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- PDB-5e62: HEF-mut with Tr323 complex -

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Basic information

Entry
Database: PDB / ID: 5.0E+62
TitleHEF-mut with Tr323 complex
Components(Hemagglutinin- ...) x 2
KeywordsHYDROLASE / influenza / complex / HEF
Function / homology
Function and homology information


sialate O-acetylesterase activity / sialate O-acetylesterase / host cell surface receptor binding / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / symbiont entry into host cell / virion attachment to host cell / host cell plasma membrane / virion membrane / membrane
Similarity search - Function
Ubiquitin Ligase Nedd4; Chain: W; - #20 / Haemagglutinin stalk, influenza C / Influenza C hemagglutinin stalk / Haemagglutinin-esterase glycoprotein, haemagglutinin domain / Haemagglutinin-esterase glycoprotein, core / Hemagglutinin domain of haemagglutinin-esterase-fusion glycoprotein / Hemagglutinin esterase / Ubiquitin Ligase Nedd4; Chain: W; / Hemagglutinin Ectodomain; Chain B - #10 / Hemagglutinin Ectodomain; Chain B ...Ubiquitin Ligase Nedd4; Chain: W; - #20 / Haemagglutinin stalk, influenza C / Influenza C hemagglutinin stalk / Haemagglutinin-esterase glycoprotein, haemagglutinin domain / Haemagglutinin-esterase glycoprotein, core / Hemagglutinin domain of haemagglutinin-esterase-fusion glycoprotein / Hemagglutinin esterase / Ubiquitin Ligase Nedd4; Chain: W; / Hemagglutinin Ectodomain; Chain B - #10 / Hemagglutinin Ectodomain; Chain B / Haemagglutinin / Haemagglutinin, influenzavirus A/B / Viral capsid/haemagglutinin protein / Single Sheet / Alpha-Beta Complex / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Hemagglutinin-esterase-fusion glycoprotein
Similarity search - Component
Biological speciesInfluenza D virus
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.203 Å
AuthorsSong, H. / Qi, J. / Shi, Y. / Gao, G.F.
CitationJournal: PLoS Pathog. / Year: 2016
Title: An Open Receptor-Binding Cavity of Hemagglutinin-Esterase-Fusion Glycoprotein from Newly-Identified Influenza D Virus: Basis for Its Broad Cell Tropism
Authors: Song, H. / Qi, J. / Khedri, Z. / Diaz, S. / Yu, H. / Chen, X. / Varki, A. / Shi, Y. / Gao, G.F.
History
DepositionOct 9, 2015Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Mar 23, 2016Provider: repository / Type: Initial release
Revision 1.1Sep 5, 2018Group: Data collection / Database references / Derived calculations
Category: citation / pdbx_struct_oper_list
Item: _citation.journal_abbrev / _citation.page_first ..._citation.journal_abbrev / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _pdbx_struct_oper_list.symmetry_operation
Revision 2.0Jul 29, 2020Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / chem_comp / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_struct_special_symmetry / pdbx_validate_close_contact / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _atom_site_anisotrop.U[1][1] / _atom_site_anisotrop.U[1][2] / _atom_site_anisotrop.U[1][3] / _atom_site_anisotrop.U[2][2] / _atom_site_anisotrop.U[2][3] / _atom_site_anisotrop.U[3][3] / _atom_site_anisotrop.pdbx_auth_asym_id / _atom_site_anisotrop.pdbx_auth_atom_id / _atom_site_anisotrop.pdbx_auth_comp_id / _atom_site_anisotrop.pdbx_auth_seq_id / _atom_site_anisotrop.pdbx_label_asym_id / _atom_site_anisotrop.pdbx_label_atom_id / _atom_site_anisotrop.pdbx_label_comp_id / _atom_site_anisotrop.type_symbol / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_struct_special_symmetry.label_asym_id / _pdbx_validate_close_contact.auth_asym_id_1 / _pdbx_validate_close_contact.auth_asym_id_2 / _pdbx_validate_close_contact.auth_seq_id_1 / _pdbx_validate_close_contact.auth_seq_id_2 / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Nov 8, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_leaving_atom_flag
Revision 2.2Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Hemagglutinin-esterase
B: Hemagglutinin-esterase
C: Hemagglutinin-esterase
D: Hemagglutinin-esterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)131,83616
Polymers124,4814
Non-polymers7,35512
Water11,331629
1
A: Hemagglutinin-esterase
B: Hemagglutinin-esterase
hetero molecules

