#1: Journal: To Be Published Title: Crystal structures of ricin toxin's enzymatic subunit (RTA) in complex with neutralizing and non-neutralizing single-chain antibodies Authors: Rudolph, M.J. / Mantis, N.
History
Deposition
Sep 29, 2015
Deposition site: RCSB / Processing site: RCSB
Revision 1.0
Aug 3, 2016
Provider: repository / Type: Initial release
Revision 1.1
Apr 12, 2017
Group: Database references / Source and taxonomy / Structure summary
Mass: 28903.562 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Ricinus communis (castor bean) / Production host: Escherichia coli (E. coli) / References: UniProt: P02879, rRNA N-glycosylase
#2: Antibody
F8(JOB10) VHHantibody
Mass: 13653.028 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vicugna pacos (alpaca) / Production host: Escherichia coli (E. coli)
Mass: 18.015 Da / Num. of mol.: 122 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.27 Å3/Da / Density % sol: 45.79 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion / pH: 5.5 Details: 100 mM BisTris pH 5.5, 25% PEG 3000, 260 mM NaCl, and 10 mM Tris (2carboxyethyl)phosphine hydrochloride
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