[English] 日本語
![](img/lk-miru.gif)
- PDB-5ej0: The vaccinia virus H3 envelope protein, a major target of neutral... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 5ej0 | ||||||
---|---|---|---|---|---|---|---|
Title | The vaccinia virus H3 envelope protein, a major target of neutralizing antibodies, exhibits a glycosyltransferase fold and binds UDP-Glucose | ||||||
![]() | Envelope protein H3 | ||||||
![]() | VIRAL PROTEIN / H3 / vaccinia virus / poxvirus / glycosyl transferase | ||||||
Function / homology | ![]() membrane => GO:0016020 / symbiont entry into host cell / viral envelope / virion attachment to host cell / virion membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Singh, K. / Gittis, A.G. / Gitti, R.K. / Ostazesky, S.A. / Su, H.P. / Garboczi, D.N. | ||||||
![]() | ![]() Title: The Vaccinia Virus H3 Envelope Protein, a Major Target of Neutralizing Antibodies, Exhibits a Glycosyltransferase Fold and Binds UDP-Glucose. Authors: Singh, K. / Gittis, A.G. / Gitti, R.K. / Ostazeski, S.A. / Su, H.P. / Garboczi, D.N. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 64.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 44.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 500.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 507.9 KB | Display | |
Data in XML | ![]() | 13 KB | Display | |
Data in CIF | ![]() | 17.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 27486.207 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: Western Reserve / Gene: VACWR101, H3L / Plasmid: pNAN / Production host: ![]() ![]() |
---|
-Non-polymers , 10 types, 116 molecules ![](data/chem/img/DHL.gif)
![](data/chem/img/PG0.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/POL.gif)
![](data/chem/img/PDO.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/EOH.gif)
![](data/chem/img/MOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PG0.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/POL.gif)
![](data/chem/img/PDO.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/EOH.gif)
![](data/chem/img/MOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-DHL / | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
#3: Chemical | ChemComp-PG0 / #4: Chemical | #5: Chemical | ChemComp-POL / | #6: Chemical | #7: Chemical | ChemComp-EDO / #8: Chemical | #9: Chemical | ChemComp-MOH / #10: Chemical | ChemComp-MG / | #11: Water | ChemComp-HOH / | |
-Details
Sequence details | AUTHORS HAVE INDICATED THAT THE SEQUENCE IN THE UNPROT ENTRY P07240 IS INCORRECT |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.97 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 100 mM citric acid, 10-20% PEG 6000, 5% 1,3-propanediol and mixture of low molecular mass alcohols |
-Data collection
Diffraction | Mean temperature: 95 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 28, 2005 |
Radiation | Monochromator: Si(111) double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→49.22 Å / Num. obs: 21213 / % possible obs: 99.81 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 4.79 % / Rmerge(I) obs: 0.059 / Net I/σ(I): 18.54 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 97.01 Å2 / Biso mean: 33.3324 Å2 / Biso min: 11.64 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.9→49.216 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 12
|