A: Hemagglutinin-esterase
B: Hemagglutinin-esterase
hetero molecules

A: Hemagglutinin-esterase
B: Hemagglutinin-esterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)198,72724
Polymers186,7226
Non-polymers12,00518
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation7_454-z-1/2,-x,y-1/21
crystal symmetry operation10_554-y,z+1/2,-x-1/21
Buried area46650 Å2
ΔGint101 kcal/mol
Surface area68130 Å2
MethodPISA
2
C: Hemagglutinin-esterase
D: Hemagglutinin-esterase
hetero molecules

C: Hemagglutinin-esterase
D: Hemagglutinin-esterase
hetero molecules

C: Hemagglutinin-esterase
D: Hemagglutinin-esterase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)196,78124
Polymers186,7226
Non-polymers10,05918
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation5_555z,x,y1
crystal symmetry operation9_555y,z,x1
Buried area42670 Å2
ΔGint51 kcal/mol
Surface area68370 Å2
MethodPISA
Unit cell
Length a, b, c (Å)164.825, 164.825, 164.825
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number198
Space group name H-MP213
Components on special symmetry positions
IDModelComponents
11D-842-

HOH

21D-844-

HOH

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Components

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Hemagglutinin- ... , 2 types, 4 molecules ACBD

#1: Protein Hemagglutinin-esterase


Mass: 46456.988 Da / Num. of mol.: 2 / Fragment: UNP residues 19-445
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza D virus (D/swine/Oklahoma/1334/2011)
Strain: D/swine/Oklahoma/1334/2011 / Gene: HEF / Plasmid: PFASTBAC1 / Cell line (production host): HI5 / Production host: TRICHOPLUSIA NI (cabbage looper) / References: UniProt: K9LG83
#2: Protein Hemagglutinin-esterase


Mass: 15783.566 Da / Num. of mol.: 2 / Fragment: UNP residues 464-612
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Influenza D virus (D/swine/Oklahoma/1334/2011)
Strain: D/swine/Oklahoma/1334/2011 / Gene: HEF / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: K9LG83

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Sugars , 4 types, 12 molecules

#3: Polysaccharide alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 1072.964 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3[DManpa1-6]DManpa1-6DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,6,5/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3-3-3/a4-b1_b4-c1_c6-d1_d3-e1_d6-f1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(6+1)][a-D-Manp]{[(3+1)][a-D-Manp]{}[(6+1)][a-D-Manp]{}}}}}}LINUCSPDB-CARE
#4: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 5 / Source method: obtained synthetically
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#5: Polysaccharide 9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonic acid-(2-3)-beta-D- ...9-O-acetyl-5-acetamido-3,5-dideoxy-D-glycero-alpha-D-galacto-non-2-ulopyranosonic acid-(2-3)-beta-D-galactopyranose-(1-3)-2-azidoethyl 2-acetamido-2-deoxy-beta-D-glucopyranoside


Type: oligosaccharide / Mass: 785.706 Da / Num. of mol.: 2 / Source method: obtained synthetically
DescriptorTypeProgram
WURCS=2.0/3,3,2/[a2122h-1b_1-5_1*OCCN=^ZN=N_2*NCC/3=O][a2112h-1b_1-5][Aad21122h-2a_2-6_5*NCC/3=O_9*OCC/3=O]/1-2-3/a3-b1_b3-c2WURCSPDB2Glycan 1.1.0
[][<C2N3>]{[(1+1)][b-D-GlcpNAc]{[(3+1)][b-D-Galp]{[(3+2)][a-D-Neup5Ac9Ac]{}}}}LINUCSPDB-CARE
#6: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 1 types, 629 molecules

#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 629 / Source method: isolated from a natural source / Formula: H2O

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3 Å3/Da / Density % sol: 58.97 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop
Details: 0.1M PCTP (Propionic acid, Cacodylate, Bis-tris propane system) buffer pH 8.5, 22.5%(w/v) PEG1500

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 1 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Aug 2, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.2→50 Å / Num. obs: 75303 / % possible obs: 100 % / Redundancy: 9.7 % / Rmerge(I) obs: 0.093 / Rsym value: 0.093 / Net I/σ(I): 25.2
Reflection shellResolution: 2.2→2.28 Å / Redundancy: 9.7 % / Rmerge(I) obs: 0.565 / Mean I/σ(I) obs: 5 / Rsym value: 0.565 / % possible all: 100

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Processing

Software
NameVersionClassification
HKL-2000data collection
PHENIX(phenix.refine: 1.9_1692)refinement
PDB_EXTRACT3.15data extraction
PHASERphasing
HKL-2000data reduction
HKL-2000data scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1FLC

1flc


Resolution: 2.203→49.697 Å / SU ML: 0.25 / Cross valid method: NONE / σ(F): 1.34 / Phase error: 25.03 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2442 3664 4.87 %
Rwork0.2045 71639 -
obs0.2064 75303 99.96 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 203.56 Å2 / Biso mean: 48.0466 Å2 / Biso min: 22.45 Å2
Refinement stepCycle: final / Resolution: 2.203→49.697 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8700 0 492 629 9821
Biso mean--58.38 43.66 -
Num. residues----1146
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.019428
X-RAY DIFFRACTIONf_angle_d1.50112790
X-RAY DIFFRACTIONf_chiral_restr0.0641442
X-RAY DIFFRACTIONf_plane_restr0.011610
X-RAY DIFFRACTIONf_dihedral_angle_d13.6663462
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 26

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.2026-2.23150.27861480.245827112859100
2.2315-2.26210.29661320.236727182850100
2.2621-2.29440.28151370.235927812918100
2.2944-2.32870.2421200.227527392859100
2.3287-2.36510.29871670.223726902857100
2.3651-2.40380.28671380.236427452883100
2.4038-2.44530.30431240.230727412865100
2.4453-2.48980.27971250.230127512876100
2.4898-2.53760.30961470.235427482895100
2.5376-2.58940.27591530.23127072860100
2.5894-2.64570.30081600.233327072867100
2.6457-2.70730.27241210.229227772898100
2.7073-2.7750.31181550.231427552910100
2.775-2.850.25771420.223627252867100
2.85-2.93390.26641410.22427162857100
2.9339-3.02850.23711370.227427732910100
3.0285-3.13680.27421410.221427442885100
3.1368-3.26230.28061300.228627702900100
3.2623-3.41080.25891260.210727742900100
3.4108-3.59050.21871470.192527492896100
3.5905-3.81540.19781370.180927682905100
3.8154-4.10990.21871800.178227552935100
4.1099-4.52320.21221250.162427832908100
4.5232-5.17720.20461690.159127802949100
5.1772-6.52040.2161310.198528232954100
6.5204-49.70920.24261310.21372909304099
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.2698-0.0577-0.13650.34120.36461.13560.02350.0099-0.1050.0222-0.0156-0.15220.09520.1148-0.01310.3448-0.0179-0.02580.29250.0420.35525.06191.4564-57.3588
20.6976-0.02460.06891.4631-0.36630.32770.085-0.15150.13790.11470.05150.27120.0032-0.1354-0.01330.3665-0.0835-0.00950.35830.03230.3371-11.0531.4512-33.7584
30.10560.16190.09390.1990.09450.07920.0669-0.2527-0.06150.54660.51660.8868-0.3834-0.55020.02930.6770.08210.26540.65940.13290.7229-26.396144.6714-20.8567
40.3635-0.35750.40831.123-0.46320.56340.0679-0.0956-0.03730.12750.25440.7504-0.2464-0.3823-0.0370.3842-0.02970.07970.47940.13640.6754-25.259439.5251-32.9707
50.41310.3479-0.27920.3856-0.06510.93870.0005-0.3128-0.1380.23740.55641.12270.0627-1.2951-0.21750.461-0.10850.01780.79020.28720.9576-34.090836.5111-32.4231
60.2820.04490.01090.66330.1120.34410.0251-0.03270.0120.1009-0.05530.1020.02540.02520.00190.3253-0.0721-0.04890.28590.0490.2877-8.131415.7117-44.7204
71.8594-0.95570.91411.0544-0.0190.9589-0.2780.3206-0.4307-0.02280.18520.12580.67910.26730.05640.71190.1450.08730.4223-0.03340.659311.0383-26.8456-82.2367
81.22612.18892.41293.91274.38285.42080.11041.1329-1.2212-0.6452-0.08510.05411.503-0.41340.03521.38160.3322-0.04581.0453-0.40021.184217.8877-38.2225-93.3622
90.0930.7472-0.16676.9286-1.53920.33770.214-0.04680.19090.1271-0.1932-0.6327-0.15160.3511-0.01420.79410.0230.03210.7340.05771.000820.2864-28.4542-80.0737
100.7917-0.1426-0.57480.98350.58931.5878-0.04320.003-0.1736-0.01290.0349-0.20190.18860.31450.00570.37530.0141-0.01280.37120.01270.40535.878-3.682-73.781
113.81651.18671.82393.1077-0.06211.0116-0.05080.1014-0.531-0.62020.4366-0.30620.42920.4519-0.0210.7270.18460.16140.7216-0.06841.009626.089-27.312-93.325
121.2066-0.00540.20791.01660.04771.0841-0.050.25690.4902-0.19380.06790.0563-0.3806-0.13750.02720.4713-0.0587-0.01010.32760.02570.38955.012616.2016-8.1179
130.73880.21250.03481.11970.19271.0735-0.0302-0.0027-0.05620.02870.0532-0.0680.02160.03690.01860.25670.04080.01040.2680.01950.2637-2.3131-28.6771-45.5493
140.7852-0.12140.44671.0501-0.29130.9148-0.11470.0928-0.0484-0.08530.05580.2541-0.0099-0.18120.01640.30860.0236-0.01550.28890.03220.3454-18.2062-31.275-53.1654
150.98270.0540.1291.02610.03590.4952-0.04730.1987-0.2382-0.14090.10220.27210.0463-0.0498-0.00670.3382-0.0141-0.03470.3305-0.00180.44-25.6591-47.7231-55.0374
