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- PDB-5d9q: Crystal Structure of the BG505 SOSIP gp140 HIV-1 Env trimer in Co... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5d9q | ||||||||||||||||||
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Title | Crystal Structure of the BG505 SOSIP gp140 HIV-1 Env trimer in Complex with the Broadly Neutralizing Fab PGT122 and scFv NIH45-46 | ||||||||||||||||||
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![]() | IMMUNE SYSTEM / HIV Env glycoprotein / broadly neutralizing antibodies | ||||||||||||||||||
Function / homology | ![]() opsonization / positive regulation of B cell activation / regulation of complement activation / peptide cross-linking / phagocytosis, recognition / CD22 mediated BCR regulation / complement activation / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / phagocytosis, engulfment ...opsonization / positive regulation of B cell activation / regulation of complement activation / peptide cross-linking / phagocytosis, recognition / CD22 mediated BCR regulation / complement activation / Fc epsilon receptor (FCERI) signaling / Classical antibody-mediated complement activation / phagocytosis, engulfment / Initial triggering of complement / leukocyte migration / Fc-gamma receptor signaling pathway involved in phagocytosis / Fc-epsilon receptor signaling pathway / FCGR activation / regulation of immune response / Role of phospholipids in phagocytosis / Role of LAT2/NTAL/LAB on calcium mobilization / Scavenging of heme from plasma / positive regulation of plasma membrane raft polarization / positive regulation of receptor clustering / negative regulation of inflammatory response to antigenic stimulus / immunoglobulin complex, circulating / immunoglobulin receptor binding / positive regulation of establishment of T cell polarity / FCERI mediated Ca+2 mobilization / FCGR3A-mediated IL10 synthesis / host cell endosome membrane / receptor-mediated endocytosis / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / complement activation, classical pathway / Regulation of Complement cascade / Cell surface interactions at the vascular wall / FCERI mediated MAPK activation / FCGR3A-mediated phagocytosis / antigen binding / B cell receptor signaling pathway / Regulation of actin dynamics for phagocytic cup formation / FCERI mediated NF-kB activation / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / antibacterial humoral response / clathrin-dependent endocytosis of virus by host cell / viral protein processing / blood microparticle / defense response to bacterium / fusion of virus membrane with host plasma membrane / external side of plasma membrane / innate immune response / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane / structural molecule activity / virion membrane / extracellular space / extracellular exosome / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||||||||||||||
Biological species | ![]() ![]() ![]() | ||||||||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||||||||
![]() | Julien, J.-P. / Stanfield, R.L. / Ward, A.B. / Wilson, I.A. | ||||||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Minimally Mutated HIV-1 Broadly Neutralizing Antibodies to Guide Reductionist Vaccine Design. Authors: Jardine, J.G. / Sok, D. / Julien, J.P. / Briney, B. / Sarkar, A. / Liang, C.H. / Scherer, E.A. / Henry Dunand, C.J. / Adachi, Y. / Diwanji, D. / Hsueh, J. / Jones, M. / Kalyuzhniy, O. / ...Authors: Jardine, J.G. / Sok, D. / Julien, J.P. / Briney, B. / Sarkar, A. / Liang, C.H. / Scherer, E.A. / Henry Dunand, C.J. / Adachi, Y. / Diwanji, D. / Hsueh, J. / Jones, M. / Kalyuzhniy, O. / Kubitz, M. / Spencer, S. / Pauthner, M. / Saye-Francisco, K.L. / Sesterhenn, F. / Wilson, P.C. / Galloway, D.M. / Stanfield, R.L. / Wilson, I.A. / Burton, D.R. / Schief, W.R. | ||||||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 792.3 KB | Display | ![]() |
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PDB format | ![]() | 657.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 10 MB | Display | ![]() |
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Full document | ![]() | 10 MB | Display | |
Data in XML | ![]() | 73.6 KB | Display | |
Data in CIF | ![]() | 112 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5kzcC ![]() 4ncoS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Envelope glycoprotein ... , 2 types, 6 molecules GAJBCK
#1: Protein | Mass: 52862.793 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 17031.395 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Antibody , 3 types, 9 molecules LEMHFNDIO
#3: Antibody | Mass: 22712.082 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #4: Antibody | Mass: 25448.717 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #5: Antibody | Mass: 26304.242 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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-Sugars , 7 types, 60 molecules ![](data/chem/img/NAG.gif)
#6: Polysaccharide | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #7: Polysaccharide | Source method: isolated from a genetically manipulated source #8: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #9: Polysaccharide | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D- ...alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #10: Polysaccharide | Source method: isolated from a genetically manipulated source #11: Polysaccharide | Source method: isolated from a genetically manipulated source #12: Sugar | ChemComp-NAG / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 6.28 Å3/Da / Density % sol: 80.43 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 2.4 M ammonium sulfate, 0.1 M Tris, pH 8.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 22, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03324 Å / Relative weight: 1 |
Reflection | Resolution: 4.4→40 Å / Num. obs: 67706 / % possible obs: 99.9 % / Redundancy: 20 % / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 4.4→4.5 Å / Mean I/σ(I) obs: 2 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4NCO Resolution: 4.4→39.894 Å / SU ML: 1.3 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 44.7 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.4→39.894 Å
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Refine LS restraints |
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LS refinement shell |
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