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Yorodumi- PDB-5daa: E177K MUTANT OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRI... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5daa | ||||||
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Title | E177K MUTANT OF D-AMINO ACID AMINOTRANSFERASE COMPLEXED WITH PYRIDOXAMINE-5'-PHOSPHATE | ||||||
Components | D-AMINO ACID AMINOTRANSFERASE | ||||||
Keywords | TRANSFERASE / AMINOTRANSFERASE / PYRIDOXAL PHOSPHATE / TRANSAMINASE | ||||||
Function / homology | Function and homology information D-amino acid biosynthetic process / D-alanine-2-oxoglutarate aminotransferase activity / D-amino-acid transaminase / D-amino acid catabolic process / pyridoxal phosphate binding Similarity search - Function | ||||||
Biological species | Bacillus sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Peisach, D. / Ringe, D. | ||||||
Citation | Journal: Biochemistry / Year: 1999 Title: Effects of the E177K mutation in D-amino acid transaminase. Studies on an essential coenzyme anchoring group that contributes to stereochemical fidelity. Authors: van Ophem, P.W. / Peisach, D. / Erickson, S.D. / Soda, K. / Ringe, D. / Manning, J.M. #1: Journal: Biochemistry / Year: 1995 Title: Crystal Structure of a D-Amino Acid Aminotransferase: How the Protein Controls Stereoselectivity Authors: Sugio, S. / Petsko, G.A. / Manning, J.M. / Soda, K. / Ringe, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5daa.cif.gz | 110.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5daa.ent.gz | 87.1 KB | Display | PDB format |
PDBx/mmJSON format | 5daa.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5daa_validation.pdf.gz | 451 KB | Display | wwPDB validaton report |
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Full document | 5daa_full_validation.pdf.gz | 457.2 KB | Display | |
Data in XML | 5daa_validation.xml.gz | 21.3 KB | Display | |
Data in CIF | 5daa_validation.cif.gz | 28 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/da/5daa ftp://data.pdbj.org/pub/pdb/validation_reports/da/5daa | HTTPS FTP |
-Related structure data
Related structure data | 1daaS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31721.223 Da / Num. of mol.: 2 / Mutation: E177K Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus sp. (bacteria) / Strain: YM-1 / Production host: Escherichia coli (E. coli) / References: UniProt: P19938, D-amino-acid transaminase #2: Chemical | #3: Water | ChemComp-HOH / | Sequence details | THE 5 C-TERMINAL RESIDUES WERE NOT OBSERVED IN THE ELECTRON DENSITY MAPS. THEY ARE NOT INCLUDED IN ...THE 5 C-TERMINAL RESIDUES WERE NOT OBSERVED IN THE ELECTRON DENSITY MAPS. THEY ARE NOT INCLUDED IN THIS ENTRY. THE LAST FIVE RESIDUES WERE NOT SEEN IN THE DENSITY MAPS | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.3 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / pH: 8.5 Details: PDA INACTIVATED PROTEIN WAS CONCENTRATED TO 30 MG/ML IN 0.1 M POTASSIUM PHOSPHATE BUFFER PH 7.6 CONTAINING 50 UM PLP AND 0.001 BETA-MERCAPTOETHANOL. CRYSTALS WERE THEN GROWN BY THE HANGING ...Details: PDA INACTIVATED PROTEIN WAS CONCENTRATED TO 30 MG/ML IN 0.1 M POTASSIUM PHOSPHATE BUFFER PH 7.6 CONTAINING 50 UM PLP AND 0.001 BETA-MERCAPTOETHANOL. CRYSTALS WERE THEN GROWN BY THE HANGING DROP METHOD IN 27% PEG 4000, 0.4 M SODIUM ACETATE, AND 0.1 M TRIS-CHLORIDE PH 8.5., vapor diffusion - hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS pH: 7.3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 278 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Jan 12, 1998 |
Radiation | Monochromator: FLAT CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→30 Å / Num. all: 38415 / Num. obs: 38415 / % possible obs: 93.1 % / Observed criterion σ(I): 0 / Redundancy: 2.8 % / Rmerge(I) obs: 0.144 / Net I/σ(I): 9.9 |
Reflection shell | Resolution: 2.9→3.2 Å / Rmerge(I) obs: 0.337 / Mean I/σ(I) obs: 4.9 / % possible all: 94.1 |
Reflection | *PLUS Num. obs: 13649 / Num. measured all: 39415 |
Reflection shell | *PLUS % possible obs: 94.1 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1DAA Resolution: 2.9→30 Å / Rfactor Rfree error: 0.007 / Data cutoff high rms absF: 10000000 / Isotropic thermal model: GROUP / Cross valid method: THROUGHOUT / σ(F): 0 / Details: USED SOLVENT MASK DURING REFINEMENT
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 28.12 Å2 / ksol: 0.328 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.9→30 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: RESTRAINTS / Rms dev Biso : 2 Å2 / Rms dev position: 2 Å / Weight Biso : 50 / Weight position: 50 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.9→3.08 Å / Rfactor Rfree error: 0.023 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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