161.3576-0.0932-0.17720.90760.62740.3845-0.1430.1059-0.0126-0.0262-0.07460.2528-0.0635-0.1545-0.07610.3621-0.0413-0.02470.47310.02190.6031-36.4631-41.6807-53.4977
170.73380.04860.04091.29860.35280.7406-0.02920.0367-0.0213-0.00940.02380.295-0.0337-0.11860.01380.26150.02160.00110.28950.0240.3331-19.0211-33.493-48.4247
181.98490.30161.01541.7215-0.79561.3238-0.00770.33950.1921-0.0162-0.0242-0.1601-0.20860.0743-0.00910.3171-0.01730.01370.31690.04110.3449-1.0292-17.6092-54.7821
190.41520.20460.03941.28070.50681.1839-0.03150.0170.2232-0.0003-0.0374-0.1097-0.21440.03990.00190.36730.04310.00420.2921-0.02020.2969-4.5631-12.8267-33.2559
200.39760.74990.63020.9690.7760.7419-0.0664-0.02080.07660.0583-0.03870.1840.03750.14390.08180.45970.01470.00480.3946-0.02250.3629-4.232-0.043-19.789
211.99740.5878-1.16961.6957-0.27882.0746-0.0085-0.06910.39660.06020.0183-0.0117-0.53590.21730.00610.5662-0.07330.03850.3131-0.01430.49248.89822.7575-4.0855
223.6777-5.2787-5.18097.72697.84228.37620.0106-0.99941.09350.6223-0.0472-0.029-1.4193-0.272-0.10081.4845-0.289-0.07020.8477-0.10741.181415.585434.52936.8901
230.2114-0.92490.3034.5294-1.47240.47980.08280.30240.2119-0.0915-0.1817-0.48750.18820.3209-0.02750.84070.0093-0.00840.72430.09310.751317.846524.3825-6.2493
240.23450.90560.94081.09071.13870.68270.0828-0.08220.02140.0039-0.0502-0.03990.03060.01590.00030.47940.03390.03360.4785-0.00190.43881.842-5.144-18.6448
252.86631.22281.71741.21281.27182.03190.1114-0.0373-0.05450.1135-0.1205-0.1211-0.04210.02780.00280.3362-0.0073-0.02770.3409-0.00290.37853.70073.6423-6.7258
263.22910.1911-0.13281.4723-0.29151.382-0.2746-0.2830.060.5083-0.2113-0.5467-0.55670.40690.00280.654-0.2292-0.16660.5897-0.00860.735923.300423.25077.5667
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 3 through 71 )A0
2X-RAY DIFFRACTION2chain 'A' and (resid 72 through 170 )A0
3X-RAY DIFFRACTION3chain 'A' and (resid 171 through 201 )A0
4X-RAY DIFFRACTION4chain 'A' and (resid 202 through 243 )A0
5X-RAY DIFFRACTION5chain 'A' and (resid 244 through 273 )A0
6X-RAY DIFFRACTION6chain 'A' and (resid 274 through 427 )A0
7X-RAY DIFFRACTION7chain 'B' and (resid 9 through 28 )B0
8X-RAY DIFFRACTION8chain 'B' and (resid 29 through 38 )B0
9X-RAY DIFFRACTION9chain 'B' and (resid 39 through 46 )B0
10X-RAY DIFFRACTION10chain 'B' and (resid 47 through 131 )B0
11X-RAY DIFFRACTION11chain 'B' and (resid 132 through 157 )B0
12X-RAY DIFFRACTION12chain 'C' and (resid 3 through 30 )C0
13X-RAY DIFFRACTION13chain 'C' and (resid 31 through 111 )C0
14X-RAY DIFFRACTION14chain 'C' and (resid 112 through 146 )C0
15X-RAY DIFFRACTION15chain 'C' and (resid 147 through 243 )C0
16X-RAY DIFFRACTION16chain 'C' and (resid 244 through 273 )C0
17X-RAY DIFFRACTION17chain 'C' and (resid 274 through 343 )C0
18X-RAY DIFFRACTION18chain 'C' and (resid 344 through 366 )C0
19X-RAY DIFFRACTION19chain 'C' and (resid 367 through 397 )C0
20X-RAY DIFFRACTION20chain 'C' and (resid 398 through 427 )C0
21X-RAY DIFFRACTION21chain 'D' and (resid 9 through 28 )D0
22X-RAY DIFFRACTION22chain 'D' and (resid 29 through 38 )D0
23X-RAY DIFFRACTION23chain 'D' and (resid 39 through 46 )D0
24X-RAY DIFFRACTION24chain 'D' and (resid 47 through 82 )D0
25X-RAY DIFFRACTION25chain 'D' and (resid 83 through 131 )D0
26X-RAY DIFFRACTION26chain 'D' and (resid 132 through 157 )D0

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