Microtubule-associatedproteins1A/1Blightchain3B / Autophagy-related protein LC3 B / Autophagy-related ubiquitin-like modifier LC3 B / MAP1 light ...Autophagy-related protein LC3 B / Autophagy-related ubiquitin-like modifier LC3 B / MAP1 light chain 3-like protein 2 / MAP1A/MAP1B light chain 3 B / MAP1A/MAP1B LC3 B / Microtubule-associated protein 1 light chain 3 beta
Mass: 15121.482 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MAP1LC3B, MAP1ALC3 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9GZQ8
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9 Å / Relative weight: 1
Reflection
Resolution: 1.53→50 Å / Num. obs: 17994 / % possible obs: 96.4 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.052 / Net I/σ(I): 49.7
Reflection shell
Resolution: 1.53→1.56 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.098 / % possible all: 97.9
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Phasing
Phasing
Method: molecular replacement
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Processing
Software
Name
Version
Classification
REFMAC
5.6.0117
refinement
HKL-2000
datacollection
HKL-2000
datascaling
MOLREP
phasing
PDB_EXTRACT
3.15
dataextraction
HKL-2000
datareduction
Coot
modelbuilding
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.53→19.58 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.919 / SU B: 1.24 / SU ML: 0.048 / Cross valid method: THROUGHOUT / ESU R: 0.091 / ESU R Free: 0.093 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.21473
898
5 %
RANDOM
Rwork
0.17473
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-
-
obs
0.17672
17088
96.16 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK