )	E	0
31	X-RAY DIFFRACTION	31	chain 'E' and (resid 92 through 106 )	E	0
32	X-RAY DIFFRACTION	32	chain 'E' and (resid 107 through 127 )	E	0
33	X-RAY DIFFRACTION	33	chain 'E' and (resid 128 through 157 )	E	0
34	X-RAY DIFFRACTION	34	chain 'F' and (resid 48 through 61 )	F	0
35	X-RAY DIFFRACTION	35	chain 'F' and (resid 62 through 71 )	F	0
36	X-RAY DIFFRACTION	36	chain 'F' and (resid 72 through 81 )	F	0
37	X-RAY DIFFRACTION	37	chain 'F' and (resid 82 through 98 )	F	0
38	X-RAY DIFFRACTION	38	chain 'F' and (resid 99 through 127 )	F	0
39	X-RAY DIFFRACTION	39	chain 'F' and (resid 128 through 159 )	F	0
40	X-RAY DIFFRACTION	40	chain 'H' and (resid -1 through 4 )	H	0
41	X-RAY DIFFRACTION	41	chain 'I' and (resid 0 through 5 )	I	0